Adenosine A2A receptor dynamics studied with the novel fluorescent agonist Alexa488-APEC

European Journal of Pharmacology

Volumn 590, Issue 1-3, 2008, Pages 36-42

  Brand, Frank ^a   Klutz, Athena M ^b   Jacobson, Kenneth A ^b   Fredholm, Bertil B ^a   Schulte, Gunnar ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

Clathrin; CREB; Cyclic AMP; Endocytosis; G protein coupled receptor; Nucleoside; Rab5

Indexed keywords

4 [2 [7 AMINO 2 (2 FURYL) 1,2,4 TRIAZOLO[2,3 A][1,3,5]TRIAZIN 5 YLAMINO]ETHYL]PHENOL; ADENOSINE A1 RECEPTOR; ADENOSINE A2A RECEPTOR; ADENOSINE A2A RECEPTOR AGONIST; ALEXA 488; CLATHRIN; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; FLUORESCENT DYE; FORSKOLIN; N [2 (4 BROMOCINNAMYLAMINO)ETHYL] 5 ISOQUINOLINESULFONAMIDE; N6 (4 AMINO 3 IODOBENZYL)ADENOSINE 5' N ETHYLCARBOXAMIDOADENOSINE; RAB PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG RECEPTOR BINDING; DRUG SYNTHESIS; ENDOSOME; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INTERNALIZATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION;

ADENOSINE; ADENOSINE-5'-(N-ETHYLCARBOXAMIDE); ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; CYCLIC AMP; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; DOSE-RESPONSE RELATIONSHIP, DRUG; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FLUORESCENT DYES; HUMANS; RADIOLIGAND ASSAY; RECEPTOR, ADENOSINE A2A;

EID: 48049102996     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejphar.2008.05.036     Document Type: Article

References (46)

1. Ahn S., Shenoy S.K., Wei H., and Lefkowitz R.J. Differential kinetic and spatial patterns of beta-arrestin and G protein-mediated ERK activation by the angiotensin II receptor. J. Biol. Chem. 279 (2004) 35518-35525
2. 1-adenosine receptor-antagonist complexes in single living cells. Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 4673-4678
3. Bryja V., Gradl D., Schambony A., Arenas E., and Schulte G. Beta-arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo. Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 6690-6695
4. 2A receptor interacts with the actin-binding protein alpha-actinin. J. Biol. Chem. 278 (2003) 37545-37552
5. Conn P.M., Ulloa-Aguirre A., Ito J., and Janovick J.A. G protein-coupled receptor trafficking in health and disease: lessons learned to prepare for therapeutic mutant rescue in vivo. Pharmacol. Rev. 59 (2007) 225-250
6. Daly C.J., and McGrath J.C. Fluorescent ligands, antibodies, and proteins for the study of receptors. Pharmacol. Ther. 100 (2003) 101-118
7. Dewire S.M., Ahn S., Lefkowitz R.J., and Shenoy S.K. Beta-arrestins and cell signaling. Annu. Rev. Physiol. 69 (2007) 483-510
8. Drake M.T., Shenoy S.K., and Lefkowitz R.J. Trafficking of G protein-coupled receptors. Circ. Res. 99 (2006) 570-582
9. 3 adenosine receptors: consequences for agonist-stimulated translocation of arrestin3. Biochemistry 41 (2002) 14748-14761
10. Fredholm B.B., IJzerman A.P., Jacobson K.A., Klotz K.N., and Linden J. International Union of Pharmacology. XXV. Nomenclature and classification of adenosine receptors. Pharmacol. Rev. 53 (2001) 527-552
11. 2A receptors and therapeutic applications. Curr. Top. Med. Chem. 3 (2003) 413-426
12. Fredholm B.B., Chern Y., Franco R., and Sitkovsky M. Prog. Neurobiol. 83 (2007) 263-276
13. 2 receptor. Mol. Brain Res. 15 (1992) 62-66
14. Gao Z.G., Mamedova L.K., Chen P., and Jacobson K.A. 2-Substituted adenosine derivatives: affinity and efficacy at four subtypes of human adenosine receptors. Biochem. Pharmacol. 68 (2004) 1985-1993
15. Gurevich V.V., Pals-Rylaarsdam R., Benovic J.L., Hosey M.M., and Onorato J.J. Agonist-receptor-arrestin, an alternative ternary complex with high agonist affinity. J. Biol. Chem. 272 (1997) 28849-28852
16. Heuser J.E., and Anderson R.G. Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation. J. Cell Biol. 108 (1989) 389-400
17. 2A adenosine receptors from rat striatum and rat pheochromocytoma PC12 cells: characterization with radioligand binding and by activation of adenylate cyclase. Mol. Pharmacol. 41 (1992) 352-359
18. 2 receptors. J. Biol. Chem. 277 (2002) 18091-18097
19. Hoffmann C., Gaietta G., Bünemann M., Adams S.R., Oberdorff-Maass S., Behr B., Vilardaga J.P., Tsien R.Y., Ellisman M.H., and Lohse M.J. A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells. Nat. Methods 2 (2005) 171-176
20. Huang Z.L., Urade Y., and Hayaishi O. Prostaglandins and adenosine in the regulation of sleep and wakefulness. Curr. Opin. Pharmacol. 7 (2007) 33-38
21. Jacobson K.A., and Gao Z.G. Adenosine receptors as therapeutic targets. Nat. Rev. Drug Discov. 5 (2006) 247-264
22. 2 adenosine receptors. J. Mol. Recognit. 2 (1989) 170-178
23. Khoa N.D., Postow M., Danielsson J., and Cronstein B.N. Tumor necrosis factor-alpha prevents desensitization of Gas-coupled receptors by regulating GRK2 association with the plasma membrane. Mol. Pharmacol. 69 (2006) 1311-1319
24. Klotz K.N., Hessling J., Hegler J., Owman C., Kull B., Fredholm B.B., and Lohse M.J. Comparative pharmacology of human adenosine receptor subtypes - characterization of stably transfected receptors in CHO cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 357 (1998) 1-9
25. 2 receptors in Chinese hamster ovary cells co-transfected with the two receptors. Biochem. Pharmacol. 58 (1999) 1035-1045
26. 2a receptor. Nature 388 (1997) 674-678
27. Lin F.T., Krueger K.M., Kendall H.E., Daaka Y., Fredericks Z.L., Pitcher J.A., and Lefkowitz R.J. Clathrin-mediated endocytosis of the beta-adrenergic receptor is regulated by phosphorylation/dephosphorylation of beta-arrestin1. J. Biol. Chem. 272 (1997) 31051-31057
28. 2B adenosine receptor is mediated by a serine residue close to the COOH terminus. J. Biol. Chem. 276 (2001) 30199-30207
29. Middleton R.J., and Kellam B. Fluorophore-tagged GPCR ligands. Curr. Opin. Chem. Biol. 9 (2005) 517-525
30. 1-receptor agonists that allow quantification of ligand-receptor interactions in microdomains of single living cells. J. Med. Chem. 50 (2007) 782-793
31. 2A receptor. Mol. Pharmacol. 69 (2006) 1083-1094
32. Mundell S.J., and Kelly E. The effect of inhibitors of receptor internalization on the desensitization and resensitization of three Gs-coupled receptor responses. Br. J. Pharmacol. 125 (1998) 1594-1600
33. 2 receptor desensitization. Mol. Pharmacol. 51 (1997) 991-998
34. 2A adenosine receptors to agonist-induced desensitization. Br. J. Pharmacol. 125 (1998) 347-356
35. 2a adenosine receptor domain specifically responsible for mediating short-term desensitization. Biochemistry 36 (1997) 832-838
36. 2a adenosine receptor: delineation of multiple processes. Mol. Pharmacol. 45 (1994) 1082-1094
37. 2a adenosine receptor. Mol. Pharmacol. 48 (1995) 970-974
38. 3 adenosine receptor antagonists. J. Med. Chem. 48 (2005) 4910-4918
39. Pierce K.L., Maudsley S., Daaka Y., Luttrell L.M., and Lefkowitz R.J. Role of endocytosis in the activation of the extracellular signal-regulated kinase cascade by sequestering and nonsequestering G protein-coupled receptors. Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 1489-1494
40. 2a selective adenosine receptor antagonist. Br. J. Pharmacol. 115 (1995) 1096-1102
41. Roberts R.L., Barbieri M.A., Pryse K.M., Chua M., Morisaki J.H., and Stahl P.D. Endosome fusion in living cells overexpressing GFP-rab5. J. Cell Sci. 112 (1999) 3667-3675
42. 3 receptors expressed in Chinese hamster ovary cells all mediate the phosphorylation of extracellular-regulated kinase 1/2. Mol. Pharmacol. 58 (2000) 477-482
43. Schulte G., and Fredholm B.B. Signalling from adenosine receptors to mitogen-activated protein kinases. Cell. Signal. 15 (2003) 813-827
44. 2B receptor recruits divergent pathways to regulate ERK1/2 and p38. Exp. Cell Res. 290 (2003) 168-176
45. Wolfe B.L., and Trejo J. Clathrin-dependent mechanisms of G protein-coupled receptor endocytosis. Traffic 8 (2007) 462-470
46. Xu Z.Q., Zhang X., and Scott L. Regulation of G protein-coupled receptor trafficking. Acta Physiol. 190 (2007) 39-45