Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures

Genetics and Molecular Biology

Volumn 29, Issue 1, 2006, Pages 180-186

  José, Andrea A F B V ^a   Alvares, Lúcia E ^b   Delgado, Eduardo F ^a   Coutinho, Luiz L ^a   Lanna, Dante P D ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

Author keywords

Competitive RT PCR; Cultures of adipose tissue explants; Enzyme activity; mRNA quantification; Swine

Indexed keywords

ACTIN; COMPLEMENTARY DNA; FATTY ACID SYNTHASE; GROWTH HORMONE; MESSENGER RNA;

ACCURACY; ADIPOSE TISSUE; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DNA SEQUENCE; ENZYME ACTIVITY; EXPLANT; GENE DELETION; GENE EXPRESSION; GENETIC TRANSCRIPTION; HORMONE ACTION; IN VITRO STUDY; IN VIVO STUDY; MALE; MOLECULAR CLONING; NONHUMAN; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; STATISTICAL SIGNIFICANCE; TISSUE CULTURE; TISSUE METABOLISM;

ANIMALIA; SUIDAE; SUS SCROFA;

EID: 33645028668     PISSN: 14154757     EISSN: 16784685     Source Type: Journal    
DOI: 10.1590/S1415-47572006000100032     Document Type: Article

References (46)

1. Alvares LE, Mantoani A, Corrente JE and Coutinho LL (2003) Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos. Braz J Med Biol Res 36:1629-1641.
2. Bauman DE (1976) Intermediary metabolism of adipose tissue. Fed Proc 35:2308-2313.
3. Bauman DE (1999) Bovine somatotropin and lactation: From basic science to commercial application. Domest Anim Endocrinol 17:101-116.
4. Bauman DE and Vernon RG (1993) Effects of exogenous bovine somatotropin on lactation. Annu Rev Nutr 13:437-461.
5. Borland CA, Barber MC, Travers MT and Vernon RG (1994) Growth hormone inhibition of lipogenesis in sheep adipose tissue: Requirement for gene transcription and polyamines. J Endocrinol 142:235-243.
6. Castro FCP, Delgado EF, Bezerra RMN and Lanna DPD (2004) Effects of growth hormone on insulin signal transduction in rat adipose tissue maintained in vitro. Endocr Res 30:225-238.
7. Clarke SD (1993) Regulation of fatty acid synthase gene expression: An approach for reducing fat accumulation. J Anim Sci 71:1957-1965.
8. Dice JF and Goldberg AL (1975) Statistical analysis of the relationship between degradative rates and molecular weights of proteins. Arch Biochem Biophys 170, 213-219.
9. Donkin SS, Chiu PY, Yin D, Louveau I, Swencki B, Vockroth J, Evock-Clover CM, Peters JL and Etherton TD (1996a) Porcine somatotropin differentially down-regulates expression of GLUT4 and Fatty Acid Synthase genes in pig adipose tissue. J Nutr 126:2568-2577.
10. Donkin SS, Mcnall AD, Swencki BS, Peters JL and Etherton TD (1996b) The growth hormone - Dependent decrease in hepatic fatty acid synthase mRNA is the result of decrease in gene transcription. J Mol Endocrinol 16:151-158.
11. Dunshea FR, Harris DM, Bauman DE, Boyd RD and Bell AW (1992a) Effect of somatotropin on nonesterified fatty acid and glycerol metabolism in growing pigs. J Anim Sci 70:132-140.
12. Dunshea FR, Harris DM, Bauman DE, Boyd RD and Bell AW (1992b) Effect of porcine somatotropin on in vivo glucose kinetics and lipogenesis in growing pigs. J Anim Sci 70:141-151.
13. Etherton TD (2004) Somatotropic function: The somatomedim hypothesis revisited. J Anim Sci 82(E. Suppl.):E239-E244.
14. Etherton TD and Bauman DE (1998) Biology of Somatotropin in Growth and lactation of domestic animals. Physiol Rev 78:745-761.
15. Etherton TD, Donkin SS and Bauman DE (1995) Mechanisms by which porcine somatotropin (pST) decreases adipose tissue growth in growing pigs. In: Smith SB, Smith DR and Champaign Il. The Biology of Fat in Meat Animals. Am Soc Anim Sci, Ithaca, pp 53-69.
16. Frick F, Lindén D, Améen C, Edén S, Mode A and Oscarsson J (2002) Interaction between growth hormone and insulin in the regulation of lipoprotein metabolism in the rat. Am J Physiol Endocrinol Metab 283:E10234-E1031.
17. Foufelle F and Ferré P (2002) New perspectives in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose: A role for the transcriptional factor SREBP-1c. Biochem J 366:377-391.
18. Girard J, Chatelain F, Boillot J, Prip-Buus C, Thumelin S, Pégorier JP, Foufelle F and Férre P (1997) Nutrient regulation of gene expression. J Anim Sci 75:46-57.
19. Gornall AG, Bradawill CT and David MM (1949) Determination of serum protein by means of biuret reagent. J Biol Chem 177:751-766.
20. Harris DM, Dunshea FR, Bauman DE, Boyd RD, Wang SY, Johnson PA and Clarke SD (1993) Effect of in vivo somatotropin treatment of growing pigs on adipose tissue lipogenesis. J Anim Sci 71:3293-3300.
21. Ingle DL, Bauman DE, Mellenberger RW and Johnson DE (1973) Lipogenesis in the ruminant: Effect of fasting and refeeding on fatty acid synthesis and enzymatic activity of sheep adipose tissue. J Nutr 103:1479-1488.
22. José AAFBV, Alvares LE, Coutinho LL and Lanna DPD (1999) Padronização da técnica de RT-PCR quantitativo para avaliação da expressão gênica da FAS em suínos. Gen Mol Biol 22:639 (abstract).
23. King RH, Campbell RG, Smits RJ, Morley WC, Ronnfeldt K, Butler K and Dunshea FR (2000) Interrelationships between dietary lysine, sex, and porcine somatotropin administration on growth performance and protein deposition in pigs between 80 and 120 kg of live weight. J Anim Sci 78:2639-2651.
24. Kotake S, Jr-Schumacher HR and Wilder RL (1996) A simple nested RT-PCR method for quantification of the relative amounts of multiple cytokine mRNAs in small tissue samples. J Immunol Methods 199:193-203.
25. Lanna DPD, Dwyer DA and Bauman DE (1994) Somatotropin and the control of lipogenesis by insulin and adenosine in pig adipose tissue. J Anim Sci 72:161.
26. Lanna DPD, Houseknecht KL, Harris DM and Bauman DE (1995) Effects of somatotropin treatment on lipogenesis, lipolysis and related cellular mechanisms in adipose tissue of lactating cows. J Dairy Sci 78:1703-1712.
27. Lanna DPD and Bauman DE (1999) Effect of somatotropin, insulin, and glucocorticoid on lipolysis in chronic cultures of adipose tissue from lactating cows. J Dairy Sci 82:60-68.
28. Lee KC, Azain MJ, Hausman DB and Ramsay TG (2000) Somatotropin and adipose tissue metabolism: Substrate and temporal effects. J Anim Sci 78:1236-1246.
29. Louveau I and Gondret F (2004) growth hormone and insulin affect fatty acid synthase activity in isolated porcine adipocytes in culture without any modifications of sterol regulatory element binding protein-1 expression. J Endocrinol 181:271-280.
30. Magri KA, Adamo M, Leroith D and Etherton TD (1990) The inhibition of insulin action and glucose metabolism by porcine growth hormone in porcine adipocytes is not the result of any decrease in insulin binding or insulin receptor kinase activity. Biochem J 266:107-113.
31. McNeel RL and Mersmann HJ (1999) Distribution and quantification of beta1-, beta2-, and beta3- adrenergic receptor subtype transcripts in porcine tissues. J Anim Sci 77:611-621.
32. Mildner AM and Clarke SD (1991) Porcine fatty acid synthase: Cloning of a complementary DNA, tissue distribution of its mRNA and suppression of expression by somatotropin and dietary protein. J Nutr 121:900-907.
33. O'Hea EK and Leville GA (1969) Significance of adipose tissue and liver as sites of fatty acid synthesis in the pig and the efficiency of utilization of various substrates for lipogenesis. J Nutr 99:338-344.
34. Rehfeldt C, Nissen PM, Kuhn G, Vestergaard M, Ender K and Oksbjerg N (2004) Effects of maternal nutrition and porcine growth hormone (pGH) treatment during gestation on endocrine and metabolic factors in sows, fetuses and pigs, skeletal muscle development, and postnatal growth. Domest Anim Endocrinol 27:267-285.
35. SAS Institute, Inc. (1999) SAS/SAT User's Guide. SAS Institute Inc., Cary, NC.
36. Sillence MN (2004) Technologies for the control of fat and lean deposition in livestock. Vet J 167:242-257.
37. Sinnet-Smith PA, Vernon RG and Mayer RJ (1979) Fatty acid synthesis and the activities of fatty acid synthesizing in rat adipose tissue during pregnancy and lactation. Biochem Soc Trans 7:388-389.
38. Thirone ACP, Carvalho CR, Brenelli SL, Velloso LA and Saad MJ (1997) Effect of chronic growth hormone treatment on insulin signal transduction in rat tissues. Mol Cell Endocrinol 130:33-42.
39. Thirone ACP, Paez-Espinosa EV, Carvalho CRO and Saad MJ (1998) Regulation of insulin stimulated tyrosine phosphorylation of Shc and IRS-1 in the muscle of rats: Effect of growth hormone and epinephrine. FEBS Lett 421:191-196.
40. Towle HC, Kaytor EN and Shih H (1997) Regulation of the expression of lipogenic enzyme genes by carbohydrate. Annu Rev Nutr 17:405-433.
41. Vann RC, Nguyen HV, Reeds PJ, Burrin DG, Fiorotto ML, Steele NC, Deaver DR and Davis TA (2000) Somatotropin increases protein balance by lowering body protein degradation in fed, growing pigs. Am J Physiol Endocrinol Metab 278:E477-E483.
42. Vernon RG, Barber MC and Finley E (1991) Modulation of the activity of acetyl-CoA carboxilase and other lipogenic enzymes by growth hormone, insulin and dexamethasone in sheep adipose tissue and relationship to adaptations to lactation in sheep adipose tissue and relationship to adaptations to lactation. Biochem J 274:543-548.
43. Vernon RG and Finley E (1988) Roles of insulin and growth hormone in the adaptations of fatty acid synthesis in white adipose tissue during the lactation cycle in sheep. Biochem J 256:873-878.
44. Yin D, Clarke SD, Peters JL and Etherton TD (1998) Somatotropin-dependent decrease in fatty acid synthase mRNA abundance in 3T3-F442A adipocytes is the result of a decrease in both gene transcription and mRNA stability. Biochem J 331:815-820.
45. Yin D, Griffin MJ and Etherton TD (2001a) Analysis of the signal pathways involved in the regulation of fatty acid synthase gene expression by insulin and somatotropin. J Anim Sci 79:1194-1200.
46. Yin D, Griffin MJ and Etherton TD (2001b) Transcriptional regulation of fatty acid synthase gene by somatotropin in 3T3-F442A adipocytes. J Anim Sci 79:2336-2345.