Identification of extracellular enzyme producing alkalophilic bacilli from Izmir province by 16S-ITS rDNA RFLP

Journal of Applied Microbiology

Volumn 97, Issue 4, 2004, Pages 766-773

  Akbalik, G ^a   Gunes, H ^a   Yavuz, E ^a   Yasa, I ^b   Harsa, S ^a   Elmaci, Z S ^a   Yenidunya, A Fazil ^a  

^a IZMIR INSTITUTE OF TECHNOLOGY   (Turkey)

^b EGE UNIVERSITY   (Turkey)

Author keywords

16S ITS rDNA RFLP; Alkalophilic Bacillus; Extracellular enzyme screening; Similarity analysis

Indexed keywords

BACILLI; BACTERIOLOGY; ENZYME ACTIVITY; STARCH;

16S-INTERNAL TRANSCRIBED SPACER RDNA-RESTRICTION FRAGMENT LENGTH POLYMORPHISM; ALKALOPHILIC; ALKALOPHILIC BACILLUS; ENZYME SCREENINGS; EXTRACELLULAR ENZYME SCREENING; EXTRACELLULAR ENZYMES; INTERNAL TRANSCRIBED SPACERS; POLYGALACTURONIC ACID; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; SIMILARITY ANALYSIS;

CASEIN;

AMYLASE; BACTERIAL ENZYME; CARBOXYMETHYLCELLULOSE; CASEIN; DNA 16S; GENOMIC DNA; INTERNAL TRANSCRIBED SPACER; PECTIN; POLYGALACTURONASE; POLYGALACTURONIC ACID; POLYSORBATE 20; POLYSORBATE 80; RIBOSOME DNA; STARCH; XYLAN;

MICROBIOLOGY;

ALKALOPHILIC BACILLUS; ARTICLE; BACILLUS; BACTERIUM CULTURE; BACTERIUM ISOLATION; ENZYME ACTIVITY; FECES ANALYSIS; LEATHER INDUSTRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SOIL; STRAIN DIFFERENCE;

BACILLI; BACTERIA (MICROORGANISMS); CITRUS; EQUUS CABALLUS; POSIBACTERIA;

EID: 4944247722     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2004.02357.x     Document Type: Article

References (37)

1. Anwar, A. and Saleemuddin, M. (1998) Alkaline proteases: a review. Bioresource Technology 64, 175-183.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1994) Current Protocols in Molecular Biology 3, chapter 2. NewYork: John Wiley & Sons Inc.
3. Beg, Q.K., Kapoor, M., Mahajan, L. and Hoondal, G.S. (2001) Microbial xylanases and their industrial applications: a review. Applied Microbiology and Biotechnology 56, 326-338.
4. Dalev, P.G. (1994) Utilization of waste feathers from poultry slaughter for production of a protein concentrate. Bioresource Technology 48, 265-267.
5. Fritze, D., Flossdorf, J. and Claus, D. (1990) Taxonomy of alkaliphilic Bacillus strains. International Journal of Systematic Bacteriology 40, 92-97.
6. Gessesse, A. and Gashe, B.A. (1997) Production of alkaline xylanase by an alkaliphilic Bacillus sp. isolated from an alkaline soda lake. Journal of Applied Microbiology 83, 402-406.
7. Ghanem, E.H., Al-Sayed, H.A. and Saleh, K.M. (2000) An alkalophilic thermostable lipase produced by a new isolate of Bacillus alcalophilus. World Journal of Microbiology & Biotechnology 16, 459-464.
8. Haba, E., Bresco, O., Ferrer, C., Marques, A., Basquets, M. and Manresa, A. (2000) Isolation of lipase secreting bacteria by deploying used frying oil as selective substrate. Enzyme and Microbial Technology 26, 40-44.
9. Hameed, A., Natt, M.A. and Evans, C.S. (1996) Production of alkaline protease by a Bacillus subtilis isolate for use as bating enzyme in leather treatment. World Journal of Microbiology and Biotechnology 12, 289-291.
10. Horikoshi, K. (1991) Microorganisms in Alkaline Environments. Tokyo: Kodansha.
11. Horikoshi, K. (1999a) Alkaliphiles: some applications of their products for biotechnology. Microbiology and Molecular Biology Reviews 63(No. 4), 735-750.
12. Horikoshi, K. (1999b) Extracellular enzymes: isolation procedures for alkaline enzymes. In Alkaliphiles ed. Horikhosi, K. pp. 147. Tokyo: Kodansha Ltd.
13. Hoshino, E. and Ito, S. (1997) Application of alkaline cellulases that contribute to soil removal in detergents. In Enzymes in Detergency ed. Van Ee, J.H., Misset, O. and Baas, E.J. pp. 149-174. New York: Marcel Dekker, Inc.
14. Ito, S., Kobayashi, T., Ara, K., Ozaki, K., Kawai, S. and Hatada, Y. (1998) Alkaline detergent enzymes from alkaliphiles: enzymatic properties, genetics and structures. Extremophiles 2, 185-190.
15. Jensen, M.A., Webster, J.A. and Strauss, N. (1993) Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Applied and Environmental Microbiology 59, 943-952.
16. Kashayp, D.R., Vohra, P.K., Soni, S.K. and Tewari, R. (2001) Degumming of buel (Grewia optiva) bast fibers by pectinolytic enzyme from Bacillus sp. DT7. Biotechnology Letters 23, 1297-1301.
17. Kim, T.U., Gu, B.G., Jeong, J.Y., Byun, S.M. and Shin, Y.C. (1995) Purification and characterization of a maltotetraose-forming alkaline α-amvlase from an alkalophilic Bacillus strain, GM8901. Applied and Environmental Microbiology 61(No. 8), 3105-3112.
18. Kobayashi, T., Hatada, Y., Higaki, N., Lusterio, D.D., Ozawa, T., Koike, K., Kawai, S. and Ito, S. (1999) Enzymatic properties and deduced amino acid sequence of a high-alkaline pectate lyase from an alkaliphilic Bacillus isolate. Biochimica et Biophysica Acta 1427, 145-154.
19. Kroll, R.G. (1990) Alkalophiles., In Microbiology of Extreme Environments ed. Edwards, C. pp. 55-92. New York: McGraw-Hill Publishing Co.
20. Krulwich, T.A. and Guffanti, A.A. (1989) Alkalophilic bacteria. Annual Review of Microbiology 43, 435-463.
21. Kumar, C.G. and Takagi, H. (1999) Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnology Advances 17, 561-594.
22. Loperena, L., Ferrari, M.D., Belobrajdic, L., Weyrauch, R. and Varela, H. (1994) Study of Bacillus sp. culture conditions to promote production of unhairing proteases. Revista Argentina de Microbiologia 26, 105-115.
23. Martins, R.F., Davids, W., Al-Soud, W.A., Levander, F., Radström, P. and Hatti-Kaul, R. (2001) Starch-hydrolyzing bacteria from Ethiopian soda lakes. Extremophiles 5, 135-144.
24. Mora, B., Fortina, M.G., Nicastro, G., Parini, C. and Manachini, P.L. (1998) Genotypic characterization of thermophilic bacilli: a study on new soil isolates and several reference strains. Research in Microbiology 149, 711-722.
25. Murray, R.G.E., Doetsch, R.N. and Robinow, C.F. (1994) Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology ed. Gerhardt, P., Murray, R.G.E., Wood, W.A. and Krieg, N.R. pp. 32. Washington: American Society for Microbiology.
26. Nielsen, P., Fritze, D. and Priest, F.G. (1995) Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745-1761.
27. Ntougias, S. and Russell, N.J. (2000) Bacillus sp. WW3-SN6, a novel facultatively alkaliphilic bacterium isolated from the washwaters of edible olives. Extremophiles 4, 201-208.
28. Pedersen, N.R., Wimmer, R., Matthiesen, R., Pedersen, L.H. and Gessesse, A. (2003) Synthesis of sucrose laurate using a new alkaline protease. Tetrahedron: Asymmetry 14, 667-673.
29. Reid, I. and Ricard, M. (2000) Pectinase in papermaking: solving retention problems in mechanical pulps bleached with hydrogen peroxide. Enzyme and Microbial Technology 26, 115-123.
30. Sharma, R., Chisti, Y. and Banerjee, U.C. (2001) Production, purification, characterization and applications of lipases. Biotechnology Advances 19, 627-662.
31. Singh, J., Vohra, R.M. and Sahoo, D.K. (1999) Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus. Biotechnology Letters 21, 921-924.
32. Smibert, R.M. and Krieg, N.R. (1994) Phenotypic characterization. In Methods for General and Molecular Bacteriology ed. Gerhardt, P., Murray, R.G.E., Wood, W.A. and Krieg, N.R. pp. 614. Washington: American Society for Microbiology.
33. Takami, H. and Horikoshi, K. (2000) Analysis of an alkalophilic Bacillus strain from an industrial point of view. Extremophiles 4, 99-108.
34. Takami, H., Nogi, Y. and Horikoshi, K. (1999) Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans. Extremophiles 3, 293-296.
35. Tarrand, J.J. and Gröschel, D.H.M. (1982) Rapid, modified oxidase test for oxidase variable bacterial isolates. Journal of Clinical Microbiology 16, 772-774.
36. Vedder, A. (1934) Bacillus alcalophilus n. sp.; benevens enkele ervaringen met sterk alcalische voedingsbodems. Antonie Leeuwenhoek 1, 143-147.
37. Yavuz, E., Gunes, H., Bulut, C., Harsa, S. and Yenidunya, A.F. (2004) RFLP of 16S rDNA-ITS region to differentiate lactobacilli at species level. World Journal of Microbiology and Biotechnology (in press).