Use of pyrosequencing of 16S rRNA fragments to differentiate between bacteria responsible for neonatal sepsis

Journal of Molecular Diagnostics

Volumn 7, Issue 1, 2005, Pages 105-110

  Jordan, Jeanne A ^a,b   Butchko, Allyson R ^b   Durso, Mary Beth ^b  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

AMPLICON; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD SAMPLING; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA SEQUENCE; GRAM NEGATIVE BACTERIUM; HUMAN; INTERMETHOD COMPARISON; LISTERIA; NEWBORN; NEWBORN SEPSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DATABASE; RNA SEQUENCE; SEQUENCE ANALYSIS; STAPHYLOCOCCUS; STREPTOCOCCUS;

EID: 14644422591     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-1578(10)60015-3     Document Type: Article

References (25)

1. Cerase PA: Neonatal sepsis. J Perinatal Neonatal Nursing 1989, 3:48-57
2. Gerdes JS: Clinicopathologic approach to the diagnosis of neonatal sepsis. Clin Perinatol 1991, 18:361-381
3. Klein JO, Marcy SM. Bacterial sepsis and meningitis. Infectious Diseases of the Fetus and Newborn. Edited by Remington J, Klein J. Philadelphia, W.B. Saunders, 1990, p 601
4. Witek-Janusek L, Cusack C: Neonatal sepsis: confronting the challenge. Crit Care Nurs Clin North Am 1994, 6:405-419
5. Freedman RM, Ingram DL, Gross I, Ehrenkranz RA, Warshaw JB, Baltimore RS: A half century of neonatal sepsis at Yale: 1928 to 1978. Am J Dis Child 1981, 135:140-144
6. Miller JM, O'Hara CM: Manual and automated systems for microbial identification. Manual of Clinical Microbiology, ed 7. Edited by Murray PR, Baron EJ, Pfaller MA, Pfaller M, Tenover F, Yolken R. Washington, DC, American Society for Microbiology, 1999, pp 193-201
7. Brown DR, Kutler D, Rai B, Chan T, Cohen M: Perinatal/neonatal clinical presentation: bacterial concentration and blood volume required for a positive blood culture. J Perinatol 1995, 15:157-159
8. Dietzman DE, Fischer GW, Schoenknecht FD: Neonatal Escherichia coli septicemia: bacterial counts in blood. J Pediatr 1974, 85:128-130
9. Washington II JA, Ilstrup DM: Blood cultures: issues and controversies. Rev Infect Dis 1986, 8:792-802
10. Cavaliere TA: Pharmacologic treatment of neonatal sepsis: antimicrobial agents and immunotherapy. JOGNN 1995, 24:647-658
11. Schuchat A, Whitney C, Zangwill K: Prevention of perinatal group B streptococcal disease: a public health perspective. MMWR Recomm Rep 1996, 45:1-24
12. Wegman ME: Annual summary of vital statistics: 1992. Pediatrics 1993, 92:743-754
13. Kotloff KL, Blackmon LR, Tenney JH, Rennels MB, Morris Jr JG: Nosocomial sepsis in the neonatal intensive care unit. South Med J 1989, 82:699-704
14. Ehrlich GD, Greenberg SJ: PCR-Based Diagnostics in Infectious Disease. Boston, Blackwell Scientific Publications, 1994, pp 633-664
15. Reischl U. Rapid cycle real-time PCR: methods and applications. Microbiology and Food Analysis. Edited by Rischl U, Wittwer C, Cockerill F. Berlin, Springer-Verlag 2002, pp 1-258
16. Bottger EC: Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol Lett 1989, 53:171-176
17. Jordan JA, Durso MB: Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia. J Clin Microbiol 2000, 38:2574-2578
18. Wilson KH, Blitchington RB, Greene RC: Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J Clin Microbiol 1990, 28:1942-1946
19. Grahn N, Olofsson M, Ellnebo-Svedlund K, Monstein HJ, Jonasson J: Identification of mixed bacterial DNA contamination in broad-range PCR amplification of 16S rDNA V1 and V3 variable regions by pyrosequencing of cloned amplicons. FEMS Microbiol Lett 2003, 219:87-91
20. Jonasson J, Olofsson M, Monstein HJ: Classification, identification, and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments. APMIS 2002, 110:263-272
21. Tarnberg M, Jakobsson T, Jonasson J, Forsum U: Identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions. APMIS 2002, 110:802-810
22. Greisen K, Loeffelholz M, Purohit A, Leong D: PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J Clin Microbiol 1994, 32:335-351
23. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Rapp BA, Wheeler DL: GenBank. Nucleic Acids Res 2000, 28:15-18
24. Galperin MY. The Molecular Biology Database Collection: 2004 up-date. Nucleic Acids Res 2004, 32:D3-D22
25. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 1990, 215:403-410