Remarkable stabilization of a psychrotrophic RNase HI by a combination of thermostabilizing mutations identified by the suppressor mutation method

Biochemistry

Volumn 47, Issue 31, 2008, Pages 8040-8047

  Tadokoro, Takashi ^a   Matsushita, Kyoko ^a   Abe, Yumi ^a   Rohman, Muhammad Saifur ^a   Koga, Yuichi ^a   Takano, Kazufumi ^a,b   Kanaya, Shigenori ^a  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

SHEWANELLA ONEIDENSIS;

ENZYMES;

RIBONUCLEASE H;

ARTICLE; BACTERIAL GROWTH; ENZYME ACTIVITY; ENZYME STABILITY; ESCHERICHIA COLI; GENETIC PROCEDURES; MUTAGENESIS; MUTATION; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHEWANELLA ONEIDENSIS; SUPPRESSOR MUTATION METHOD; TEMPERATURE SENSITIVITY;

BACTERIAL PROTEINS; CIRCULAR DICHROISM; ENZYME STABILITY; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN DENATURATION; RIBONUCLEASE H; SHEWANELLA; SUPPRESSION, GENETIC; TEMPERATURE; UREA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SHEWANELLA ONEIDENSIS MR-1;

EID: 48649109167     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi800246e     Document Type: Article

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