Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers

Genetics and Molecular Research

Volumn 7, Issue 3, 2008, Pages 706-717

  Batista, P P ^a   Santos, J F ^a   Oliveira, N T ^a   Pires, A P D ^a   Motta, C M S ^a   Luna Alves Lima, E A ^a  

^a FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

Author keywords

Aspergillus flavus; Genetic diversity; Inter simple sequence repeats; Internal transcribed spacer; Molecular markers; Random amplified polymorphic DNA

Indexed keywords

INTERNAL TRANSCRIBED SPACER;

ARTICLE; ASPERGILLUS FLAVUS; ASPERGILLUS ORYZAE; ASPERGILLUS PARASITICUS; CONTROLLED STUDY; GENETIC VARIABILITY; INTER SIMPLE SEQUENCE REPEAT; NONHUMAN; PHYLOGENETIC TREE; RANDOM AMPLIFIED POLYMORPHIC DNA;

ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS ORYZAE; ASPERGILLUS PARASITICUS; ASPERGILLUS TAMARII;

EID: 49749146741     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/vol7-3gmr422     Document Type: Article

References (31)

1. Baleiras Couto MM, Hartog BJ, Huis In't Veld JHJ, Hofstra H, et al. (1996). Identification of spoilage yeasts in a food production chain by microsatellite polymerase chain reaction fingerprinting. Food Microbiol. 13: 59-67.
2. Bart-Delabesse E, Sarfati J, Debeaupuis JP, van Leeuwen W, et al. (2001). Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates. J. Clin. Microbiol. 39: 2683-2686.
3. Bertout S, Renaud F, Barton R, Symoens F, et al. (2001). Genetic polymorphism of Aspergillus fumigatus in clinical samples from patients with invasive aspergillosis: investigation using multiple typing methods. J. Clin. Microbiol. 39: 1731-1737.
4. Brasileiro BTRV, Coimbra MRM, Morais-Junior MA and Oliveira NT (2004). Genetic variability within Fusarium solani species as revealed by PCR-fingerprinting based on PCR markers. Braz. J. Microbiol. 35: 205-210.
5. Chen YC, Eisner JD, Kattar MM, Rassoulian-Barrett SL, et al. (2000). Identification of medically important yeasts using PCR-based detection of DNA sequence polymorphisms in the internal transcribed spacer 2 region of the rRNA genes. J. Clin. Microbiol. 38: 2302-2310.
6. Dendis M, Horvath R, Michalek J, Ruzicka F, et al. (2003). PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia. Clin. Microbiol. Infect. 9: 1191-1202.
7. Diaz-Guerra TM, Mellado E, Cuenca-Estrella M, Gaztelurrutia L, et al. (2000). Genetic similarity among one Aspergillus flavus strain isolated from a patient who underwent heart surgery and two environmental strains obtained from the operating room. J. Clin. Microbiol. 38: 2419-2422.
8. Driver F, Milner RJ and Trueman JWH (2000). A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data. Mycol. Res. 104: 134-150.
9. Geiser DM, Pitt JI and Taylor JW (1998). Cryptic speciation and recombination in the aflatoxin-producing fungus Aspergillus flavus. Proc. Natl. Acad. Sci. U. S. A. 95: 388-393.
10. Henry T, Iwen PC and Hinrichs SH (2000). Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J. Clin. Microbiol. 38: 1510-1515.
11. Hinrikson HP, Hurst SF, Lott TJ, Warnock DW, et al. (2005). Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species. J. Clin. Microbiol. 43: 2092-2103.
12. Inglis PW and Tigano MS (2006). Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS sequences. Genet. Mol. Biol. 23: 132-136.
13. Jimenez M, Rodriguez S, Mateo JJ, Gil JV, et al. (2000). Characterization of Gibberella fujikuroi complex isolates by fumonisin B1 and B2 analysis and by RAPD and restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA. Syst. Appl. Microbiol. 23: 546-555.
14. Klick MA (2002). Identification of Common Aspergillus Species. Centraalbureau voor Schimmelcultures, Utrecht.
15. Kumeda Y and Asao T (1996). Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section Flavi. Appl. Environ. Microbiol. 62: 2947-2952.
16. Kumeda Y and Asao T (2001). Heteroduplex panel analysis, a novel method for genetic identification of Aspergillus section Flavi strains. Appl. Environ. Microbiol. 67: 4084-4090.
17. Kurtzman CP, Smiley MJ, Robnett CJ and Wicklow DT (1986). DNA relatedness among wild and domesticated species in the Aspergillus flavus group. Mycologia 78: 955-959.
18. Lasker BA (2002). Evaluation of performance of four genotypic methods for studying the genetic epidemiology of Aspergillus fumigatus isolates. J. Clin. Microbiol. 40: 2886-2892.
19. Megnegneau B, Debets F and Hoekstra RF (1993). Genetic variability and relatedness in the complex group of black Aspergilli based on random amplification of polymorphic DNA. Curr. Genet. 23: 323-329.
20. Meyer W, Latouche GN, Daniel HM, Thanos M, et al. (1997). Identification of pathogenic yeasts of the imperfect genus Candida by polymerase chain reaction fingerprinting. Electrophoresis 18: 1548-1559.
21. Radford SA, Johnson EM, Leeming JP, Millar MR, et al. (1998). Molecular epidemiological study of Aspergillus fumigatus in a bone marrow transplantation unit by PCR amplification of ribosomal intergenic spacer sequences. J. Clin. Microbiol. 36: 1294-1299.
22. Raeder U and Broda P (1985). Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1: 17-20.
23. Raper KB and Fennell DI (1977). The genus Aspergillus. Williams and Wilkins Company, Baltimore, Florida.
24. Rath PM (2001). Phenotypic and genotypic characterization of reference strains of the genus Aspergillus. Mycoses 44: 65-72.
25. Rath PM, Kamphoff S and Ansorg R (1999). Value of different methods for the characterisation of Aspergillus terreus strains. J. Med. Microbiol. 48: 161-166.
26. Rath PM, Petermeier K, Verweij PE and Ansorg R (2002). Differentiation of Aspergillus ustus strains by random amplification of polymorphic DNA. J. Clin. Microbiol. 40: 2231-2233.
27. Turenne CY, Sanche SE, Hoban DJ, Karlowsky JA, et al. (1999). Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system. J. Clin. Microbiol. 37: 1846-1851.
28. Verweij PE, Meis JF, Sarfati J, Hoogkamp-Korstanje JA, et al. (1996). Genotypic characterization of sequential Aspergillus fumigatus isolates from patients with cystic fibrosis. J. Clin. Microbiol. 34: 2595-2597.
29. White TJ, Bruns T, Lee S and Taylor JW (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications (Innis MA, Gelfand DH, Sninsky JJ and White TJ, eds.). Academic Press Inc., New York, 315-322.
30. Williams JG, Kubelik AR, Livak KJ, Rafalski JA, et al. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.
31. Yuan GF, Liu CS and Chen CC (1995). Differentiation of Aspergillus parasiticus from Aspergillus sojae by random amplification of polymorphic DNA. Appl. Environ. Microbiol. 61: 2384-2387.