Purification and characterization of extracellular cholesterol oxidase from Enterobacter sp.

World Journal of Microbiology and Biotechnology

Volumn 24, Issue 10, 2008, Pages 2227-2233

  Ye, Deping ^a   Lei, Jiahong ^a   Li, Wei ^a   Ge, Fanglan ^a   Wu, Ke ^a   Xu, Wenliu ^a   Yong, Bin ^a  

^a SICHUAN NORMAL UNIVERSITY   (China)

Author keywords

Carnivore feces; Enterobacter; Extracellular cholesterol oxidase; Stability

Indexed keywords

ABSORPTION; CHOLESTEROL; CHROMATOGRAPHIC ANALYSIS; COLLOIDS; FILTRATION; FOOD ADDITIVES; GEL PERMEATION CHROMATOGRAPHY; GELATION; MONOMERS; POROUS MATERIALS; PURIFICATION; STRAIN; ULTRAFILTRATION;

BACTERIAL STRAINS; CARNIVORE FECES; CHOLESTEROL OXIDASE; CHOLESTEROL OXIDASES; CULTURE SUPERNATANT; ENTEROBACTER; EXTRACELLULAR; EXTRACELLULAR CHOLESTEROL OXIDASE; GEL FILTRATION; P H VALUES; PURIFIED ENZYMES; Q-SEPHAROSE; STABILITY;

ENZYME IMMOBILIZATION;

BACTERIA (MICROORGANISMS); ENTEROBACTER; ENTEROBACTER SP.;

EID: 50249128126     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-008-9734-2     Document Type: Article

References (19)

1. H Aihara K Watanabe R Nakamura 1988 Degradation of cholesterol in egg yolk by Rhodococcus equi no.23 J Food Sci 53 659 660
2. CC Allain LS Poon CSG Chan W Richmond PC Fu 1974 Enzymatic determination of total serum cholesterol Clin Chem 20 470 475
3. R Aono N Doukyu H Kobayashi H Nakajima K Horikoshi 1994 Oxidative bioconversion of cholesterol by Pseudomonas sp. strain ST-200 in a water-organic solvent two-phase system Appl Environ 60 2518 2523
4. Buchanan RE, Gibbons NE (1974) Bergey's manual of determinative bacteriology, Eighth edn. Williams and Wilkins Co., Baltimore, pp 290-340
5. R Bru A Sanchez-Ferrer F Garcia-Carmona 1989 Characterization of cholesterol oxidase activity in AOT-isooctane reverse micelles and its dependence on micelle size Biotechnol Lett 11 237 242
6. R Deeg J Ziegenhorn 1983 Kinetic enzymic method for automated determination of total cholesterol in serum Clin Chem 29 1798 1802
7. N Doukyu R Aono 1998 Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. strain ST-200 Appl Environ Microbiol 64 1929 1932
8. G Gadda G Wels L Pollegioni S Zucchelli D Ambrosius MS Pilone S Ghisla 1997 Characterization of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum Eur J Biochem 250 369 376
9. K Isobe N Mori N Wakao 2003 The second cholesterol oxidase produced by gamma-proteobacterium Y-134 J Biosci Bioeng 96 257 261
10. KP Kim CH Rhee HD Park 2002 Degradation of cholesterol by Bacillus subtilis SFF34 isolated from Korean traditional fermented flatfish Lett Appl Microbiol 35 468 472
11. PH Lolekha Y Jantaveesirirat 1992 Streptomyces, a superior source for cholesterol oxidase used in serum cholesterol assay J Clin Lab Anal 6 405 409
12. PH Lolekha P Srisawasdi P Jearanaikoon N Wetprasit B Sriwanthana MH Kroll 2004 Performance of four sources of cholesterol oxidase for serum cholesterol determination by the enzymatic endpoint method Clin Chim Acta 339 135 145
13. J MacLachlan AT Wotherspoon RO Ansell CJ Brooks 2000 Cholesterol oxidase: sources, physical properties and analytical applications J Steroid. Biochem Mol Biol 72 169 195
14. L Motteran MS Pilone G Molla S Ghisla LC Pollegioni 2001 Holesterol oxidase from Brevibacterium sterolicum: the relationship between covalent flavinylation and redox properties J Biol Chem 276 18024 18030
15. W Richmond 1973 Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum Clin Chem 19 1350 1356
16. NS Sampson A Vrielink 2003 Cholesterol oxidases: a study of nature's approach to protein design Acc Chem Res 36 713 722
17. PK Smith R Krohn GT Hermanson 1985 Measurement of protein using bicinchoninic acid Anal Biochem 150 76 85
18. MM Sojo RR Bru FF Garcia-Carmona 2002 Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration BMC Biotechnol 2 3
19. P Srisawasdi P Jearanaikoon MH Kroll PH Lolekha 2005 Performance characteristics of cholesterol oxidase for kinetic determination of total cholesterol J Clin Lab Anal 19 247 252