Design of Peptide Inhibitors for the Importin α/β Nuclear Import Pathway by Activity-Based Profiling

Chemistry and Biology

Volumn 15, Issue 9, 2008, Pages 940-949

  Kosugi, Shunichi ^a,b   Hasebe, Masako ^a   Entani, Tetsuyuki ^b   Takayama, Seiji ^b   Tomita, Masaru ^a   Yanagawa, Hiroshi ^a  

^a KEIO UNIVERSITY   (Japan)

^b NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

CELLBIO; CHEMBIO

Indexed keywords

KARYOPHERIN ALPHA; KARYOPHERIN BETA; PEPTIDE;

ACTIVE TRANSPORT; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL STRAIN 3T3; CHEMISTRY; DRUG ANTAGONISM; DRUG DESIGN; DRUG EFFECT; ENZYME SPECIFICITY; METABOLISM; MOLECULAR GENETICS; MOUSE; SACCHAROMYCES CEREVISIAE;

ACTIVE TRANSPORT, CELL NUCLEUS; ALPHA KARYOPHERINS; AMINO ACID SEQUENCE; ANIMALS; BETA KARYOPHERINS; DRUG DESIGN; MICE; MOLECULAR SEQUENCE DATA; NIH 3T3 CELLS; PEPTIDES; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY;

EID: 51649083754     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chembiol.2008.07.019     Document Type: Article

References (44)

1. Arnold M., Nath A., Wohlwend D., and Kehlenbach R.H. Transportin is a major nuclear import receptor for c-Fos: a novel mode of cargo interaction. J. Biol. Chem. 281 (2006) 5492-5499
2. Cansizoglu A.E., Lee B.J., Zhang Z.C., Fontoura B.M., and Chook Y.M. Structure-based design of a pathway-specific nuclear import inhibitor. Nat. Struct. Mol. Biol. 14 (2007) 452-454
3. Conti E., Uy M., Leighton L., Blobel G., and Kuriyan J. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α. Cell 94 (1998) 193-204
4. Fagerlund R., Kinnunen L., Köhler M., Julkunen I., and Melén K. NF-κB is transported into the nucleus by importin α3 and importin α4. J. Biol. Chem. 280 (2005) 15942-15951
5. Forwood J.K., Lam M.H., and Jans D.A. Nuclear import of Creb and AP-1 transcription factors requires importin-β 1 and Ran but is independent of importin-α. Biochemistry 40 (2001) 5208-5217
6. Fox R.J., Davis S.C., Mundorff E.C., Newman L.M., Gavrilovic V., Ma S.K., Chung L.M., Ching C., Tam S., Muley S., et al. Improving catalytic function by ProSAR-driven enzyme evolution. Nat. Biotechnol. 25 (2007) 338-344
7. Gilchrist D., Mykytka B., and Rexach M. Accelerating the rate of disassembly of karyopherin.cargo complexes. J. Biol. Chem. 277 (2002) 18161-18172
8. Gilchrist D., and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin α in the nucleoplasm. J. Biol. Chem. 278 (2003) 51937-51949
9. Görlich D., and Kutay U. Transport between the cell nucleus and the cytoplasm. Annu. Rev. Cell Dev. Biol. 15 (1999) 607-660
10. Görlich D., Prehn S., Laskey R.A., and Hartmann E. Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79 (1994) 767-778
11. Görlich D., Vogel F., Mills A.D., Hartmann E., and Laskey R.A. Distinct functions for the two importin subunits in nuclear protein import. Nature 377 (1995) 246-248
12. Harel A., and Forbes D.J. Importin β: conducting a much larger cellular symphony. Mol. Cell 16 (2004) 319-330
13. Harreman M.T., Hodel M.R., Fanara P., Hodel A.E., and Corbett A.H. The auto-inhibitory function of importin α is essential in vivo. J. Biol. Chem. 278 (2003) 5854-5863
14. Hodel A.E., Harreman M.T., Pulliam K.F., Harben M.E., Holmes J.S., Hodel M.R., Berland K.M., and Corbett A.H. Nuclear localization signal receptor affinity correlates with in vivo localization in Saccharomyces cerevisiae. J. Biol. Chem. 281 (2006) 23545-23556
15. Hodel M.R., Corbett A.H., and Hodel A.E. Dissection of a nuclear localization signal. J. Biol. Chem. 276 (2001) 1317-1325
16. Hood J.K., and Silver P.A. Cse1p is required for export of Srp1p/importin-α from the nucleus in Saccharomyces cerevisiae. J. Biol. Chem. 273 (1998) 35142-35146
17. Hood J.K., Casolari J.M., and Silver P.A. Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-alpha export. J. Cell Sci. 113 (2000) 1471-1480
18. Jans D.A., Xiao C.Y., and Lam M.H. Nuclear targeting signal recognition: a key control point in nuclear transport?. Bioessays 22 (2000) 532-544
19. Ladbury J.E., Lemmon M.A., Zhou M., Green J., Botfield M.C., and Schlessinger J. Measurement of the binding of tyrosyl phosphopeptides to SH2 domains: a reappraisal. Proc. Natl. Acad. Sci. USA 92 (1995) 3199-3203
20. Lange A., Mills R.E., Lange C.J., Stewart M., Devine S.E., and Corbett A.H. Classical nuclear localization signals: definition, function, and interaction with importin α. J. Biol. Chem. 282 (2007) 5101-5105
21. Lin Y.Z., Yao S.Y., Veach R.A., Torgerson T.R., and Hawiger J. Inhibition of nuclear translocation of transcription factor NF-κ B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence. J. Biol. Chem. 270 (1995) 14255-14258
22. Mildvan A.S. Inverse thinking about double mutants of enzymes. Biochemistry 43 (2004) 14517-14520
23. Miyamoto Y., Hieda M., Harreman M.T., Fukumoto M., Saiwaki T., Hodel A.E., Corbett A.H., and Yoneda Y. Importin α can migrate into the nucleus in an importin β- and Ran-independent manner. EMBO J. 21 (2002) 5833-5842
24. Nagoshi E., Imamoto N., Sato R., and Yoneda Y. Nuclear import of sterol regulatory element-binding protein-2, a basic helix-loop-helix-leucine zipper (bHLH-Zip)-containing transcription factor, occurs through the direct interaction of importin β with HLH-Zip. Mol. Biol. Cell 10 (1999) 2221-2233
25. Neduva V., and Russell R.B. Peptides mediating interaction networks: new leads at last. Curr. Opin. Biotechnol. 17 (2006) 465-471
26. Pollard V.W., Michael W.M., Nakielny S., Siomi M.C., Wang F., and Dreyfuss G. A novel receptor-mediated nuclear protein import pathway. Cell 86 (1996) 985-994
27. Privé G.G., and Melnick A. Specific peptides for the therapeutic targeting of oncogenes. Curr. Opin. Genet. Dev. 16 (2006) 71-77
28. Robbins J., Dilworth S.M., Laskey R.A., and Dingwall C. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64 (1991) 615-623
29. Saijou E., Itoh T., Kim K.W., Iemura S., Natsume T., and Miyajima A. Nucleocytoplasmic shuttling of the zinc finger protein EZI Is mediated by importin-7-dependent nuclear import and CRM1-independent export mechanisms. J. Biol. Chem. 282 (2007) 32327-32337
30. Sandberg W.S., and Terwilliger T.C. Engineering multiple properties of a protein by combinatorial mutagenesis. Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 8367-8371
31. Sato A.K., Viswanathan M., Kent R.B., and Wood C.R. Therapeutic peptides: technological advances driving peptides into development. Curr. Opin. Biotechnol. 17 (2006) 638-642
32. Schlenstedt G., Hurt E., Doye V., and Silver P.A. Reconstitution of nuclear protein transport with semi-intact yeast cells. J. Cell Biol. 123 (1993) 785-798
33. Sekimoto T., Imamoto N., Nakajima K., Hirano T., and Yoneda Y. Extracellular signal-dependent nuclear import of Stat1 is mediated by nuclear pore-targeting complex formation with NPI-1, but not Rch1. EMBO J. 16 (1997) 7067-7077
34. Siomi M.C., Fromont M., Rain J.C., Wan L., Wang F., Legrain P., and Dreyfuss G. Functional conservation of the transportin nuclear import pathway in divergent organisms. Mol. Cell. Biol. 18 (1998) 4141-4148
35. Solsbacher J., Maurer P., Vogel F., and Schlenstedt G. Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin α. Mol. Cell. Biol. 20 (2000) 8468-8479
36. Torgerson T.R., Colosia A.D., Donahue J.P., Lin Y.Z., and Hawiger J. Regulation of NF-kappa B, AP-1, NFAT, and STAT1 nuclear import in T lymphocytes by noninvasive delivery of peptide carrying the nuclear localization sequence of NF-κ B p50. J. Immunol. 161 (1998) 6084-6092
37. Truant R., Fridell R.A., Benson R.E., Bogerd H., and Cullen B.R. Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol. Cell. Biol. 18 (1998) 1449-1458
38. Vargo M.A., Nguyen L., and Colman R.F. Subunit interface residues of glutathione S-transferase A1-1 that are important in the monomer-dimer equilibrium. Biochemistry 43 (2004) 3327-3335
39. Waldmann I., Walde S., and Kehlenbach R.H. Nuclear import of c-Jun is mediated by multiple transport receptors. J. Biol. Chem. 282 (2007) 27685-27692
40. Weis K. Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle. Cell 112 (2003) 441-451
41. Wells J.A. Additivity of mutational effects in proteins. Biochemistry 29 (1990) 8509-8517
42. Wells J.A., and McClendon C.L. Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature 450 (2007) 1001-1009
43. Yamasaki H., Sekimoto T., Ohkubo T., Douchi T., Nagata Y., Ozawa M., and Yoneda Y. Zinc finger domain of Snail functions as a nuclear localization signal for importin β-mediated nuclear import pathway. Genes Cells 10 (2005) 455-464
44. Yashiroda Y., and Yoshida M. Nucleo-cytoplasmic transport of proteins as a target for therapeutic drugs. Curr. Med. Chem. 10 (2003) 741-748