A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

BMC Biotechnology

Volumn 4, Issue , 2004, Pages

  Yarrow, Justin C ^a   Perlman, Zachary E ^a   Westwood, Nicholas J ^a,b   Mitchison, Timothy J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; DATA ACQUISITION; FLUORESCENCE; MICROSCOPIC EXAMINATION; MONOLAYERS; PERTURBATION TECHNIQUES; RNA; SUBSTRATES; TISSUE;

CELL DIVISION; CELL MIGRATION; CELL MONOLAYERS; TISSUE REORGANIZATION;

CELLS;

SMALL INTERFERING RNA; ACTIN; DNA;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIVISION; CELL MIGRATION; CELL STRUCTURE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; HIGH THROUGHPUT SCREENING; IMAGING SYSTEM; INTERMETHOD COMPARISON; NONHUMAN; PHENOTYPE; QUANTITATIVE ANALYSIS; SCREENING TEST; VALIDATION PROCESS; WOUND HEALING; ANIMAL; BIOASSAY; BIOLOGICAL MODEL; CELL MOTION; CERCOPITHECUS; COMPARATIVE STUDY; COMPUTER ASSISTED DIAGNOSIS; CYTOLOGY; KIDNEY; METABOLISM; METHODOLOGY; PHYSIOLOGY; STAINING;

ACTINS; ANIMALS; BIOLOGICAL ASSAY; CELL MOVEMENT; CERCOPITHECUS AETHIOPS; DNA; IMAGE INTERPRETATION, COMPUTER-ASSISTED; KIDNEY; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; STAINING AND LABELING; WOUND HEALING;

EID: 13244255307     PISSN: 14726750     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6750-4-21     Document Type: Article

References (25)

1. Wong MK, Gotlieb AI: The reorganization of microfilaments, centrosomes, and microtubules during in vitro small wound reendothelialization. J Cell Biol 1988, 107:1777-1783.
2. Coomber BL, Gotlieb AI: In vitro endothelial wound repair. Interaction of cell migration and proliferation. Arteriosclerosis 1990, 10:215-222.
3. Zahm JM, Kaplan H, Herard AL, Doriot F, Pierrot D, Somelette P, Puchelle E: Cell migration and proliferation during the in vitro wound repair of the respiratory epithelium. Cell Motil Cytoskeleton 1997, 37:33-43.
4. Lampugnani MG: Cell migration into a wounded area in vitro. Methods Mol Biol 1999, 96:177-182.
5. Lu KV, Jong KA, Rajasekaran AK, Cloughesy TF, Mischel PS: Upregulation of tissue inhibitor of metalloproteinases (TIMP)-2 promotes matrix metalloproteinase (MMP)-2 activation and cell invasion in a human glioblastoma cell line. Lab Invest 2004, 84:8-20.
6. Herren B, Garton KJ, Coats S, Bowen-Pope DF, Ross R, Raines EW: ADAM15 overexpression in NIH3T3 cells enhances cell-cell interactions. Exp Cell Res 2001, 271:152-160.
7. Huang C, Rajfur Z, Borchers C, Schaller MD, Jacobson K: JNK phosphorylates paxillin and regulates cell migration. Nature 2003, 424:219-223.
8. Mc Henry KT, Ankala SV, Ghosh AK, Fenteany G: A non-antibacterial oxazolidinone derivative that inhibits epithelial cell sheet migration. Chembiochem 2002, 3:1105-1111.
9. Vogt A, Pestell KE, Day BW, Lazo JS, Wipf P: The antisignaling agent SC-alpha alpha delta 9, 4-(benzyl-(2-[(2,5-diphenyloxazole-4-carbonyl)amino]ethyl)carbamoyl)- 2-decanoylaminobutyric acid, is a structurally unique phospholipid analogue with phospholipase C inhibitory activity. Mol Cancer Ther 2002, 1:885-892.
10. Klein-Soyer C, Cazenave JP, Herbert JM, Maffrand JP: SR 25989 inhibits healing of a mechanical wound of confluent human saphenous vein endothelial cells which is modulated by standard heparin and growth factors. J Cell Physiol 1994, 160:316-322.
11. Fischer EG, Stingl A, Kirkpatrick CJ: Migration assay for endothelial cells in multiwells. Application to studies on the effect of opioids. J Immunol Methods 1990, 128:235-239.
12. Nobes CD, Hall A: Rho GTPases control polarity, protrusion, and adhesion during cell movement. J Cell Biol 1999, 144:1235-1244.
13. Etienne-Manneville S, Hall A: Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzeta. Cell 2001, 106:489-498.
14. Fenteany G, Janmey PA, Stossel TP: Signaling pathways and cell mechanics involved in wound closure by epithelial cell sheets. Curr Biol 2000, 10:831-838.
15. Sablina AA, Chumakov PM, Kopnin BP: Tumor suppressor p53 and its homologue p73alpha affect cell migration. J Biol Chem 2003, 278:27362-27371.
16. Yvon AM, Walker JW, Danowski B, Fagerstrom C, Khodjakov A, Wadsworth P: Centrosome reorientation in wound-edge cells is cell type specific. Mol Biol Cell 2002, 13:1871-1880.
17. Magdalena J, Millard TH, Machesky LM: Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishment. J Cell Sci 2003, 116:743-756.
18. Magdalena J, Millard TH, Etienne-Manneville S, Launay S, Warwick HK, Machesky LM: Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound models. Mol Biol Cell 2003, 14:670-684.
19. Philp D, Huff T, Gho YS, Hannappel E, Kleinman HK: The actin binding site on thymosin beta4 promotes angiogenesis. Faseb J 2003, 17:2103-2105.
20. Moon EJ, Lee YM, Lee OH, Lee MJ, Lee SK, Chung MH, Park YI, Sung CK, Choi JS, Kim KW: A novel angiogenic factor derived from Aloe vera gel: beta-sitosterol, a plant sterol. Angiogenesis 1999, 3:117-123.
21. Ma PC, Kijima T, Maulik G, Fox EA, Sattler M, Griffin JD, Johnson BE, Salgia R: c-MET mutational analysis in small cell lung cancer: novel juxtamembrane domain mutations regulating cytoskeletal functions. Cancer Res 2003, 63:6272-6281.
22. Chung RS, Adlard PA, Dittmann J, Vickers JC, Chuah MI, West AK: Neuron-glia communication: metallothionein expression is specifically up-regulated by astrocytes in response to neuronal injury. J Neurochem 2004, 88:454-461.
23. Morgan LW, Wieslander A, Davies M, Horiuchi T, Ohta Y, Beavis MJ, Craig KJ, Williams JD, Topley N: Glucose degradation products (GDP) retard remesothelialization independently of D-glucose concentration. Kidney Int 2003, 64:1854-1866.
24. Saga Y, Mizukami H, Takei Y, Ozawa K, Suzuki M: Suppression of cell migration in ovarian cancer cells mediated by PTEN overexpression. Int J Oncol 2003, 23:1109-1113.
25. Ramm P: Imaging systems in assay screening. Drug Discov Today 1999, 4:401-410.