Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs

Genetics and Molecular Research

Volumn 7, Issue 2, 2008, Pages 284-294

  Jose A A F B V ^a   Gama, M A S ^b   Lanna, D D P ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b EMBRAPA CERRADOS   (Brazil)

Author keywords

Adipose tissue explants; Bioactive fatty acid; Conjugated linoleic acid; Lipogenesis; Swine

Indexed keywords

ANTIBIOTIC AGENT; CARBON 14; CONJUGATED LINOLEIC ACID; DEXAMETHASONE; FATTY ACID; FATTY ACID SYNTHASE; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GROWTH HORMONE; INSULIN; ISOPRENALINE; LINOLEIC ACID; MALATE DEHYDROGENASE (NADP); MESSENGER RNA; PHOSPHOGLUCONATE DEHYDROGENASE; POLYVINYL ALCOHOL;

ADIPOSE TISSUE; ANIMAL TISSUE; ARTICLE; ENZYME ACTIVITY; EXPLANT; GENE EXPRESSION; GROWTH; LIPID METABOLISM; LIPOGENESIS; LIPOLYSIS; MALE; NONHUMAN; SWINE;

SUIDAE; SUS;

EID: 47949121252     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/vol7-2gmr417     Document Type: Article

References (52)

1. Back DW, Goldman MJ, Fisch JE, Ochs RS, et al. (1986). The fatty acid synthase gene in avian liver. Two mRNAs are expressed and regulated in parallel by feeding, primarily at the level of transcription. J. Biol. Chem. 261: 4190-4197.
2. Bauman DE and Davis CL (1975). Regulation of lipid metabolism. In: Digestion and Metabolism in the Ruminant (Mcdonald IW and Warner ACI, eds.). Butterworths, London, 67-83.
3. Bauman DE, Brown RE and Davis CL (1970). Pathways of fatty acid synthesis and reducing equivalent generation in mammary gland of rat, sow, and cow. Arch. Biochem. Biophys. 140: 237-244.
4. Baumgard LH, Corl BA, Dwyer DA, Saebo A, et al. (2000). Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis. Am. J. Physiol. Regul. Integr. Comp. Physiol. 278: R179-R184.
5. Baumgard LH, Matitashvili E, Corl BA, Dwyer DA, et al. (2002). trans-10, cis-12 conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows. J. Dairy Sci. 85: 2155-2163.
6. Bee G (2000). Dietary conjugated linoleic acids alter adipose tissue and milk lipids of pregnant and lactating sows. J. Nutr. 130: 2292-2298.
7. Bissonauth V, Chouinard Y, Marin J, Leblanc N, et al. (2006). The effects of t10,c12 CLA isomer compared with c9,t11 CLA isomer on lipid metabolism and body composition in hamsters. J. Nutr. Biochem. 17: 597-603.
8. Brown JM and McIntosh MK (2003). Conjugated linoleic acid in humans: regulation of adiposity and insulin sensitivity. J. Nutr. 133: 3041-3046.
9. Brown JM, Halvorsen YD, Lea-Currie YR, Geigerman C, et al. (2001). Trans-10, cis-12, but not cis-9, trans-11, conjugated linoleic acid attenuates lipogenesis in primary cultures of stromal vascular cells from human adipose tissue. J. Nutr. 131: 2316-2321.
10. Choi Y, Kim YC, Han YB, Park Y, et al. (2000). The trans-10,cis-12 isomer of conjugated linoleic acid downregulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. J. Nutr. 130: 1920-1924.
11. Chouinard PY, Corneau L, Barbano DM, Metzger LE, et al. (1999). Conjugated linoleic acids alter milk fatty acid composition and inhibit milk fat secretion in dairy cows. J. Nutr. 129: 1579-1584.
12. Corino C, Magni S, Pastorelli G, Rossi R, et al. (2003). Effect of conjugated linoleic acid on meat quality, lipid metabolism, and sensory characteristics of dry-cured hams from heavy pigs. J. Anim. Sci. 81: 2219-2229.
13. Dugan MER, Aalhus JL, Schaefer AL and Kramer JKG (1997). The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can. J. Anim. Sci. 77: 723-725.
14. Dugan MER, Aalhus JL, Jeremiah LE, Kramer JKG, et al. (1999). The effects of feeding conjugated linoleic acid on subsequent pork quality. Can. J. Anim. Sci. 79: 45-51.
15. Etherton TD, Donkin SS and Bauman DE (1995). Mechanisms by which porcine somatotropin (pST) decreases adipose tissue growth in growing pigs. In: The Biology of Fat in Meat Animals (Smith SB and Smith DR, eds.). Am. Soc. Anim. Sci., Champaign, 53-69.
16. Evans M, Lin X, Odle J and McIntosh M (2002). Trans-10, cis-12 conjugated linoleic acid increases fatty acid oxidation in 3T3-L1 preadipocytes. J. Nutr. 132: 450-455.
17. Folch J, Lees M and Sloane Stanley GH (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226: 497-509.
18. Giesy JG, Hanson TW, Robinson M, Hafliger HC, et al. (1999). Effects of calcium salts of conjugated linoleic acid (CLA) on milk yield, fat and CLA content in milk fat in Holstein cows early in lactation. J. Dairy Sci. 82: 83.
19. Glock GE and McLean P (1953). Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem. J. 55: 400-408.
20. Gornall AG, Bardawill CJ and David MM (1949). Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 177: 751-766.
21. Hayashi AA, de Medeiros SR, Carvalho MH and Lanna DP (2007). Conjugated linoleic acid (CLA) effects on pups growth, milk composition and lipogenic enzymes in lactating rats. J. Dairy Res. 74: 160-166.
22. Ingle DL, Bauman DE, Mellenberger RW and Johnson DE (1973). Lipogenesis in the ruminant: effect of fasting and refeeding on fatty acid synthesis and enzymatic activity of sheep adipose tissue. J. Nutr. 103: 1479-1488.
23. José AAFBV, Alvares LE, Delgado EF, Coutinho LL, et al. (2006). Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures. Genet. Mol. Biol. 29: 180-186.
24. José AA, Gama MA, Urban A, Merighe GK, et al. (2007). Evaluation of polyvinyl alcohol for fatty acid supplementation in adipose tissue explant culture. Genet. Mol. Res. 6: 214-221.
25. Lanna DPD (1993). Cellular mechanisms of somatotropin control of lipogenesis and lipolysis in adipose tissue of lactating and growing animals. PhD thesis, Cornell University, Ithaca.
26. Lanna DP and Bauman DE (1999). Effect of somatotropin, insulin, and glucocorticoid on lipolysis in chronic cultures of adipose tissue from lactating cows. J. Dairy Sci. 82: 60-68.
27. Lanna DPD, Dwyer DA and Bauman DE (1994). Somatotropin and the control of lipogenesis by insulin and adenosine in pig adipose tissue. J. Anim. Sci. 72: 161.
28. Lanna DP, Houseknecht KL, Harris DM and Bauman DE (1995). Effect of somatotropin treatment on lipogenesis, lipolysis, and related cellular mechanisms in adipose tissue of lactating cows. J. Dairy Sci. 78: 1703-1712.
29. Lee KN, Pariza MW and Ntambi JM (1998). Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochem. Biophys. Res. Commun. 248: 817-821.
30. Lin X, Loor JJ and Herbein JH (2004). Trans10,cis12-18:2 is a more potent inhibitor of de novo fatty acid synthesis and desaturation than cis9,trans11-18:2 in the mammary gland of lactating mice. J. Nutr. 134: 1362-1368.
31. Littell RC, Milliken GA, Stroup WW and Wolfinger RD (1996). SAS system for mixed models. Statistical Analysis System Institute, Cary.
32. Matitashvili E, Baumgard LH and Bauman DE (2001). The effect of trans-10, cis-12 conjugated linoleic acid (CLA) infusion on milk fat synthesis and expression of lipogenic enzymes in the mammary gland of lactating cows. J. Anim. Sci. 79: 310.
33. Medeiros SR, Oliveira DE, Aroeira LJM, McGuire MA, et al. (2000). The effect of long-term supplementation of conjugated linoleic acid (CLA) to dairy cows grazing tropical pasture. J. Dairy Sci. 83: 169.
34. Mersmann HJ (2001). Mechanisms for conjugated linoleic acid-mediated reduction in fat deposition. J. Anim. Sci. 80: E126-E134.
35. Ostrowska E, Muralitharan M, Cross RF, Bauman DE, et al. (1999). Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J. Nutr. 129: 2037-2042.
36. Pariza MW, Ashoor SH, Chu FS and Lund DB (1979). Effects of temperature and time on mutagen formation in pan-fried hamburger. Cancer Lett. 7: 63-69.
37. Park Y, Albright KJ, Liu W, Storkson JM, et al. (1997). Effect of conjugated linoleic acid on body composition in mice. Lipids 32: 853-858.
38. Park Y, Storkson JM, Albright KJ, Liu W, et al. (1999). Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids 34: 235-241.
39. Park Y, Storkson JM, Ntambi JM, Cook ME, et al. (2000). Inhibition of hepatic stearoyl-CoA desaturase activity by trans-10, cis-12 conjugated linoleic acid and its derivatives. Biochim. Biophys. Acta 1486: 285-292.
40. Perfield JW, Bernal-Santos G, Overton TR and Bauman DE (2002). Effects of dietary supplementation of rumen-protected conjugated linoleic acid in dairy cows during established lactation. J. Dairy Sci. 85: 2609-2617.
41. Peterson DG, Matitashvili EA and Bauman DE (2003). Diet-induced milk fat depression in dairy cows results in increased trans-10, cis-12 CLA in milk fat and coordinate suppression of mRNA abundance for mammary enzymes involved in milk fat synthesis. J. Nutr. 133: 3098-3102.
42. Pfaffl MW (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29: e45.
43. Pfaffl MW, Horgan GW and Dempfle L (2002). Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 30: e36.
44. Piperova LS, Teter BB, Bruckental I, Sampugna J, et al. (2000). Mammary lipogenic enzyme activity, trans fatty acids and conjugated linoleic acids are altered in lactating dairy cows fed a milk fat-depressing diet. J. Nutr. 130: 2568-2574.
45. SAS Institute (1999). SAS/SAT: user's guide. SAS Intitute Inc., Cary.
46. Satory DL and Smith SB (1999). Conjugated linoleic acid inhibits proliferation but stimulates lipid filling of murine 3T3-L1 preadipocytes. J. Nutr. 129: 92-97.
47. Sinnett-Smith PA, Vernon RG and Mayer RJ (1979). Fatty acid synthesis and the activities of fatty acid synthesizing enzymes in rat adiposes tissue during pregnancy and lactation [proceedings]. Biochem. Soc. Trans. 7: 388-389.
48. Takahashi Y, Kushiro M, Shinohara K and Ide T (2002). Dietary conjugated linoleic acid reduces body fat mass and affects gene expression of proteins regulating energy metabolism in mice. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 133: 395-404.
49. Takahashi Y, Kushiro M, Shinohara K and Ide T (2003). Activity and mRNA levels of enzymes involved in hepatic fatty acid synthesis and oxidation in mice fed conjugated linoleic acid. Biochim. Biophys. Acta 1631: 265-273.
50. Thiel-Cooper RL, Parrish FC Jr, Sparks JC, Wiegand BR, et al. (2001). Conjugated linoleic acid changes swine performance and carcass composition. J. Anim. Sci. 79: 1821-1828.
51. Tricon S, Burdge GC, Kew S, Banerjee T, et al. (2004). Opposing effects of cis-9, trans-11 and trans-10,cis-12 conjugated linoleic acid on blood lipids in healthy humans. Am. J. Clin. Nutr. 80: 614-620.
52. Xu X, Storkson J, Kim S, Sugimoto K, et al. (2003). Short-term intake of conjugated linoleic acid inhibits lipoprotein lipase and glucose metabolism but does not enhance lipolysis in mouse adipose tissue. J. Nutr. 133: 663-667.