Agarose Gel electrophoresis and fluorometric assays for the determination of DNA cross-linking induced by semustine

2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008

Volumn , Issue , 2008, Pages 322-325

  Tang, Wei ^a   Zhao, Li Jiao ^a   Zhong, Ru Gang ^a  

^a BEIJING UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Alkylating agents; DNA cross linking; Induction period; Semustine

Indexed keywords

ASSAYS; BIOCOMMUNICATIONS; BIOINFORMATICS; BIOMEDICAL ENGINEERING; COLLOIDS; CONCENTRATION (PROCESS); DNA; ELECTROCHEMISTRY; ELECTROPHORESIS; GELATION; GELS; GENES; INFORMATION SCIENCE;

ALKYLATING AGENTS; DNA CROSS-LINKING; INDUCTION PERIOD; INTERNATIONAL CONFERENCES; SEMUSTINE;

NUCLEIC ACIDS;

EID: 50949096948     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/ICBBE.2008.82     Document Type: Conference Paper

References (14)

1. S. K.Carter, F. M. Jr. Schabel, L. E Broder, T. P. Johnston, "1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and other nitrosoureas in cancer treatment," A review. Adv. Cancer Res. vol. 16, pp. 273-332, 1988.
2. A. Naghipur, M. G. Ikonomou, P. Kebarle, J. W. Lown, "Mechanism of action of (2-haloethyl)-nitrosoureas on DNA: discrimination between alternative pathways of DNA base modification by 1,3-bis-(2-fluoroethyl)-1-nitrosourea by using specific deuterium labeling and identification of products by HPLC/Tandem mass spectrometry," J. Am. Chem. Soc. vol. 112, pp. 3178-3187, 1990.
3. W. J. Bodell, "Repair of DNA alkylation products formed in 9L cell lines treated with 1-(2-chloroethyl)-1-nitrosourea," Mutation Research. vol. 522, pp. 85-92, 2003.
4. P. L. Fischhaber, A. S. Gall, J. A. Duncan, P. B. Hopkins, "Direct demonstration in synthetic oligonucleotides that N,N′-Bis(2-chloroethyl)- nitrosourea cross-Links N1 of deoxyguanosine to N3 of deoxycytidine on opposite strands of duplex DNA," Cancer Res. vol. 59, pp. 4363-4367, 1999.
5. H. Ueda-Kawamitsu, T. A. Lawson, P. R. Gwilt, "In vitro pharmacokinetics and pharmacody-mics of 1,3-bis(2-chloroethyl)-1- nitrosourea(BCNU)," Biochemical Pharmacology. vol. 63, pp. 1209-1218, 2002.
6. M. E. Dolan, A. E. Pegg, N. K. Hora, L. C. Erickson, "Effect of O6-methylguanine on DNA interstrand cross-link formation by chloroethylnitrosoureas and 2-chloroethyl(methylsulfonyl)-methanesulfonate, " Cancer Res. vol. 48, pp. 3603-3606, 1988.
7. T. R. Cech, "Alkaline gel electrophoresis of deoxyribonucleic acid photoreacted with trimethylpsoralen:rapid and sensitive detection of interstrand cross-Links," Biochemistry. vol. 20, pp. 1431-1437, 1981.
8. J. Basak, "Inter-strand cross-linking of V&Co cholerae DNA induced by furazolidone: a quantitative assay by four simple methods," Mutation Research. vol. 327, pp. 5-15, 1995.
9. M. K. Dennehy, R. N. Loeppky, "Mass spectrometric methodology for the determination of glyoxaldeoxyguanosine and O6- Hydroxyethyldeoxyguanosine DNA adducts produced by nitrosamine bident carcinogens," Chem. Res. Toxicol. vol. 18, pp. 556-565, 2005.
10. P. G. Penketh, K. Shyam, A. C. Sartorelli, "Fluorometric assay for the determination of DNA-DNA cross-links utilizing Hoechst 33258 at neutral pH values," Anal Biochem. vol. 252, pp. 210-213, 1997.
11. P. G. Penketh, K. Shyam, A. C. Sartorelli, "Comparison of DNA lesions produced by tumorinhibitory 1,2-Bis(sulfonyl)hydrazines and chloroethylnitrosoureas," Biochemical Pharmacology. vol. 59, pp. 283-291, 2000.
12. T. M. Hayes, J. Bartley, G. P. Parsons, G. K. Eaglesham, "Mechanism of action of Fotemustine, a new chloroethylnitrosourea anticancer agent: evidence for the formation of two DNA-reactive intermediates contributing to cytotoxicity," Biochemistry. vol. 36, pp. 10646-10654, 1997.
13. W. J. Bodell, K. Pongracz, "Chemical synthesis and detection of 1-[N3-2-deoxycytidyl], 2-[N1-2deoxyguanosinyl]-ethane in DNA reacted with 1-(2-chloroethyl)-1-nitrosourea," Chem. Res. Toxicol. vol. 6, pp. 434-438, 1993.
14. F. X. Chen, W. J. Bodell, G. Liang, B. Gold, "Reaction of N-(2-chloroethyl)-N-nitrosoureas with DNA: effect of buffers on DNA adduction, cross-Linking, and cytotoxicity," Chem. Res. Toxicol. vol. 9, pp. 208-214, 1996.