Solubilization at high pH results in improved recovery of proteins from inclusion bodies of E. coli

Journal of Chemical Technology and Biotechnology

Volumn 83, Issue 8, 2008, Pages 1126-1134

  Singh, Surinder M ^a   Upadhyay, Arun K ^a   Panda, Amulya K ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Inclusion bodies; Mild solubilization; Protein recovery; Pulsatile refolding

Indexed keywords

AGGLOMERATION; CONCENTRATION (PROCESS); ENHANCED RECOVERY; ESCHERICHIA COLI; OXYGEN; SECONDARY RECOVERY; SOLUBILITY; WATER POLLUTION;

ALDOLASE; BIOACTIVE PROTEINS; CHAOTROPES; E. COLI; ENOLASE; H IGH CONCENTRATIONS; HIGH PH; INCLUSION BODIES; MILD SOLUBILIZATION; PROTEIN RECOVERY; PULSATILE REFOLDING; REFOLDING; SECONDARY STRUCTURES; SUPER OXIDE DISMUTASE;

PROTEINS;

ENOLASE; FRUCTOSE BISPHOSPHATE ALDOLASE; GROWTH HORMONE; HUMAN GROWTH HORMONE; POLYKETIDE SYNTHASE; PYRUVATE KINASE; SUPEROXIDE DISMUTASE; UREA;

ALKALINITY; ARTICLE; CELL INCLUSION; CYTOLYSIS; ENRICHMENT CULTURE; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; PH; PROTEIN AGGREGATION; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN PURIFICATION; SOLUBILIZATION;

EID: 48449087975     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.1945     Document Type: Article

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