PI-PfuI and PI-PfuII, intein-coded homing endonucleases from Pyrococcus furiosus. II. Characterization of the binding and cleavage abilities by site-directed mutagenesis

Nucleic Acids Research

Volumn 27, Issue 21, 1999, Pages 4175-4182

  Komori, Kayoko ^a   Ichiyanagi, Kenji ^a   Morikawa, Kosuke ^a   Ishino, Yoshizumi ^a  

^a BIOMOL ENG RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; ENDONUCLEASE; MUTANT PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CATALYSIS; DNA CLEAVAGE; DNA STRAND; ENZYME ACTIVITY; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN PROCESSING; PYROCOCCUS FURIOSUS; SITE DIRECTED MUTAGENESIS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; DNA; DNA-BINDING PROTEINS; ENDODEOXYRIBONUCLEASES; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTATION; NUCLEIC ACID CONFORMATION; PROTEIN SPLICING; PYROCOCCUS FURIOSUS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

PYROCOCCUS FURIOSUS;

EID: 0033230117     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/27.21.4175     Document Type: Article

References (31)

1. Mueller, J.E., Bryk, M., Loizos, N. and Belfort, M. (1993) In Linn, S.M., Lloyd, R.S. and Roberts, R.J. (eds), Nucleases. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 111-143.
2. Belfort, M. and Roberts, R.J. (1997) Nucleic Acids Res., 25, 3379-3388.
3. Belfort, M. and Perlman, P.S. (1995) J. Biol. Chem., 270, 30237-30240.
4. Duan, X., Gimble, F.S. and Quiocho, F.A. (1997) Cell, 89, 555-564.
5. Heath, P.J., Stephens, K.M., Monnat, R.J.,Jr and Stoddard, B.L. (1997) Nature Struct. Biol., 4, 468-476.
6. Silva, G.H., Dalgaard, J.Z., Belfort, M. and Roey, P.V. (1999) J. Mol. Biol., 286, 1123-1136.
7. Jurica, M.S., Monnat, R.T., Jr and Stoddard, B.L. (1998) Mol. Cell, 2, 469-476.
8. Flick, K.E., Jurica, M.S., Monnat, R.T.,Jr and Stoddard, B.L. (1998) Nature, 394, 96-101.
9. Riera, J., Robb, F.T., Weiss, R. and Fontecave, M. (1997) Proc. Natl Acad. Sci. USA, 94, 475-478.
10. Komori, K., Fujita, N., Ichiyanagi, K., Shinagawa, H., Morikawa, K. and Ishino, Y. (1999) Nucleic Acids Res., 27, 4167-4174.
11. Kim, J., Zwieb, C., Wu, C. and Adhya, S. (1989) Gene, 85, 15-23.
12. Pohler, J.R.G., Norman, D.G., Bramham, J., Bianchi, M.E. and Lilley, D.M.J. (1998) EMBO J., 17, 817-826.
13. Gimble, F.S. and Wang, J. (1996) J. Mol. Biol., 263, 163-180.
14. Wende, W., Grindl, W., Christ, F., Pingoud, A. and Pingoud, V. (1996) Nucleic Acids Res., 24, 4123-4132.
15. Thompson, J.F. and Landy, A. (1988) Nucleic Acids Res., 20, 9687-9705.
16. Gimble, F.S. and Stephens, B.W. (1995) J. Biol. Chem., 270, 5849-5856.
17. Luisi, B. (1995) In Lilley, D.M.J. (ed.), DNA-Protein: Structural Interactions. IRL Press, Oxford, UK, pp. 1-48.
18. Travers, A.A. (1995) In Lilley, D.M.J. (ed.), DNA-Protein: Structural Interactions. IRL Press, Oxford, UK, pp. 49-75.
19. Kim, Y., Grable, J.G., Love, R., Greene, P.J. and Rosenberg, J.M. (1990) Science, 249, 1307-1309.
20. Winkler, F.K., Banner, D.W., Oefner, C., Tsernoglou, D., Brown, R.S., Heathman, S.P., Bryan, R.K., Martin, P.D., Petratros, K. and Wilson, K.S. (1993) EMBO J., 12, 1781-1795.
21. Mueller, J.E., Smith, D., Bryk, M. and Belfort, M. (1995) EMBO J., 14, 5724-5735.
22. Loizos, N., Silva, G.H. and Belfort M. (1996) J. Mol. Biol., 255, 412-424.
23. Wittmayer, P.K. and Raines, R.T. (1996) Biochemistry, 35, 1076-1083.
24. Aggarwal, A.K. (1995) Curr. Opin. Struct. Biol., 5, 11-19.
25. He, Z., Crist, M., Yen, H.-C., Duan, X., Quiocho, F.A. and Gimble, F.S. (1998) J. Biol. Chem., 273, 4607-4615.
26. Pietrokovski, S. (1994) Protein Sci., 3, 2340-2350.
27. Perler, F.B., Olsen, G.J. and Adam, E. (1997) Nucleic Acids Res., 25, 1087-1093.
28. Perrin, A., Buckle, M. and Dujon, B. (1993) EMBO J., 12, 2939-2947.
29. Mueller, J.E., Smith, D. and Belfort, M. (1996) Genes Dev., 10, 2158-2166.
30. Dalgaard, J.Z., Klar, A.J., Moser, M.J., Holley, W.R., Chatterjee, A. and Mian, I.S. (1997) Nucleic Acids Res., 25, 4626-4638.
31. Seligman, L.M., Stephens, K.M., Savage, J.H. and Monnat, R.J.,Jr (1997) Genetics, 147, 1653-1664.