Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form

Journal of Biochemistry

Volumn 127, Issue 5, 2000, Pages 895-899

  Ohtani, Naoto ^a   Haruki, Mitsuru ^a   Muroya, Ayumu ^a   Morikawa, Masaaki ^a   Kanaya, Shigenori ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Amino acid sequence; Enzymatic activity; Escherichia coli; Overproduction; RNase H

Indexed keywords

BACTERIAL ENZYME; DNA HYBRID; MANGANESE; RIBONUCLEASE H; RIBONUCLEASE III; RNA;

AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SYNTHESIS; ESCHERICHIA COLI; GEL FILTRATION; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ITAYA;

EID: 0034038243     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022684     Document Type: Article

References (22)

1. Crouch, R.J. and Dirksen, M.-L. (1982) Ribonucleases H in Nuclease (Linn, S.M. and Roberts, R.J., eds.) pp. 211-241, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
2. Ohtani, N., Haruki, M., Morikawa, M., and Kanaya, S. (1999) Molecular diversities of RNases H. J. Biosci. Bioeng. 88, 12-19
3. Bellgard, M.I. and Gojobori, T. (1999) Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences. FEBS Lett. 445, 6-8
4. Zhang, Y.-B., Ayalew, S., and Lackes, S.A. (1997) The rnhB gene encoding RNase HII of Streptococcus pneumoniae and evidence of conserved motifs in eucaryotic genes. J. Bacteriol. 179, 3828-3836
5. Itaya, M. (1990) Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene. Proc. Natl. Acad. Sci. USA 87, 8587-8591
6. 2-dependent RNase HIII from Bacillus subtilis: Classification of RNases H into three families. Biochemistry 38, 605-618
7. Haruki, M., Hayashi, K., Kochi, T., Muroya, A., Koga, Y., Morikawa, M., Imanaka, T., and Kanaya, S. (1998) Gene cloning and characterization of recombinant ribonuclease HII from a hyperthermophilic archaeon. J. Bacteriol. 180, 6207-6214
8. Kanaya, S. and Crouch, R.J. (1983) DNA sequence of the gene coding for Escherichia coli ribonuclease H. J. Biol. Chem. 258, 1276-1281
9. Crouch, R.J. (1990) Ribonuclease H: from discovery to 3D structure. New Biol. 2, 771-777
10. Hostomsky, Z., Hostomska, Z., and Matthews, D.A. (1993) Ribonucleases H in Nucleases, 2nd ed. (Linn, S.M. and Roberts, R.J., eds.) pp. 341-376, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
11. Kanaya, S. (1998) Enzymatic activity and protein stability of E. coli ribonuclease HI in Ribonucleases H (Crouch, R.J. and Toulme, J.J., eds.) pp. 1-38, INSERM, Paris
12. Kanaya, S., Kohara, A., Miyagawa, M., Matsuzaki, T., Morikawa, K., and Ikehara, M. (1989) Overproduction and preliminary crystallographic study of ribonuclease H from Escherichia coli. J. Biol. Chem. 264, 11546-11549
13. Itaya, M. and Crouch, R.J. (1991) A combination of RNase H (rnh) and recBCD or sbcB mutations in Escherichia coli K12 adversely affects growth. Mol. Gen. Genet. 227, 424-432
14. Itaya, M., Omori, A., Kanaya, S., Crouch, R.J., Tanaka, T., and Kondo, K. (1999) Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. J. Bacteriol. 181, 2118-2123
15. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
16. Goodwin, T.W. and Morton, R.A. (1946) The spectrophotometric determination of tyrosine and tryptophan in proteins Biochem. J. 40, 628-632
17. Kanaya, S., Katsuda, C., Kimura, S., Nakai, T., Kitakuni, E., Nakamura, H., Katayanagi, K., Morikawa, K., and Ikehara, M. (1991) Stabilization of Escherichia coli ribonuclease H by introduction of an artificial disulfide bond. J. Biol. Chem. 266, 6038-6044
18. Blattner, F.R. et al. (1997) The complete genome sequence of Escherichia coli K-12. Science 277, 1453-1474
19. Wu, C.S.C., Ikeda, K., and Yang, J.T. (1981) Ordered conformation of polypeptides and proteins in acidic dodecyl sulfate solution. Biochemistry 20, 566-570
20. Katayanagi, K., Miyagawa, M., Matsushima, M., Ishikawa, M., Kanaya, S., Ikehara, M., Matsuzaki, T., and Morikawa, K. (1990) Three-dimensional structure of ribonuclease H from E. coli. Nature 347, 306-309
21. Keck, J.L. and Marqusee, S. (1996) The putative substrate recognition loop of Escherichia coli ribonuclease H is not essential for activity. J. Biol. Chem. 271, 19883-19887
22. Keck, J.L., Goedken, E.R., and Marqusee, S. (1998) Activation/ attenuation model for RNase H. A one-metal mechanism with second-metal inhibition. J. Biol. Chem. 273, 34128-34133