Validation of reference genes for qPCR studies on Caco-2 cell differentiation

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 69, Issue 3, 2008, Pages 1187-1192

  Piana, Claudia ^a   Wirth, Michael ^a   Gerbes, Stefan ^b   Viernstein, Helmut ^a   Gabor, Franz ^a   Toegel, Stefan ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b INM LEIBNIZ INSTITUTE FOR NEW MATERIALS   (Germany)

Author keywords

Caco 2; Cell surface interaction; Differentiation; geNorm; Normalization; qPCR; Reference genes

Indexed keywords

ALKALINE PHOSPHATASE; BETA 2 MICROGLOBULIN; BETA ACTIN; GENE PRODUCT; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; SUCCINATE DEHYDROGENASE; TRYPTOPHAN HYDROXYLASE; TYROSINE 3 MONOOXYGENASE;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL STRAIN CACO 2; CITRIC ACID CYCLE; CONTROLLED STUDY; CYTOSKELETON; DNA SYNTHESIS; GENE EXPRESSION; GENE FUNCTION; GLUCONEOGENESIS; GLYCOLYSIS; HUMAN; HUMAN CELL; MAJOR HISTOCOMPATIBILITY COMPLEX; POLYMERASE CHAIN REACTION; RNA ISOLATION; SIGNAL TRANSDUCTION;

CACO-2 CELLS; DATA INTERPRETATION, STATISTICAL; GENE EXPRESSION; GENES; HUMANS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SOFTWARE; TRANSCRIPTION, GENETIC;

EID: 48149099921     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2008.03.008     Document Type: Article

References (10)

1. Delie F., and Rubas W. A human colonic cell line sharing similarities with enterocytes as a model to examine oral absorption: advantages and limitations of the Caco-2 model. Crit. Rev. Ther. Drug Carrier Syst. 14 (1997) 221-286
2. Basson M.D., Turowski G., and Emenaker N.J. Regulation of human (Caco-2) intestinal epithelial cell differentiation by extracellular matrix proteins. Exp. Cell Res. 225 (1996) 301-305
3. Piana C., Guell I., Gerbes S., Gerdes R., Mills C., Samitier J., Wirth M., and Gabor F. Influence of surface modification on vitality and differentiation of Caco-2 cells. Differentiation 75 (2007) 308-317
4. Bustin S.A. A-Z of Quantitative PCR (2004), IUL Press, San Diego
5. Huggett J., Dheda K., Bustin S., and Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 6 (2005) 279-284
6. Dheda K., Huggett J.F., Bustin S.A., Johnson M.A., Rook G., and Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 37 (2004) 112-119
7. Dheda K., Huggett J.F., Chang J.S., Kim L.U., Bustin S.A., Johnson M.A., Rook G.A.W., and Zumla A. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal. Biochem. 344 (2005) 141-143
8. Toegel S., Huang W., Piana C., Unger F.M., Wirth M., Goldring M.B., Gabor F., and Viernstein H. Selection of reliable reference genes for qPCR studies on chondroprotective action. BMC Mol. Biol. 8 (2007) 13
9. Vandesompele J., DePreter K., Pattyn F., Poppe B., VanRoy N., DePaepe A., and Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3 (2002) 34
10. Olsen L., Bressendorff S., Troelsen J.T., and Olsen J. Differentiation-dependent activation of the human intestinal alkaline phosphatase promoter by HNF-4 in intestinal cells. Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2005) G220-G226