A simple analysis system for the estimation of recombination efficiency using fluorescence-activated cell sorting

Journal of Biotechnology

Volumn 127, Issue 3, 2007, Pages 373-384

  Kim, Min Soo ^a   Kim, Won Hee ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

FACS; Incompatibility; Recombinase mediated cassette exchange; Recombination efficiency; Site specific recombinase

Indexed keywords

CELLS; FLUORESCENCE; MUTAGENESIS;

RECOMBINASE-MEDIATED CASSETTE EXCHANGE; RECOMBINATION EFFICIENCY; SITE-SPECIFIC RECOMBINASE;

BIOTECHNOLOGY;

CRE RECOMBINASE; SPACER DNA;

ACCURACY; ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; EMBRYO; EXPERIMENTATION; FLUORESCENCE ACTIVATED CELL SORTING; GENE CASSETTE; GENE SEQUENCE; GENETIC INCOMPATIBILITY; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; IN VIVO STUDY; MUTANT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WILD TYPE;

CELL LINE; FLOW CYTOMETRY; GENETIC VECTORS; HUMANS; INTEGRASES; MUTATION; RECOMBINATION, GENETIC;

EID: 37849188673     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.07.027     Document Type: Article

References (18)

1. Albert H., Dale E.C., Lee E., and Ow D.W. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J. 7 (1995) 649-659
2. Arad U. Modified Hirt procedure for rapid purification of extrachromosomal DNA from mammalian cells. BioTechniques 24 (1998) 760-762
3. Baer A., and Bode J. Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes. Curr. Opin. Biotechnol. 12 (2001) 473-480
4. Bell A.C., and Felsenfeld G. Stopped at the border: boundaries and insulators. Curr. Opin. Genet. Dev. 9 (1999) 191-198
5. Branda C.S., and Dymecki S.M. Talking about a revolution: the impact of site-specific recombinases on genetic analyses in mice. Dev. Cell. 6 (2004) 7-28
6. Buchholz F., Angrand P.O., and Stewart A.F. Improved properties of FLP recombinase evolved by cycling mutagenesis. Nat. Biotechnol. 16 (1998) 657-662
7. Festenstein R., Tolaini M., Corbella P., Mamalaki C., Parrington J., Fox M., Miliou A., Jones M., and Kioussis D. Locus control region function and heterochromatin-induced position effect variegation. Science 271 (1996) 1123-1125
8. Hoess R.H., Ziese M., and Sternberg N. P1 site-specific recombination: nucleotide sequence of the recombining sites. Proc. Natl. Acad. Sci. 79 (1982) 3398-3402
9. Kolot M., Meroz A., and Yagil E. Site-specific recombination in human cells catalyzed by the wild-type integrase protein of coliphage HK022. Biotechnol. Bioeng. 84 (2003) 56-60
10. Langer S.J., Ghafoori A.P., Byrd M., and Leinwand L. A genetic screen identifies novel non-compatible loxP sites. Nucleic Acids Res. 30 (2002) 3067-3077
11. Lee G., and Saito I. Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination. Gene 216 (1998) 55-65
12. Lee J., and Jayaram M. Role of partner homology in DNA recombination. J. Biol. Chem. 270 (1995) 4042-4052
13. McLeod M., Craft S., and Broach J.R. Identification of the crossover site during FLP-mediated recombination in the Saccharomyces cerevisiae plasmid 2 microns circle. Mol. Cell. Biol. 6 (1986) 3357-3367
14. Park M.T., and Lee G.M. Rapid titer assay of adenovirus containing green fluorescent protein gene using flow cytometric analysis. Bioprocess Eng. 22 (2000) 403-406
15. Ringrose L., Lounnas V., Ehrlich L., Buchholz F., Wade R., and Stewart A.F. Comparative kinetic analysis of FLP and Cre recombinases: mathematical models for DNA binding and recombination. J. Mol. Biol. 284 (1998) 363-384
16. Schlake T., and Bode J. Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 33 (1994) 12746-12751
17. Umlauf S.W., and Cox M.M. The functional significance of DNA sequence structure in a site-specific genetic recombination reaction. EMBO J. 7 (1988) 1845-1852
18. Yoon Y.G., Posfai G., Szybalski W., and Kim S.C. Cre/loxP-mediated in vivo excision of large segments from yeast genome and their amplification based on the 2 micron plasmid-derived system. Gene 223 (1998) 67-76