NcGRA2 as a molecular target to assess the parasiticidal activity of toltrazuril against Neospora caninum

Parasitology

Volumn 135, Issue 9, 2008, Pages 1065-1073

  Strohbusch, M ^a   Muller N ^a   Hemphill, A ^a   Greif, G ^b   Gottstein, B ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b BAYER PHARMA AG   (Germany)

Author keywords

Cell culture assay; Neospora caninum; RT PCR; Toltrazuril

Indexed keywords

GENE PRODUCT; MESSENGER RNA; PROTEIN GRA2; TOLTRAZURIL;

ANIMAL CELL; ANTIMICROBIAL ACTIVITY; ARTICLE; BIOLOGICAL MONITORING; BRADYZOITE; CELL VIABILITY; DRUG EFFECT; DRUG EFFICACY; DRUG MECHANISM; DRUG TARGETING; GENE; GENE EXPRESSION; GENETIC TRANSCRIPTION; GRA2 GENE; HOST CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; NEOSPORA CANINUM; NONHUMAN; PARASITE IDENTIFICATION; PRIORITY JOURNAL; PROTEIN TARGETING; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TACHYZOITE; VALIDATION PROCESS;

ANIMALS; CATTLE; CATTLE DISEASES; COCCIDIOSIS; COCCIDIOSTATS; NEOSPORA; POLYMERASE CHAIN REACTION; PROTOZOAN PROTEINS; TRIAZINES;

BOVINAE; NEOSPORA; NEOSPORA CANINUM;

EID: 49549119809     PISSN: 00311820     EISSN: 14698161     Source Type: Journal    
DOI: 10.1017/S0031182008004599     Document Type: Article

References (27)

1. Ammann, P., Waldvogel, A., Breyer, I., Esposito, M., Müller, N. and Gottstein, B. (2004). The role of B- and T-cell immunity in toltrazuril-treated C57BL/6 WT, microMT and nude mice experimentally infected with Neospora caninum. Parasitology Research 93, 178-187.
2. Andrianarivo, A. G., Rowe, J. D., Barr, B. C., Anderson, M. L., Packham, A. E., Sverlow, K. W., Choromanski, L., Loui, C., Grace, A. and Conrad, P. A. (2000). A POLYGEN-adjuvanted killed Neospora caninum tachyzoite preparation failed to prevent foetal infection in pregnant cattle following i.v./i.m. experimental tachyzoite challenge. International Journal for Parasitology 30, 985-990.
3. Darius, A. K., Mehlhorn, H. and Heydorn, A. O. (2004a). Effects of toltrazuril and ponazuril on Hammondia heydorni (syn. Neospora caninum) infections in mice. Parasitology Research 92, 520-522.
4. Darius, A. K., Mehlhorn, H. and Heydorn, A. O. (2004b). Effects of toltrazuril and ponazuril on the fine structure and multiplication of tachyzoites of the NC-1 strain of Neospora caninum (a synonym of Hammondia heydorni) in cell cultures. Parasitology Research 92, 453-458.
5. Dubey, J. P. (2003). Review of Neospora caninum and neosporosis in animals. Korean Journal for Parasitology 41, 1-16.
6. Dubey, J. P., Schares, G. and Ortega-Mora, L. M. (2007). Epidemiology and control of neosporosis and Neospora caninum. Clinical Microbiology Reviews 20, 323-367.
7. Ellis, J. T., Ryce, C., Atkinson, R., Balu, S., Jones, P. and Harper, P. A. (2000). Isolation, characterization and expression of a GRA2 homologue from Neospora caninum. Parasitology 120, 383-390.
8. Esposito, M., Stettler, R., Moores, S. L., Pidathala, C., Müller, N., Stachulski, A., Berry, N. G., Rossignol, J. F. and Hemphill, A. (2005). In vitro efficacies of nitazoxanide and other thiazolides against Neospora caninum tachyzoites reveal antiparasitic activity independent of the nitro group. Antimicrobial Agents and Chemotherapy 49, 3715-3723.
9. Gottstein, B., Eperon, S., Dai, W. J., Cannas, A., Hemphill, A. and Greif, G. (2001). Efficacy of toltrazuril and ponazuril against experimental Neospora caninum infection in mice. Parasitology Research 87, 43-48.
10. Gottstein, B., Razmi, G. R., Ammann, P., Sager, H. and Müller, N. (2005). Toltrazuril treatment to control diaplacental Neospora caninum transmission in experimentally infected pregnant mice. Parasitology 130, 41-48.
11. Haberkorn, A. (1996). Chemotherapy of human and animal coccidioses: state and perspectives. Parasitology Research 82, 193-199.
12. Haerdi, C., Haessig, M., Sager, H., Greif, G., Staubli, D. and Gottstein, B. (2006). Humoral immune reaction of newborn calves congenitally infected with Neospora caninum and experimentally treated with toltrazuril. Parasitology Research 99, 534-540.
13. Häsler, B., Regula, G., Stark, K. D., Sager, H., Gottstein, B. and Reist, M. (2006a). Financial analysis of various strategies for the control of Neospora caninum in dairy cattle in Switzerland. Preventive Veterinary Medicine 77, 230-253.
14. Häsler, B., Stark, K. D., Sager, H., Gottstein, B. and Reist, M. (2006b). Simulating the impact of four control strategies on the population dynamics of Neospora caninum infection in Swiss dairy cattle. Preventive Veterinary Medicine 77, 254-283.
15. Hemphill, A. and Gottstein, B. (2000). A European perspective on Neospora caninum. International Journal for Parasitology 30, 877-924.
16. Hemphill, A., Gottstein, B. and Kaufmann, H. (1996). Adhesion and invasion of bovine endothelial cells by Neospora caninum. Parasitology 112, 183-197.
17. Innes, E. A., Andrianarivo, A. G., Bjorkman, C., Williams, D. J. and Conrad, P. A. (2002). Immune responses to Neospora caninum and prospects for vaccination. Trends in Parasitology 18, 497-504.
18. Innes, E. A. and Vermeulen, A. N. (2006). Vaccination as a control strategy against the coccidial parasites Eimeria, Toxoplasma and Neospora. Parasitology 133 (Suppl.), S145-S168.
19. Kritzner, S., Sager, H., Blum, J., Krebber, R., Greif, G. and Gottstein, B. (2002). An explorative study to assess the efficacy of toltrazuril-sulfone (ponazuril) in calves experimentally infected with Neospora caninum. Annals of Clinical Microbiology and Antimicrobobials 1, 4.
20. Lindsay, D. S. and Dubey, J. P. (1989). Evaluation of anti-coccidial drugs' inhibition of Neospora caninum development in cell cultures. Journal for Parasitology 75, 990-992.
21. Lindsay, D. S., Rippey, N. S., Cole, R. A., Parsons, L. C., Dubey, J. P., Tidwell, R. R. and Blagburn, B. L. (1994). Examination of the activities of 43 chemotherapeutic agents against Neospora caninum tachyzoites in cultured cells. American Journal of Veterinary Research 55, 976-981.
22. Manger, I. D., Hehl, A., Parmley, S., Sibley, L. D., Marra, M., Hillier, L., Waterston, R. and Boothroyd, J. C. (1998). Expressed sequence tag analysis of the bradyzoite stage of Toxoplasma gondii: identification of developmentally regulated genes. Infection and Immunity 66, 1632-1637.
23. Mercier, C., Lecordier, L., Darcy, F., Deslee, D., Murray, A., Tourvieille, B., Maes, P., Capron, A. and Cesbron-Delauw, M. F. (1993). Molecular characterization of a dense granule antigen (Gra 2) associated with the network of the parasitophorous vacuole in Toxoplasma gondii. Molecular and Biochemical Parasitology 58, 71-82.
24. Müller, N., Vonlaufen, N., Gianinazzi, C., Leib, S. L. and Hemphill, A. (2002). Application of real-time fluorescent PCR for quantitative assessment of Neospora caninum infections in organotypic slice cultures of rat central nervous system tissue. Journal of Clinical Microbiology 40, 252-255.
25. Müller, N., Zimmermann, V., Forster, U., Bienz, M., Gottstein, B. and Welle, M. (2003). PCR-based detection of canine Leishmania infections in formalin-fixed and paraffin-embedded skin biopsies: elaboration of a protocol for quality assessment of the diagnostic amplification reaction. Veterinary Parasitology 114, 223-229.
26. Müller, N., Zimmermann, V., Hentrich, B. and Gottstein, B. (1996). Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. Journal of Clinical Microbiology 34, 2850-2852.
27. Vonlaufen, N., Guetg, N., Naguleswaran, A., Müller, N., Bjorkman, C., Schares, G., Von Blumroeder, D., Ellis, J. and Hemphill, A. (2004). In vitro induction of Neospora caninum bradyzoites in vero cells reveals differential antigen expression, localization, and host-cell recognition of tachyzoites and bradyzoites. Infection and Immunity 72, 576-583.