Optimized detection of respiratory viruses in nasopharyngeal secretions

New Microbiologica

Volumn 26, Issue 2, 2003, Pages 133-140

  Zavattoni, M ^a   Percivalle, E ^a   Cattaneo, E ^a   Revello, M G ^a   Torsellini, M ^a   Gerna, G ^a  

^a FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

Author keywords

Cell line mixture; immunofluorescence; Monoclonal antibody pool; Nasopharyngeal secretions; Rapid diagnosis; Respiratory viruses

Indexed keywords

MONOCLONAL ANTIBODY; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; HUMAN CELL; IMMUNOASSAY; INFLUENZA VIRUS A; INFLUENZA VIRUS B; NONHUMAN; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 2; PARAINFLUENZA VIRUS 3; PREDICTION; RESPIRATORY TRACT INFECTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS INFECTION; VIRUS ISOLATION; COMPARATIVE STUDY; CYTOPATHOGENIC EFFECT; EVALUATION; ISOLATION AND PURIFICATION; METHODOLOGY; NASOPHARYNX; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; SECRETION; SPECIES DIFFERENCE; VIROLOGY; VIRUS;

ANIMALIA; HUMAN PARAINFLUENZA VIRUS 1; HUMAN PARAINFLUENZA VIRUS 2; HUMAN PARAINFLUENZA VIRUS 3; INFLUENZA VIRUS; INFLUENZAVIRUS A; INFLUENZAVIRUS B; MUSTELA VISON; RESPIRATORY SYNCYTIAL VIRUS; SPRING VIREMIA OF CARP VIRUS;

ANTIBODIES, MONOCLONAL; CELLS, CULTURED; CYTOPATHOGENIC EFFECT, VIRAL; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; HUMANS; INFLUENZA A VIRUS; INFLUENZA B VIRUS; NASOPHARYNX; PARAINFLUENZA VIRUS 1, HUMAN; PARAINFLUENZA VIRUS 2, HUMAN; PARAINFLUENZA VIRUS 3, HUMAN; RESPIRATORY SYNCYTIAL VIRUSES; RESPIRATORY TRACT INFECTIONS; SPECIES SPECIFICITY; VIRAL PROTEINS; VIROLOGY; VIRUS DISEASES; VIRUSES;

EID: 0038481505     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. BEEKMANN, S.E., ENGLER, H.D., COLLINS, A.S., CANOSA, J., HENDERSON, D.K., and FREIFELD, A. (1996). Rapid identification of respiratory viruses: impact on isolation practices and transmission among immunocompromised pediatric patients. Infection Control and Hospital Epidemiology, 17, 581-586.
2. BRUMBACK, B.G., and WADE, C.D. (1996). Simultaneous rapid culture for four respiratory viruses in the same cell monolayer using a differential multicolored fluorescent confirmatory stain. Journal of Clinical Microbiology, 34, 798-801.
3. DOING, K.M., JERKOFSKY, M.A., Dow, E.G., and JELLISON, J.A. (1998). Use of fluorescent-antibody staining of cytocentrifuge-prepared smears in combination with cell culture for direct detection of respiratory viruses. Journal of Clinical Microbiology, 36, 2112-2114.
4. ENGLER, H.D., and PREUSS, J. (1997). Laboratory diagnosis of respiratory virus infections in 24 hours by utilizing shell vial culture. Journal of Clinical Microbiology, 35, 2165-2167.
5. FONG, C.K.Y., LEE, M.K., and GRIFFITH, B.P. (2000). Evaluation of R-Mix fresh cells in shell vials for detection of respiratory viruses. Journal of Clinical Microbiology, 38, 4660-4662.
6. FRANK, A.L., COUCH, R.B., GRIFFIS C.A., and BAXTER, B.D. (1979). Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses. Journal of Clinical Microbiology, 10, 32-36.
7. HUANG, Y.T., and TURCHEK, B.M. (2000). Mink lung cells and mixed mink lung and A549 cells for rapid detection of influenza virus and other respiratory viruses. Journal of Clinical Microbiology, 38, 422-423.
8. JOHNSTON, S.L.G., and SIEGEL, C.S. (1999). Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture and conventional culture for the detection of respiratory syncytial virus. Journal of Clinical Microbiology, 28, 2394-2397.
9. LEONARDI, G.P., LEIB, H., BIRKHEAD, G.S., SMITH, C., COSTELLO, P., and CONRON, W. (1994). Comparison of rapid detection methods for influenza A virus and their value in health-care management of institutionalized geriatric patients. Journal of Clinical Microbiology, 32, 70-74.
10. MATTHEY, S., NICHOLSON, D., RUHS, S., ALDEN, B., KNOCK, M., SCHULTZ, K., and SCHUMUECKER, A. (1992). Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies. Journal of Clinical Microbiology, 30, 540-544.
11. MENDOZA, J., NAVARRO, J.M., ROJAS, A., and DE LA ROSA, M. (1991). Evaluation of immunofluorescence, two enzyme immunoassays and the shell vial assay for detection of respiratory syncytial virus. European Journal of Clinical Microbiology and Infectious Diseases, 10, 40-42.
12. MURPHY, P., ROBERTS, Z.M., and WANER, J.L. (1996). Differential diagnosis of influenza A virus, influenza B virus, and respiratory syncytial virus infections by direct immunofluorescence using mixtures of monoclonal antibodies of different isotypes. Journal of Clinical Microbiology, 34, 1798-1800.
13. NAVARRO-MARI, J.M., SANBONMATSU-GAMEZ, S., PEREZ-RUIZ, M., and DE LA ROSA-FRAILE, M. (1999). Rapid detection of respiratory viruses by shell vial assay using simultaneous culture of HEp-2, LLC-MK2, and MDCK cells in a single vial. Journal of Clinical Microbiology, 37, 2346-2347.
14. PERCIVALLE, E., ZAVATTONI, M., REVELLO, M.G., MARIANI, G., SCARAVELLI, C., and GERNA, G. (1989). Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by immunofluorescence and ELISA does not justify discontinuation of virus isolation. Microbiologica, 12, 203-213.
15. RABALAIS, G.P., STOUT, G.G., LADD, K.L., and COST, K.M. (1992). Rapid diagnosis of respiratory virus infections by using a shell vial assay and monoclonal antibody pool. Journal of Clinical Microbiology, 30, 1505-1508.
16. REINA, J., FERNANDEZ-BACA, V., BIANCO, I., and MUNAR, M. (1997). Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (VERO) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture. Journal of Clinical Microbiology, 35, 1900-1901.
17. SCHIRM, J., LUIJT, D.S., PASTOOR, G.W., MANDEMA, J.M., and SCHRÖDER, F.P. (1992). Rapid detection of respiratory viruses using mixtures of monoclonal antibodies on shell vial cultures. Journal of Medical Virology, 38, 147-151.
18. SHIH, S.-R., TSAO, K.-C., NING, H.-C., HUANG, Y.-C., and LIN, T.-Y. (1999). Diagnosis of respiratory tract viruses in 24 h by immunofluorescent staining of shell vial cultures containing Madin-Darby canine kidney (MDCK) cells. Journal of Virological Methods, 81, 77-81.
19. SCHULTZ-CHERRY, S., DYBDAHL-SISSOKO, N., MCGREGOR, M., and HINSHAW, V.S. (1998). Mink lung epithelial cells: unique cell line that supports influenza A and B virus replication. Journal of Clinical Microbiology, 36, 3718-3720.
20. SMITH, M.C., CREUTZ, C., and HUANG, Y.T. (1991). Detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. Journal of Clinical Microbiology, 29, 463-465.
21. SMITH, C.D., CRAFT, D.W., SHIROMOTO, R.S., and YAN, P.O. (1986). Alternative cell line for virus isolation. Journal of Clinical Microbiology, 24, 265-268.
22. STOUT, S., MURPHY, M.D., LAWRENCE, S., and JULIAN, S. (1989). Evaluation of a monoclonal antibody pool for rapid diagnosis of respiratory viral infections. Journal of Clinical Microbiology, 27, 448-452.
23. WOODS, G.L., and YOUNG, A. (1988). Use of A549 cells in a clinical virology laboratory. Journal of Clinical Microbiology, 26, 1026-1028.