Cloning and characterization of a NAD+-dependent glycerol-3-phosphate dehydrogenase gene from Candida glycerinogenes, an industrial glycerol producer

FEMS Yeast Research

Volumn 8, Issue 5, 2008, Pages 725-734

  Chen, Xianzhong ^a   Fang, Huiying ^a   Rao, Zhiming ^a   Shen, Wei ^a   Zhuge, Bin ^a   Wang, Zhengxiang ^a   Zhuge, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Candida glycerinogenes; Glycerol production; Glycerol 3 phosphate dehydrogenase; Osmoregulation; Salt stress

Indexed keywords

GLYCEROL; GLYCEROL 3 PHOSPHATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; SODIUM CHLORIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; CANDIDA; CANDIDA GLYCERINOGENES; ENZYME ACTIVITY; FUNGAL GENE; FUNGUS CULTURE; GENE SEQUENCE; GENETIC TRAIT; GENOMIC FRAGMENT; GLYCEROL 3 PHOSPHATE DEHYDROGENASE GENE; INVERSE POLYMERASE CHAIN REACTION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHENOTYPE; PROTEIN DOMAIN; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; WILD TYPE;

AMINO ACID SEQUENCE; BINDING SITES; CANDIDA; CATALYTIC DOMAIN; CLONING, MOLECULAR; DNA PRIMERS; DNA, FUNGAL; GENE DELETION; GENETIC COMPLEMENTATION TEST; GLYCEROL; GLYCEROL-3-PHOSPHATE DEHYDROGENASE (NAD+); MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OPEN READING FRAMES; PHYLOGENY; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

CANDIDA GLYCERINOGENES; SACCHAROMYCES CEREVISIAE;

EID: 49349116808     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2008.00382.x     Document Type: Article

References (29)

1. Adams A, Gottschling DE, Kaiser CA Stearns T (1997) Methods in Yeast Genetics. A Cold Spring Harbor Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Albertyn J, Hohmann S, Thevelein JM Prior BA (1994) GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Mol Cell Biol 14 : 4135 4144.
3. Ansell R, Granath K, Hohmann S, Thevelein JM Adler L (1997) The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J 16 : 2179 2187.
4. Bewley CG, Cook JL, Kusakabe S, Mukai T, Rigby DL Chambers GK (1989) Sequence structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster. Nucleic Acids Res 17 : 8553 8567.
5. Blomberg A (2000) Metabolic surprises in Saccharomyces cerevisiae during adaptation to saline conditions: questions, some answers and a model. FEMS Microbiol Lett 182 : 1 8.
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of dye binding. Anal Biochem 72 : 248 254.
7. Brewster JL, de Valoir T, Dwyer ND, Winter E Gustin MC (1993) An osmosensing signal transduction pathway in yeast. Science 259 : 1760 1763.
8. Cronwright G, Rohwer J Prior BA (2002) Metabolic control analysis of glycerol synthesis in Saccharomyces cerevisiae. Appl Environ Microbiol 68 : 4448 4456.
9. Eriksson P, Andre L, Ansell R, Blomberg A Adler L (1995) Cloning and characterization of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae, and its comparison with GPD1. Mol Microbiol 17 : 95 107.
10. Hohmann S (2002) Osmotic stress signaling and osmoadaptation in yeasts. Microbiol Mol Biol Rev 66 : 300 372.
11. Iwaki T, Kurono S, Yokose Y, Kubota K, Tamai Y Watanabe Y (2001) Cloning of glycerol-3-phosphate dehydrogenase genes (ZrGPD1 and ZrGPD2) and glycerol dehydrogenase genes (ZrGCY1 and ZrGCY2) from the salt-tolerant yeast Zygosaccharomyces rouxii. Yeast 18 : 737 744.
12. 2 supplies. Biotechnol Lett 25 : 311 314.
13. Kozak M (1991) An analysis of vertebrate mRNA sequences: intimations of translational control. J Cell Biol 115 : 887 903.
14. Marchler G, Schuller C, Adam G Ruis H (1993) A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J 12 : 1997 2003.
15. Nevoigt E Stahl U (1997) Osmoregulation and glycerol metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 21 : 231 241.
16. Ochman H, Gerber AS Hartl DL (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120 : 621 623.
17. Ohmiya R, Yamada H, Nakashima K, Aiba H Mizuno T (1995) Osmoregualtion of fission yeast: cloning of two distinct genes encoding glycerol-3-phosphate dehydrogenase, one of which is responsible for osmotolerance for growth. Mol Microbiol 18 : 963 973.
18. Otto J, Argos P Rossmann MG (1980) Prediction of secondary structural elements in glycerol-3-phosphate dehydrogenase by comparison with other dehydrogenases. Eur J Biochem 109 : 325 330.
19. Panadero J, Pallotti C, Rodriguez-Vargas S, Randez-Gil F Prieto JA (2006) A downshift in temperature activates the high osmolarity glycerol (HOG) pathway, which determines freeze tolerance in Saccharomyces cerevisiae. J Biol Chem 281 : 4638 4645.
20. Remize F, Barnavon L Dequin S (2001) Glycerol export and glycerol-3-phosphate dehydrogenase, but not glycerol phosphatase, are rate limiting for glycerol production in Saccharomyces cerevisiae. Metab Eng 3 : 301 312.
21. Sambrook J Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Sanger F, Nicklen S Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74 : 5463 5467.
23. Schmitt AP McEntee K (1996) Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 93 : 5777 5782.
24. Schuller C, Brewster JL, Alexander MR, Gustin MC Ruis H (1994) The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J 13 : 4382 4389.
25. Thomas BJ Rothstein RJ (1989) Elevated recombination rates in transcriptionally active DNA. Cell 56 : 619 630.
26. +). Yeast 21 : 119 126.
27. Wang ZX, Zhuge J, Fang H Prior BA (2001) Glycerol production by microbial fermentation: a review. Biotechnol Adv 19 : 201 223.
28. Wojda I, Alonso-Monge R, Bebelman JP, Mager WH Siderius M (2003) Response to high osmotic conditions and elevated temperature in Saccharomyces cerevisiae is controlled by intracellular glycerol and involves coordinate activity of MAP kinase pathways. Microbiology 149 : 1193 1204.
29. Zhuge J, Fang HY, Wang ZX, Chen DZ, Jin HR Gu HL (2001) Glycerol production by a novel osmotolerant yeast Candida glycerinogenes. Appl Microbiol Biotechnol 55 : 686 692.