Functional substitution of the transient membrane-anchor domain in Escherichia coli FtsY with an N-terminal hydrophobic segment of Streptomyces lividans FtsY

FEMS Microbiology Letters

Volumn 287, Issue 1, 2008, Pages 85-90

  Maeda, Isamu ^a   Hirata, Asumi ^a   Shoji, Miki ^a   Ueda, Shunsaku ^a   Yoshida, Kazuyuki ^a  

^a UTSUNOMIYA UNIVERSITY   (Japan)

Author keywords

Escherichia coli; FtsY; Protein secretion; Signal recognition particle receptor; Streptomyces lividans

Indexed keywords

BACTERIAL PROTEIN; BETA LACTAMASE; CHIMERIC PROTEIN; FTSY PROTEIN; PEPTIDASE; SIGNAL RECOGNITION PARTICLE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CELL MEMBRANE; CONTROLLED STUDY; DELETION MUTANT; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUSION; GRAM POSITIVE BACTERIUM; HYDROPHOBICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; STREPTOMYCES LIVIDANS;

BACTERIAL PROTEINS; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; HYDROPHOBICITY; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; STREPTOMYCES LIVIDANS;

ESCHERICHIA COLI; POSIBACTERIA; STREPTOMYCES LIVIDANS;

EID: 50949109215     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2008.01297.x     Document Type: Article

References (21)

1. Angelini S, Boy D, Schiltz E Koch HG (2006) Membrane binding of the bacterial signal recognition particle receptor involves two distinct binding sites. J Cell Biol 174 : 715 724.
2. Arvidson CG, Powers T, Walter P So M (1999) Neisseria gonorrhoeae PilA is an FtsY homolog. J Bacteriol 181 : 731 739.
3. Beha D, Deitermann S, Muller M Koch HG (2003) Export of β-lactamase is independent of the signal recognition particle. J Biol Chem 278 : 22161 22167.
4. Bibi E, Herskovits AA, Bochkareva ES Zelazny A (2001) Putative integral membrane SRP receptors. Trends Biochem Sci 26 : 15 16.
5. Egea PF, Shan SO, Napetschnig J, Savage DF, Walter P Stroud RM (2004) Substrate twinning activates the signal recognition particle and its receptor. Nature 427 : 215 221.
6. Eitan A Bibi E (2004) The core Escherichia coli signal recognition particle receptor contains only the N and G domains of FtsY. J Bacteriol 186 : 2492 2494.
7. Focia PJ, Shepotinovskaya IV, Seidler JA Freymann DM (2004) Heterodimeric GTPase core of the SRP targeting complex. Science 303 : 373 377.
8. Haddad A, Rose RW Pohlschroder M (2005) The Haloferax volcanii FtsY homolog is critical for haloarchaeal growth but does not require the A domain. J Bacteriol 187 : 4015 4022.
9. Keen NT, Tamaki S, Kobayashi D Trollinger D (1988) Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70 : 191 197.
10. Kyte J Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157 : 105 132.
11. Ladefoged SA Christiansen G (1997) A GTP-binding protein of Mycoplasma hominis: a small sized homolog to the signal recognition particle receptor FtsY. Gene 201 : 37 44.
12. Luirink J, ten Hagen-Jongman CM, van der Weijden CC, Oudega B, High S, Dobberstein B Kusters R (1994) An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY. EMBO J 13 : 2289 2296.
13. Maeda I, Hirose N, Yamashiro H et al 2003) Comparative study of the N-terminal hydrophilic region in Streptomyces lividans and E. coli FtsY. Curr Microbiol 47 : 22 25.
14. Miller JD, Bernstein HD Walter P (1994) Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor. Nature 367 : 657 659.
15. Miller JD, Tajima S, Lauffer L Walter P (1995) The β subunit of the signal recognition particle receptor is a transmembrane GTPase that anchors the α subunit, a peripheral membrane GTPase, to the endoplasmic reticulum membrane. J Cell Biol 128 : 273 282.
16. Millman JS Andrews DW (1999) A site-specific, membrane-dependent cleavage event defines the membrane binding domain of FtsY. J Biol Chem 274 : 33227 33234.
17. Millman JS, Qi HY, Vulcu F, Bernstein HD Andrews DW (2001) FtsY binds to the Escherichia coli inner membrane via interactions with phosphatidylethanolamine and membrane proteins. J Biol Chem 276 : 25982 25989.
18. Palomino C Mellado RP (2005) The Streptomyces lividans cytoplasmic signal recognition particle receptor FtsY is involved in protein secretion. J Mol Microbiol Biotechnol 9 : 57 62.
19. Rubio A, Jiang X Pogliano K (2005) Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa. J Bacteriol 187 : 5000 5002.
20. Zanen G, Antelmann H, Westers H, Hecker M, van Dijl JM Quax WJ (2004) FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion. J Bacteriol 186 : 5956 5960.
21. Zelazny A, Seluanov A, Cooper A Bibi E (1997) The NG domain of the prokaryotic signal recognition particle receptor, FtsY, is fully functional when fused to an unrelated integral membrane polypeptide. Proc Natl Acad Sci USA 94 : 6025 6029.