Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: Cell-linked and extracellular enzyme synthesis, purification and concentration

BMC Biotechnology

Volumn 2, Issue , 2002, Pages

  Sojo, Mar M ^a   Bru, Roque R ^b   García Carmona, Francisco F ^a  

^a UNIVERSITY OF MURCIA   (Spain)

^b UNIVERSITY OF ALICANTE   (Spain)

Author keywords

Cholesterol oxidase; Enzyme purification; Phase separation; Rhodococcus erythropolis ATCC 25544; Triton X 114

Indexed keywords

CHOLESTEROL; CHOLESTEROL OXIDASE; DETERGENT; ENZYME INDUCING AGENT; POLYSORBATE 20; TRITON X 114;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BIOMASS PRODUCTION; BIOREACTOR; CONCENTRATION RESPONSE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ENZYME ACTIVATION; ENZYME INDUCTION; ENZYME PURIFICATION; ENZYME SYNTHESIS; EXTRACTION; FERMENTATION TECHNIQUE; HEATING; INDUSTRIAL PRODUCTION; INTERMETHOD COMPARISON; NONHUMAN; PHASE SEPARATION; RHODOCOCCUS ERYTHROPOLIS; SPRAY DRYING; TEMPERATURE;

BACTERIA (MICROORGANISMS); RHODOCOCCUS; RHODOCOCCUS ERYTHROPOLIS;

EID: 3042611514     PISSN: 14726750     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6750-2-3     Document Type: Article

References (33)

1. Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC: Enzymatic determination of total serum cholesterol. Clin Chem 1974, 20:470-475
2. Kazandjian RZ, Dordich JS, Kilbanov AM: Enzymatic analysis in organic solvents. Biotechnol Bioeng 1986, 28:417-421
3. Bru R, Sanchez-Ferrer A, Garcia-Carmona F: Characterization of cholesterol oxidase activity in AOT-isooctane reverse micelles and its dependence on micelle size. Biotechnol Lett 1989, 11:237-242
4. Stadtman TC, Cherkes A, Anfinsen CB: Studies on the microbiological degradation of cholesterol. J Biol Chem 1954, 206:511-523
5. MacLachlan J, Wotherspoon ATL, Ansell RO, Brooks CJW: Cholesterol oxidase: sources, physical properties and analytical applications. J Steroid Biochem Mol Biol 2000, 72:169-195
6. Richmond W: Preparation and properties of a cholesterol oxidase from Nocardia sp and its application to the enzymatic assay of total cholesterol in serum. Clin Chem 1973, 19:1350-1356
7. Cheetham PS, Dunnill P, Lilly MD: The characterization and interconversion of three forms of cholesterol oxidase extracted from Nocardia rhodochrous. Biochem J 1982, 201:512-521
8. Kreit J, Germain P, Lefebvre G: Extracellular cholesterol oxidase from Rhodococcus sp cells. J Biotechnol 1992, 24:177-188
9. Sojo M, Bru R, López-Molina D, García-Carmona F, Argüelles JC: Cell-linked and extracellular cholesterol oxidase activities from Rhodococcus erythropolis. Isolation and physiological characterization. Appl Microbiol Biotechnol 1997, 47:583-589
10. Johnson TL, Somkuti GA: Isolation of cholesterol oxidases from Rhodococcus equi ATCC 33706. Biotechnol Appl Biochem 1991, 13:196-204
11. Aihara H, Watanabe K, Nakamura R: Characterization of production of cholesterol oxidases in three Rhodococcus strains. J Appl Bacteriol 1986, 61:269-274
12. Buckland BC, Lilly MD, Dunnil P: The kinetics of cholesterol oxidase synthesis by Nocardia rhodochrous. Biotechnol Bioeng 1976, 18:601-621
13. Sánchez-Ferrer A, Bru R, García-Carmona F: Phase separation of biomolecules in polyoxyethylene glycol nonionic detergents. Crit Rev Biochem Mol Biol 1994, 29:275-313
14. Sánchez-Ferrer A, Pérez-Gilabert M, Núñez E, Bru R, García-Carmona F: Triton X-114 phase partitioning in plant protein purification. J Chomatogr 1994, 668:75-83
15. Bru R, Sánchez-Ferrer A, Pérez-Gilabert M, López-Nicolas JM, García-Carmona F: Plant protein purification using cloud point extraction (CPE). In: Surfactants in Solution New York, Marcel Dekker Inc., 1995, 367-377
16. Ramelmeier RA, Terstappen G, Kula MR: The partitioning of cholesterol oxidase in Triton X-114 based in aqueous two-phases systems. Bioseparation 1991, 2:315-324
17. Minuth T, Thommas J, Kula MR: Extraction of cholesterol oxidase from Nocardia rhodochrous using a nonionic surfactantbased aqueous two phase systems. J Biotechnol 1995, 38:151-164
18. Minuth T, Thommas J, Kula MR: A closed concept for purification of the membrane bound cholesterol oxidase from Nocardia rhodochrous by surfactant-based cloud-point extraction organic-solvent extraction and anion-exchange chromatography. Biotechnol Appl Biochem 1996, 23:107-116
19. Minuth T, Gieren H, Pape U, Raths HC, Thommas J, Kula MR: Pilot scale processing of detergent-based aqueous two-phase systems. Biotechnol Bioeng 1997, 55:339-347
20. Murooka Y, Ishizaki T, Nimi O, Maekawa N: Cloning and expression of a Streptomyces cholesterol oxidase gene in Streptomyces lividans with plasmid pIJ 702. Appl Environ Microbiol 1986, 52:1382-1385
21. Atrat PG, Wagner B, Wagner M, Schumann G: Localization of the cholesterol oxidase in Rhodococcus erythropolis IMET 7185 studied by inmunoelectron microscopy. J Steroid Biochem Mol Biol 1992, 42:193-200
22. Cheetham PSJ, Dunnill P, Lilly MD: Extraction of cholesterol oxidase from Nocardia rhodochrous. Enzyme Microb Technol 1980, 2:201-205
23. Wilmanska D, Dziadek J, Sajduda A, Milczarek K, Jaworski A, Murooka Y: Identification of cholesterol oxidase from fastgrowing Mycobacterial strains and Rhodococcus sp. J Ferm Bioeng 1995, 79:119-124
24. Watanabe K, Shimizu H, Aihara H, Nakamura R, Suzuki K, Komagata K: Isolation and identification of cholesterol-degrading Rhodococcus strains from food of animal origin and their cholesterol oxidase activities. J Gen Appl Microbiol 1986, 32:137-147
25. Watanabe K, Aihara H, Nakagawa Y, Nakamura R, Sasaki T: Properties of the purified extracellular cholesterol oxidase from Rhodococcus equi No 23. J Agr Food Chem 1989, 37:1178-1182
26. Buckland BC, Richmond W, Dunnill P, Lilly MD: The large scale isolation of intracellular microbial enzymes: cholesterol oxidase from Nocardia. In: Industrial Aspects of Biochememistry 1974, 65-79
27. Kreit J, Lefebvre G, Germain P: Membrane-bound cholesterol oxidase from Rhodococcus sp cells. Production and extraction. J Biotechnol 1994, 33:271-282
28. Ganong BR, Delmore JP: Phase separation temperatures of mixtures of Triton X-114 and Triton X-45: application to protein separation. Anal Biochem 1991, 193:35-37
29. 5. Anal Biochem 1991, 197:125-131
30. Bordier C: Phase separation of integral membrane proteins in Triton X-114 solution. J Biol Chem 1981, 256:1604-1607
31. Sánchez-Ferrer A, Bru R, García-Carmona F: Novel procedure for extraction of a latent grape polyphenol oxidase using temperature-induced phase separation in Triton X-114. Plant Physiol 1989, 91:1481-1489
32. Bradford M: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254
33. Laemmli UK: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970, 227:680-685