Suppressive effect and mechanism of saxatilin, a disintegrin from Korean snake (Gloydius saxatilis), in vascular smooth muscle cells

Toxicon

Volumn 52, Issue 3, 2008, Pages 474-480

  Sohn, Young Doug ^a   Cho, Kil Sang ^b   Sun, Sae Ah ^a   Sung, Hee Jin ^a   Kwak, Kyu Won ^b   Hong, Sung Yu ^c   Kim, Doo Sik ^d   Chung, Kwang Hoe ^b  

^a Yonsei University College of Medicine   (South Korea)

^b Department of Biochemistry   (South Korea)

^c BioBud Inc   (South Korea)

^d Yonsei University   (South Korea)

Author keywords

v 3; Cell cycle; De adhesion; Disintegrin; Focal adhesion; Integrin; Proliferation; Saxatilin; Smooth muscle cells

Indexed keywords

ACTIN; ALPHA2BBETA3 INTEGRIN; BASIC FIBROBLAST GROWTH FACTOR; CASPASE; CYCLIN D1; CYCLIN D2; CYCLIN DEPENDENT KINASE INHIBITOR 1A; CYCLIN DEPENDENT KINASE INHIBITOR 1B; CYCLIN E; DISINTEGRIN; FOCAL ADHESION KINASE; INTEGRIN; MITOGEN ACTIVATED PROTEIN KINASE; PAXILLIN; PLATELET DERIVED GROWTH FACTOR BB; SAXATILIN; UNCLASSIFIED DRUG; VERY LATE ACTIVATION ANTIGEN 5; VITRONECTIN RECEPTOR;

ARTICLE; CELL ADHESION; CELL CYCLE; CELL PROLIFERATION; CONTROLLED STUDY; CORONARY ARTERY; CYTOSKELETON; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; FOCAL ADHESION; HUMAN; HUMAN CELL; IC 50; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SNAKE; VASCULAR SMOOTH MUSCLE;

ANIMALS; CELL ADHESION; CELL CYCLE; CELL PROLIFERATION; CELLS, CULTURED; DISINTEGRINS; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FOCAL ADHESION PROTEIN-TYROSINE KINASES; HUMANS; MUSCLE, SMOOTH, VASCULAR; MYOCYTES, SMOOTH MUSCLE; SNAKE VENOMS; VIPERIDAE;

GLOYDIUS SAXATILIS;

EID: 50549086551     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2008.06.020     Document Type: Article

References (16)

1. Bishop G.G., McPherson J.A., Sanders J.M., Hesselbacher S.E., Feldman M.J., McNamara C.A., Gimple L.W., Powers E.R., Mousa S.A., and Sarembock I.J. Selective αvβ3-receptor blockade reduces macrophage infiltration and restenosis after balloon angioplasty in the atherosclerotic rabbit. Circulation 103 (2001) 1906-1911
2. Chay K.O., Park S.S., and Mushnski J.F. Linkage of caspase-mediated degradation of paxillin to apoptosis in Ba/F3 murine pro-B lymphocytes. J. Biol. Chem. 17 (2002) 14521-14529
3. Davenpeck K.L., Marcinkiewicz C., Wang D., Niculescu R., Shi Y., Martin J.L., and Zalewski A. Regional differences in integrin expression; role of α5β1 in regulating SMC function. Circ. Res. 88 (2001) 352-358
4. Giancotti F.G., and Rouslahti E. Integrin signaling. Science 285 (1999) 1028-1032
5. Hong S.Y., Koh Y.S., Chung K.H., and Kim D.S. Snake venom disintegrin, saxatilin, inhibits platelet aggregation, human umbilical vein endothelial cell proliferation, and SMC migration. Thromb. Res. 105 (2002) 79-86
6. Hong S.Y., Sohn Y.D., Chung K.H., and Kim D.S. Structural and functional significance of disulfide bonds in saxatilin, a 7.7 kDa disintegrin. Biochem. Biophys. Res. Commun. 293 (2002) 530-536
7. Hong S.Y., Lee H., You W.K., Chung K.H., Kim D.S., and Song K. The snake venom disintegrin salmosin induces apoptosis by disassembly of focal adhesion in bovine capillary endothelial cells. Biochem. Biophys. Res. Commun. 302 (2003) 502-508
8. Jean-Baptist M. Anoikis in the cardio vascular system known and unknown extracellular mediators. Arterioscler. Thromb. Vasc. Biol. 12 (2003) 2146-2154
9. Kang I.C., Lee Y.D., and Kim D.S. A novel disintegrin salmosin inhibits tumor angiogenesis. Cancer Res. 59 (1999) 3754-3760
10. Kang I.C., Kim D.S., Jang Y.S., and Chung K.H. Suppressive mechanism of salmosin, a novel disintegrin in B16 melanoma cell metastasis. Biochem. Biophys. Res. Commun. 275 (2000) 169-173
11. Kim K.S., Kim D.S., Chung K.H., and Park Y.S. Inhibition of angiogenesis and tumor progression by hydrodynamic cotransfection of angiostatin K1-3, endostatin, and saxatilin genes. Cancer Gene Ther. 13 (2006) 563-571
12. Mawatari K., Liu B., and Kent K.C. Activation of integrin receptor is required for growth factor-induced SMC dysfunction. J. Vasc. Surg. 31 (2000) 375-381
13. Ruegg C., and Mariotti A. Vascular integrins: pleiotropic adhesion and signaling molecules in vascular homeostasis and angiogenesis. Cell. Mol. Life Sci. 60 (2003) 1135-1157
14. Sohn Y.D., Hong S.Y., Cho K.S., Choi W.S., Song S.W., Bae J.S., Kim D.S., and Chung K.H. Acute and repeated dose toxicity studies of recombinant saxatilin, a disintegrin from the Korean snake (Gloydius saxatilis). Toxicon 51 (2008) 406-417
15. Wilson S.H., Ljubimov A.V., Morla A.O., Caballero S., Shaw L.C., Spoerri P.E., Tarnuzzer R.W., and Grant M.B. Fibronectin fragments promote human retinal endothelial cell adhesion and proliferation and ERK activation through alpha5 beta1 integrin and PI 3-kinase. Investig. Ophthalmol. Vis. Sci. 44 (2003) 1704-1715
16. Wu W.B., Peng H.C., and Hwang T.F. Disintegrin causes proteolysis o β-catenin and apoptosis of endothelial cells: involvement of cell-cell-ECM interactions in regulating cell viability. Exp. Cell Res. 286 (2003) 115-127