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Volumn 130, Issue 30, 2008, Pages 9650-9651

Identification of strong DNA binding motifs for bleomycin

Author keywords

[No Author keywords available]

Indexed keywords

BLEOMYCIN;

EID: 48249107066     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja802905g     Document Type: Article
Times cited : (37)

References (28)
  • 19
    • 0036000112 scopus 로고    scopus 로고
    • The resin-bound BLM exhibits DNA cleavage properties quite similar to the free species. See
    • The resin-bound BLM exhibits DNA cleavage properties quite similar to the free species. See: Tao, Z.-F.; Leitheiser, C. J.; Smith, K. L.; Hashimoto, S.; Hecht, S. M. Bioconjugate Chem. 2002, 13, 426.
    • (2002) Bioconjugate Chem , vol.13 , pp. 426
    • Tao, Z.-F.1    Leitheiser, C.J.2    Smith, K.L.3    Hashimoto, S.4    Hecht, S.M.5
  • 22
    • 48249134110 scopus 로고    scopus 로고
    • The extent of DNA cleavage by different BLMs (PAGE assay) versus intensity of fluorescence enhancement obtained with the fluorescent hairpin DNA indicated very similar cleavage efficiencies in both assay systems
    • The extent of DNA cleavage by different BLMs (PAGE assay) versus intensity of fluorescence enhancement obtained with the fluorescent hairpin DNA indicated very similar cleavage efficiencies in both assay systems.
  • 23
    • 48249115714 scopus 로고    scopus 로고
    • 2+ (100%) (Figure 1).
    • 2+ (100%) (Figure 1).
  • 24
    • 48249128074 scopus 로고    scopus 로고
    • While the initial experiments were carried out using hairpin DNAs isolated as outlined in Scheme 1, detailed characterization employed chemically synthesized DNAs prepared to verify the initial observations
    • While the initial experiments were carried out using hairpin DNAs isolated as outlined in Scheme 1, detailed characterization employed chemically synthesized DNAs prepared to verify the initial observations.
  • 25
    • 48249139163 scopus 로고    scopus 로고
    • 19,20 This method requires the assumption that bithiazole fluorescence quenching bears the same proportionality to DNA binding for all DNAs. The rather different efficiencies of quenching of bithiazole fluorescence by DNAs shown functionally to bind BLM with comparable affinities (Figure 1) suggests that this assumption is suspect.
    • 19,20 This method requires the assumption that bithiazole fluorescence quenching bears the same proportionality to DNA binding for all DNAs. The rather different efficiencies of quenching of bithiazole fluorescence by DNAs shown functionally to bind BLM with comparable affinities (Figure 1) suggests that this assumption is suspect.
  • 27
    • 48249141537 scopus 로고    scopus 로고
    • The apparent equilibrium constant is defined as 1/(C, 1/nK(D0)(B, 1/n(D0, where n, number of bound BLM/DNA nt, B, free BLM concn, C, bound BLM concn, D0, total DNA concn, B) and (C) were obtained from the fluorescence quenching caused by association between BLM and hairpin DNA and calculated from (B, F, Fc, Fb, Fc, B0) and C, Fb, F, Fb, Fc, B0, where F, quenched fluorescence intensity between BLM and hairpin DNA, Fb, fluorescence intensity of free BLM, Fc, fluorescence intensity of bound BLM, B0, total BLM concn
    • 0) = total BLM concn.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.