Influenza virus types and subtypes detection by single step single tube multiplex reverse transcription-polymerase chain reaction (RT-PCR) and agarose gel electrophoresis

Journal of Virological Methods

Volumn 99, Issue 1-2, 2002, Pages 63-70

  Poddar, Saibal K ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

Influenza virus; Multiplex PCR; Subtyping; Typing

Indexed keywords

AGAROSE; DNA FRAGMENT; PRIMER RNA; VIRUS RNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; CHROMOSOME BANDING PATTERN; CONTROLLED STUDY; DNA SEQUENCE; INFLUENZA VIRUS A; INFLUENZA VIRUS B; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; TECHNIQUE; VIRUS DETECTION; VIRUS STRAIN; VIRUS TYPING;

ELECTROPHORESIS, AGAR GEL; HUMANS; INFLUENZA A VIRUS; INFLUENZA B VIRUS; INFLUENZA, HUMAN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL;

EID: 0036027698     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(01)00380-9     Document Type: Article

References (21)

1. Comparison of reverse transcription-PCR with tissue culture and other rapid diagnostic assays for detection of type A influenza virus
2. Heterogeneity of influenza B viruses
3. Influenza surveillance-United States, 1992-93 and 1993-94
4. Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus
5. Human influenza A H5N1 virus related to a highly pathogenic avian virus
6. General concepts for PCR primer design
7. Multiplex reverse transcription-PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
8. Influenza viruses
9. Update on emerging infections from the Center for Disease Control and Prevention. Influenza: ED Considerations for the 1997-98 season
10. Detection of adenovirus using PCR and molecular beacon
11. Symmetric vs. asymmetric PCR and molecular beacon probe in the detection of a target gene of adenovirus
12. Effect of inhibitors in clinical specimens on Taq and Tth DNA polymerase-based PCR amplification of influenza A virus
13. Evaluation of PCR assays in presence of antibody to thermostable DNA polymerases for detection of microbial agents: Avoiding false negative results for specimen containing low-titer agent
14. Evaluation of a direct immunofluorescence assay, dot-blot enzyme immunoassay, and shell vial culture in the diagnosis of lower respiratory tract infections caused by influenza A virus
15. Monoclonal antibodies for rapid strain specific identification of influenza virus isolates
16. H1 and H3 specific monoclonal antibody for subtyping influenza virus
17. Application of a fluorogenic PCR assay for typing and subtyping of influenza viruses in respiratory samples
18. Rapid detection of influenza A (AH1, AH3) and B by nested-polymerase chain reaction
19. Multiplex PCR for typing and subtyping influenza and respiratory syncytial viruses
20. Typing and subtyping of influenza viruses in clinical samples by PCR
21. Clinical features and rapid viral diagnosis of human disease associated with avian A H5N1 virus