A pyrazine-based fluorescence-enhancing ligand with a high selectivity for thymine in AP site-containing DNA duplexes

Analytical Sciences

Volumn 24, Issue 6, 2008, Pages 693-695

  Zhao, Chunxia ^a,b   Rajendran, Arivazhagan ^a   Dai, Qing ^a   Nishizawa, Seiichi ^a   Teramae, Norio ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC COMPOUNDS; DISSOCIATION; FLUORESCENCE; LIGANDS; NUCLEOTIDES;

AMPLIFICATION PRODUCTS; DISSOCIATION CONSTANT; DNA DUPLEXES; HIGH SELECTIVITY; PYRAZINE DERIVATIVE; SENSING PROPERTY; SINGLE NUCLEOTIDE POLYMORPHISMS; THYMINE BASE;

POLYMERASE CHAIN REACTION;

EID: 49749085185     PISSN: 09106340     EISSN: 13482246     Source Type: Journal    
DOI: 10.2116/analsci.24.693     Document Type: Article

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22. 20 was done with a 24-mer forward primer (5′-GTAGTGGTAATCTACTGGGACGGA-3′) and a 23-mer reverse primer (5′-CTCGCTTAGTGCTCCCTGGGGGC-3′). The reaction solution (100 μl) contained dNTPs (2.5 mM each, 8 μl), 10 x PCR buffer (10 μl; TaKaRa), forward primer (300 pmol), reverse primer (20 pmol), template (0.2 pmol), and Ex-Taq (2.5 U; TaKaRa). PCR conditions: 94°C for 5 min, followed by 50 cycles of 96°C for 30 s, 57°C for 30 s, and 72°C for 30 s, and then 72°C for 5 min and kept at 4°C. 141-mer PCR product: 5′-GT AGT GGT AAT CTA CTG GGA CGG AAC AGC TTT GAG GTG CGT GTT TGT GCC TGT CCT GGG AYA GAC CGG CGC ACA GAG GAA GAG AAT CTC CGC AAG AAA GGG GAG CCT CAC CAC GAG CTG CCC CCA GGG AGC ACT AAG CGA G-3′ (wild type, Y = G; mutant type, Y = T); 21-mer probe DNA: 5′-GCG CCG GTC TXT CCC AGG ACA-3′ (X = AP site; Spacer-C3).