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In previous work by Duhamel et al, 24 the Py-PGA was purified using dialysis against aqueous solution. The aqueous solution was removed by rotary evaporation, and the polymer film was further dried under vacuum at 40 °C prior to pyrene content determination. In the current work, a Labconco freeze-drying system was used to recover the purified Py-PGA from aqueous solution. This difference has proven essential for accurate pyrene content determination, as it was determined that simple vacuum drying did not remove all of the water from the polymer sample. Pyrene contents determined for the previous wet polymer samples were incorrect in some cases given that the mass of the polymer was actually lower than the amount weighed. This resulted in under-estimated pyrene content for some Py-PGAs. The pyrene content has the largest effect on the Nblob parameters reported in ref 24 as calculated by eq 6. The Nblobo value for Py-PGA reported in ref 2
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24 is similar to that of 24 ± 1 obtained in the current work by analyzing the fluorescence decays acquired with our recently purchased IBH time-resolved fluorometer.
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84906375117
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were calculated using Quantitative Structure Activity Relationship (QSAR) Properties Software v7.0, Hypercube Inc., as part of the Hyperchem 7.0 package. For the calculation, the pyrene was bound by its Van der Waals surface.
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Volumes were calculated using Quantitative Structure Activity Relationship (QSAR) Properties Software v7.0, Hypercube Inc., as part of the Hyperchem 7.0 package. For the calculation, the pyrene was bound by its Van der Waals surface.
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48
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84906375114
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o(PGA-PMA) .
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o(PGA-PMA) .
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