Conformational stabilities of Escherichia coli RNase HI variants with a series of amino acid substitutions at a cavity within the hydrophobic core

Journal of Biological Chemistry

Volumn 272, Issue 30, 1997, Pages 18686-18693

  Akasako, Atsuko ^a   Haruki, Mitsuru ^a,b   Oobatake, Motohisa ^a   Kanaya, Shigenori ^a,b  

^a BIOMOL ENG RESEARCH INSTITUTE   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ENZYME VARIANT; METHYL GROUP; MUTANT PROTEIN; RIBONUCLEASE H; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STABILITY; ESCHERICHIA COLI; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN STRUCTURE; THERMOSTABILITY; ULTRAVIOLET SPECTROSCOPY;

AMINO ACIDS; BINDING SITES; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN DENATURATION; RIBONUCLEASE H, CALF THYMUS;

ESCHERICHIA COLI;

EID: 0030802904     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.272.30.18686     Document Type: Article

References (60)

1. Karpusas, M., Baase, W. A., Matsumura, M. & Matthews, B. W. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 8237-8241
2. Kellis, J. T., Jr., Nyberg, K. & Fersht, A. R. (1989) Biochemistry 28, 4914-4922
3. Sandberg, W. S. & Terwilliger, T. C. (1989) Science 245, 54-57
4. Sandberg, W. S. & Terwilliger, T. C. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 1706-1710
5. Shortle, D., Stites, W. E. & Meeker, A. K. (1990) Biochemistry 29, 8033-8041
6. Dao-pin, S., Anderson, D. E., Baase, W. A., Dahlquist, F. W. & Matthews, B. W. (1991) Biochemistry 30, 11521-11529
7. Lim, W. A., Farruggio, D. C. & Sauer, R. T. (1992) Biochemistry 31, 4324-4333
8. Eijsink, V. G. H., Dijkstra, B. W., Vriend, G., van der Zee, J. R., Veltman, O. R., van der Vinne, B., van den Burg, B., Kempe, S. & Venema, G. (1992) Protein Eng. 5, 421-426
9. Eriksson, A. E., Baase, W. A., Zhang, X.-J., Heinz, D. W., Blaber, M., Baldwin, E. P. & Matthews, B. W. (1992) Science 255, 178-183
10. Wilson, K. P., Malcolm, B. A. & Matthews, B. W. (1992) J. Biol. Chem. 267, 10842-10849
11. Hurley, J. H., Baase, W. A. & Matthews, B. W. (1992) J. Mol. Biol. 224, 1143-1159
12. Varadarajan, R. & Richards, F. M. (1992) Biochemistry 31, 12315-12327
13. Jackson, S. E., Moracci, M., elMasry, N., Johnson, C. M. & Fersht, A. R. (1993) Biochemistry 32, 11259-11269
14. Ishikawa, K., Nakamura, H., Morikawa, K. & Kanaya, S. (1993b) Biochemistry 32, 6171-6178
15. Takano, K., Ogasahara, K., Kaneda, H., Yamagata, Y., Fujii, S., Kanaya, E., Kikuchi, M., Oobatake, M. & Yutani, K. (1995) J. Mol. Biol. 254, 62-76
16. Baldwin, E. P., Hajiseyedjavadi, O., Baase, W. A. & Matthews, B. W. (1993) Science 262, 1715-1718
17. Baldwin, E., Xu. J., Hajiseyedjavadi, O., Baase, W. A. & Matthews, B. W. (1996) J. Mol. Biol. 259, 542-559
18. Gassner, N. C., Baase, W. A. & Matthews, B. W. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 12155-12158
19. Buckle, A. M., Henrick, K. & Fersht, A. R. (1993) J. Mol. Biol. 234, 847-860
20. Buckle, A. M., Cramer, P. & Fersht, A. R. (1996) Biochemistry 35, 4298-4305
21. Zhang, H., Skinner, M. M., Sandberg, W. S., Wang, A. H.-J. & Terwilliger, T. C. (1996) J. Mol. Biol. 259, 148-159
22. Lim, W. A. & Sauer, R. T. (1989) Nature 339, 31-36
23. Lim, W. A. & Sauer, R. T. (1991) J. Mol. Biol. 219, 359-376
24. Axe, D. D., Foster, N. W. & Fersht, A. R. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 5590-5594
25. Lee, C. & Levitt, M. (1991) Nature 352, 448-451
26. Sneddon, S. F. & Tobias, D. J. (1992) Biochemistry 31, 2842-2846
27. van Gunsteren, W. F. & Mark, A. E. (1992) J. Mol. Biol. 227, 389-395
28. Sun, S., Brem, R., Chan, H. S. & Dill, K. A. (1995) Protein Eng. 8, 1205-1213
29. Zhang, L. & Hermans, J. (1996) Proteins Struct. Funct. Genet. 24, 433-438
30. Pace, C. N. (1992) J. Mol. Biol. 226, 29-35
31. Connolly, M. L. (1986) Int. J. Pept. Protein Res. 28, 360-363
32. Rashin, A. A., Iofin, M. & Honig, B. (1986) Biochemistry 25, 3619-3625
33. Williams, M., Goodfellow, J. M. & Thornton, J. M. (1994) Protein Sci. 3, 1224-1235
34. Hubbard, S. J., Gross, K.-H. & Argos, P. (1994) Protein Eng. 7, 613-626
35. Kanaya, S., Katsuda, C., Kimura, S., Nakai, T., Kitakuni, E., Nakamura, H., Katayanagi, K., Morikawa, K. & Ikehara, M. (1991) J. Biol. Chem. 266, 6038-6044
36. Kanaya, S., Oobatake, M., Nakamura, H. & Ikehara, M. (1993) J. Biatechnol. 28, 117-136
37. Kimura, S., Oda, Y., Nakai, T., Katayanagi, K., Kitakuni, E., Nakai, C., Nakamura, H., Ikehara, M. & Kanaya, S. (1992a) Eur. J. Biochem. 206, 337-343
38. Kimura, S., Nakamura, H., Hashimoto, T., Oobatake, M. & Kanaya, S. (1992b) J. Biol. Chem. 267, 21536-21542
39. Kimura, S., Kanaya, S. & Nakamura, H. (1992c) J. Biol. Chem. 267, 22014-22017
40. Ishikawa, K., Kimura, S., Kanaya, S., Morikawa, K. & Nakamura, H. (1993a) Protein Eng. 6, 85-91
41. Ishikawa, K., Nakamura, H., Morikawa, K. Kimura, S. & Kanaya, S. (1993c) Biochemistry 32, 7136-7142
42. Haruki, M., Noguchi, E., Akasako, A., Oobatake, M., Itaya, M. & Kanaya, S. (1994) J. Biol. Chem. 269, 26904-26911
43. Akasako, A., Haruki, M., Oobatake, M. & Kanaya, S. (1995) Biochemistry 34, 8115-8122
44. Kanaya, E. & Kanaya, S. (1995) J. Biol. Chem. 270, 19853-19860
45. Yamasaki, K., Ogasahara, K., Yutani, K., Oobatake, M. & Kanaya, S. (1995) Biochemistry 34, 16552-16562
46. Katayanagi, K., Miyagawa, M., Matsushima, M., Ishikawa, M., Kanaya, S., Nakamura, H., Ikehara, M., Matsuzaki, T. & Morikawa, K. (1992) J. Mol. Biol. 223, 1029-1052
47. Miller, J. H. (1972) in Experiments in Molecular Genetics (Miller, J. H., ed) pp. 433, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
48. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467
49. Kanaya, S., Kimura, S., Katsuda, C. & Ikehara, M. (1990) Biochem. J. 271, 59-66
50. Laemmli, U. K. (1970) Nature 227, 680-685
51. Becktel, W. J. & Schellman, J. A. (1987) Biopolymers 26, 1859-1877
52. Yutani, K., Ogasahara, K., Tsujita, T. & Sugino, Y. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 4441-4444
53. Matsumura, M., Becktel, W. J. & Matthews, B. W. (1988) Nature 334, 406-410
54. Barlow, D. J. & Thornton, J. M. (1988) J. Mol. Biol. 201, 601-619
55. Morikami, K., Nakai, T., Kidera, A., Saito, M. & Nakamura, H. (1992) Comput. Chem. 16, 243-248
56. Sauer, U. H., Dao-pin, S. & Matthews, B. W. (1992) J. Biol. Chem. 267, 2393-2399
57. Gray, T. M., Arnoys, E. J., Blankespoor, S., Born, T., Jagar, R., Everman, R., Plowman, D., Stair, A. & Zhang, D. (1996) Protein Sci. 5, 742-751
58. Kanaya, S., Oobatake, M. & Liu, Y. (1996) J. Biol. Chem. 271, 32729-32736
59. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950
60. Fauchere, J.-L. & Pliska, V. (1983) Ear. J. Med. Chem. 18, 369-375