Efficacy of three different laser wavelengths for in vitro wound healing

Photodermatology Photoimmunology and Photomedicine

Volumn 24, Issue 4, 2008, Pages 199-210

  Hawkins Evans, Denise ^a   Abrahamse, Heidi ^a  

^a UNIVERSITY OF JOHANNESBURG   (South Africa)

Author keywords

830 nm diode; Biological responses; Helium neon; Nd:YAG; Phototherapy; Wound

Indexed keywords

ADENOSINE TRIPHOSPHATE; ALKALINE PHOSPHATASE; CASPASE 3; CASPASE 7; INTERLEUKIN 6;

APOPTOSIS; ARTICLE; CELL COMMUNICATION; CELL MIGRATION; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CELLULAR IMMUNITY; CLINICAL EFFECTIVENESS; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DIODE LASER; DNA DAMAGE; ENZYME ACTIVITY; HELIUM NEON LASER; HUMAN; HUMAN CELL; IN VITRO STUDY; LOW LEVEL LASER THERAPY; NEODYMIUM LASER; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIATION RESPONSE; SKIN FIBROBLAST; SPECTRAL SENSITIVITY; TREATMENT DURATION; WOUND HEALING;

ALKALINE PHOSPHATASE; APOPTOSIS; CELL COMMUNICATION; CELL MOVEMENT; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; DNA DAMAGE; FIBROBLAST GROWTH FACTOR 2; FIBROBLASTS; HUMANS; INTERLEUKIN-6; LASERS, GAS; LASERS, SEMICONDUCTOR; LASERS, SOLID-STATE; LUMINESCENT MEASUREMENTS; RADIATION DOSAGE; STATISTICS, NONPARAMETRIC; WOUND HEALING;

EID: 48449094497     PISSN: 09054383     EISSN: 16000781     Source Type: Journal    
DOI: 10.1111/j.1600-0781.2008.00362.x     Document Type: Article

References (48)

1. Yu W, Naim JO, Lanzafame RJ. Effects of photostimulation on wound healing in diabetic mice. Lasers Surg Med 1997; 20: 56-63.
2. Schindl A, Schindl M, Pernerstorfer-Schon H, Schindl L. Low intensity laser therapy: A review. J Invest Med 2000; 48: 312-326.
3. Basford JR. Low intensity laser therapy: Still not an established clinical tool. Lasers Surg Med 1995; 16: 331-342.
4. Loevschall H, Arenholt-Bindslev D. Effect of low level diode laser irradiation on human oral mucosa fibroblasts in vitro. Lasers Surg Med 1994; 14: 347-354.
5. Tunér J, Hode L. Laser therapy -clinical practice and scientific background. Chapter 1. Some basic laser physics. Grängesberg, Sweden: Prima Books AB, 2002: 12, 21, 22 (ISBN: 91-631-1344-9).
6. Whittaker P, Patterson MJ. Ventricular remodelling after acute myocardial infarction: Effect of low-intensity laser irradiation. Lasers Surg Med 2000; 27: 29-38.
7. Karu TI. Molecular mechanisms of therapeutic effect of low intensity laser irradiation. Laser Life Sci 1988; 2: 53-74.
8. Almeida-Lopes L, Rigau J, Zângaro RA, Guidugli-Neto J, Jaeger MM. Comparison of the low level laser therapy effects on cultured human gingival fibroblast proliferation using different irradiance and same fluence. Lasers Surg Med 2001; 29: 179-184.
9. Pereira AN, Eduardo C de P, Matson E, Marques MM. Effect of low power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Laser Surg Med 2002; 31: 263-267.
10. Azevedo LH, de Paula Eduardo F, Moreira MS, de Paula Eduardo C, Marques MM. Influence of different power densities of LILT on cultured human fibroblast growth: A pilot study. Lasers Med Sci 2006; 21: 86-89.
11. Colver GB, Priestley GC. Failure of helium-neon laser to affect components of wound healing in vitro. Br J Dermatol 1989; 121: 179-186.
12. Hawkins D, Abrahamse H. The role of fluence in cell viability, proliferation and membrane integrity of wounded human skin fibroblasts following helium-neon laser irradiation. Lasers Surg Med 2006; 38: 74-83.
13. Hawkins D, Abrahamse H. Biological effects of helium-neon (632.8 nm) laser irradiation on normal and wounded human skin fibroblasts. J Photomed Laser Surg 2005; 23: 251-259.
14. Hawkins D, Abrahamse H. The release of interleukin-6 after Low Level Laser Therapy (LLLT) and the effect on migration and proliferation of human skin fibroblasts-An in vitro study. Med Technol SA 2004; 18: 11-15.
15. Hawkins D, Abrahamse H. Effect of multiple exposures of low level laser therapy on the cellular responses of wounded human skin fibroblasts. J Photomed Laser Surg 2006; 24: 715-724.
16. Hawkins D, Abrahamse H. How long after laser irradiation should cellular responses be measured to determine the laser effect?-An in vitro study. J Laser Appl 2007; 19: 74-83.
17. Hawkins D, Abrahamse H. Changes in cell viability of wounded fibroblasts following laser irradiation in broad-spectrum or infrared light. Laser Chem 2007; 2007, 10pp.
18. Abrahamse H, Hawkins D, Houreld N. Effect of wavelength and fluence on morphology, cellular and genetic integrity of diabetic wounded human skin fibroblasts. Photonics West, Proceedings of SPIE International Society for Optical Engineering. Photonics West Conference: Mechanisms for Low-Light therapy, San Jose Convention Centre, 2006: 6140-07.
19. 2 stimulates proliferation and expression of interleukin-6 in diabetic wounded fibroblast cells. Diabetes Technol Ther 2007; 9: 451-459.
20. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbour, NY: Cold Spring Laboratory, 1989.
21. Yarrow JC, Perlman ZE, Westwood NJ, Mitchison TJ. A high throughput cell migration assay using scratch wound healing, a comparison of imaged based readout methods. Biomed Cent Biotechnol 2004; 4: 21.
22. Rigau J, Sun C, Trelles MA, Berns M. Effects of the 633 nm laser on the behavior and morphology of primary fibroblasts in culture. In: Karu T, Young A, eds. Effects of low power light on biological systems, Barcelona, Spain. Progress in Biomedical Optics. Bellingham, WA, USA: SPIE, 1995; 38-42.
23. Cha D, O'Brien P, O'Toole EA, Woodley DT, Hudson LG. Enhanced modulation of keratinocyte motility by TGFα relative to EGF. J Investig Dermatol 1996; 106: 590-597.
24. Kornyei Z, Czorok A, Vicsek T, Madarasz E. Proliferative and migratory responses of astrocytes to in vitro injury. J Neurosci Res 2000; 61: 421-429.
25. Saga Y, Mizukami H, Takei Y, Ozawa K, Suzuki M. Suppression of cell migration in ovarian cancer cells mediated by PTEN overexpression. Int J Oncol 2003; 23: 1109-1113.
26. Farooqui R, Fenteany G. Multiple rows of cells behind epithelial wound edge extend crytic lammellipodia to collectively drive cell sheet movement. J Cell Sci 2004; 118: 51-63.
27. Walker DC, Hill G, Wood SM, Smallwood RH, Southgate J. Agent-based computational modelling of wounded epithelial cell monolayers. IEEE Trans Nanobiosci 2004; 3: 153-163.
28. Pinheiro ALB, Nascimento SC, Vieira AL, et al. Effects of low level laser therapy on malignant cells: In vitro study. J Clin Laser Med Surg 2002; 20: 23-26.
29. Van Snick J. Interleukin-6: An overview. Ann Rev Immunol 1990; 8: 253-278.
30. Hirano T, Yasukawa K, Harada H, et al. Complementary DNA for a novel human interleukin (BSF02) that induces B lymphocytes to produce immunoglobulin. Nature 1996; 324: 73-76.
31. Nicholson DW, Thornberry NA. Caspases: Killer proteases. Trends Biochem Sci 1997; 22: 299-306.
32. Riss T, Moravec R, Beck M, Hannah R, Wilson K, Swanson R. CellTiter-Glo luminescent cell viability assay: Fast, sensitive and flexible. Promega Notes Magazine 2002; 81: 2-5.
33. Crounch SPM. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J Immunol Meth 1993; 160: 81.
34. Takamiya M, Saigusa K, Nakayashiki N, Aoki Y. Studies on mRNA expression of basic fibroblast growth factor in wound healing for wound age determination. Int J Legal Med 2003; 117: 46-50.
35. Vogt RF, Phillips DL, Henderson LO, Whitfield W, Spierto FW. Quantitative differences among various proteins as blocking agents for ELISA microtiter plates. J Immunol Methods 1987; 101: 43-50.
36. Abe T, Abe Y, Aida Y, Hara Y, Maeda K. Extracellular matrix regulates induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts. J Cell Physiol 2001; 189: 144-151.
37. Aplaslan G, Nakajima T, Takano Y. Extracellular alkaline phosphatase activity as a possible marker for wound healing: A preliminary report. Oral Mazillofac Surg 1997; 55: 56-62.
38. Moravec R. Total cell quantitation using the CytoTox 96 ® non-radioactive cytotoxicity assay. Promega Notes 1994; 45: 11-12.
39. Collins AR. Measurement of oxidative DNA damage using the comet assay. In: Lunec J, Griffiths HR, eds. Measuring in vivo oxidativA damage: a practical approach. UK: John Wiley and Sons Ltd., 2000; 83-94.
40. Yarbro JW, Kennedy BJ, Barnum CP. Hydroxyurea inhibition of DNA synthesis in ascites tumour. Proc Natl Acad Sci 1965; 53: 1033-1035.
41. Karu T. Primary and secondary mechanisms of the action of monochromatic visible and near infrared radiation on cells. In: Karu T, ed. The science of low-power laser therapy; Vol. 33. Amsterdam, the Netherlands: Gordon and Breach Science Publishers, 1998; 33-34. (ISBN 90-5699-108-6).
42. Rippey FF. Illustrated lecture notes: General pathology. Chapter 3 cell injury. Johannesburg, South Africa: Witwatersrand University Press, 1994; 19-27. (ISBN 1 86814 24).
43. Kao B, Amikura Y, Honda E, Tomizuka Y, Hosaka Y. Novel model for evaluation of human skin injury. In: Kusano M, Shioda S, eds. New frontiers in regenerative medicine. Part 1. Japan: Springer, 2007; 33-36. (ISBN 978-4-431-38207-2).
44. Kao B, Kristen KM, Majaron B, Nelson JS. Novel model for evaluation of epidermal preservation and dermal collagen remodelling following photorejuvenation of human skin. Lasers Surg Med 2003; 32: 115-119.
45. Vaughan M, Ramrez RD, Brown SA, Yang JC, Wright WE, Shay JW. A reproducible laser-wounded skin equivalent model to study the effects of ageing in vitro. Rejuvenation Res 2004; 7: 99-110.
46. Karu TI, Kalyakov SF. Exact action spectra for cellular responses relevant to phototherapy. Photomed Laser Surg 2005; 23: 355-361.
47. Mognato M, Squizzato F, Faccin F, Zaghetto L, Corti L. Cell growth modulation of human cells irradiated in vitro with low level laser therapy. Photomed Laser Surg 2004; 22: 523-526.
48. Hamblin MR, Deidova TN. Mechanisms of low level light therapy. Progress in Biomedical Optics and Imaging, Mechanisms for Low-Light Therapy, 2006: Proc SPIE 6140, 614001-1-614001-11.