A quick in vitro pathway from prokaryotic genomic libraries to enzyme discovery

BioTechniques

Volumn 45, Issue 1, 2008, Pages 63-68

  Ryabova, Lyubov A ^a,b   Guillemer, Sabrina ^a   Pallas, Stéphanie ^a   Persillon, Cécile ^a   Lefèvre, Fabrice ^a   Masson, Jean Michel ^a,c   Ravot, Gilles ^a  

^a Proteus SA   (France)

^b INST DE BIOL MOLEC DES PLANTES   (France)

^c INSA   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; DNA FRAGMENT; GENOMIC DNA;

ARTICLE; BACTERIAL GENOME; DNA LIBRARY; ENZYME ISOLATION; ESCHERICHIA COLI; GENE LIBRARY; NONHUMAN; OPEN READING FRAME; PROTEIN FOLDING; PSEUDOALTEROMONAS; RNA TRANSLATION; TRANSCRIPTION REGULATION; TRANSLATION INITIATION;

CELL-FREE SYSTEM; ESTERASES; GENOMIC LIBRARY; OPEN READING FRAMES; PSEUDOALTEROMONAS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; PSEUDOALTEROMONAS;

EID: 47949117756     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112820     Document Type: Article

References (20)

1. Spirin, A.S., V.I. Baranov, L.A. Ryabova, S.Y. Ovodov, and Y.B. Alakhov. 1988. Continuous cell free translation system capable of producing polypeptides in high yield. Science 242:1162-1164.
2. Kim, T.W., D.M. Kim, and C.Y. Choi. 2006. Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system. J. Biotechnol. 124:373-380.
3. Ryabova, L.A., D. Desplancq, A.S. Spirin, and A. Plückthun. 1997. Functional antibody production using cell-free translation: effects of protein disulfide isomerase and chaperones. Nat. Biotechnol. 15:79-84.
4. Kawasaki, T., M.D. Gouda, T. Sawasaki, K. Takai, and Y. Endo. 2003. Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ. Eur. J. Biochem. 270:4780-4786.
5. Jermutus, L., L.A. Ryabova, and A. Plückthun. 1998. Recent advances in producing and selecting functional proteins by using cell-free translation. Curr. Opin. Biotechnol. 9:534-548.
6. Graentzdoerffer, A. and C. Nemetz. 2003. High-throughput expression-PCR using universal plasmid-specific primers. BioTechniques 34:256-260.
7. Busso, D., R. Kim, and S.H. Kim. 2004. Using an Escherichia coli cell-free extract to screen for soluble expression of recombinant proteins. J. Struct. Funct. Genomics 5:69-74.
8. Agarwal, R.K. and A. Perl. 1993. PCR amplification of highly GC-rich DNA template after denaturation by NaOH. Nucleic Acids Res. 21:5283-5284.
9. Varadaraj, K. and D. Skinner. 1994. Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases. Gene 140:1-5.
10. Yates, J.L., A.E. Arfsten, and M. Nomura. 1980. In vitro expression of Escherichia coli ribosomal protein genes: autogenous inhibition of translation. Proc. Natl. Acad. Sci. USA 77:1837-1841.
11. Khazaie, K., J.H. Buchanan, and R.F. Rosenberger. 1984. The accuracy of Qβ RNA translation. 2. Errors during the synthesis of Qβ proteins by cell-free Escherichia coli extracts. Eur. J. Biochem. 144:491-495.
12. Zubay, G. 1973. In vitro synthesis of protein in microbial systems. Annu. Rev. Genet. 7:267-287.
13. Kim, D.M. and C.Y. Choi. 1996. A semi-continuous prokaryotic coupled transcription translation system using a dialysis membrane. Biotechnol. Prog. 12:645-649.
14. Badalassi, F., D. Wahler, G. Klein, P. Crotti, and J.L. Reymond. 2000. A versatile periodate-coupled fluorogenic assay for hydrolytic enzymes. Angew. Chem. Int. Ed. Engl. 39:4067-4070.
15. Wahler, D., F. Badalassi, P. Crotti, and J.L. Reymond. 2001. Enzyme fingerprints by fluorogenic and chromogenic substrate arrays. Angew. Chem. Int. Ed. Engl. 40:4457-4460.
16. Kim, H.E., I.S. Lee, J.H. Kim, K.W. Hahn, U.J. Park, H.S. Han, and K.R. Park. 2003. Gene cloning, sequencing, and expression of an esterase from Acinetobacter lwoffii I6C-1. Curr. Microbiol. 46:291-295.
17. Adhin, M.R. and J. van Duin. 1990. Scanning model for translational reinitiation in eubacteria. J. Mol. Biol. 213:811-818.
18. de Smit, M. and J. van Duin. 1990. Control of prokaryotic translational initiation by mRNA secondary structure. Prog. Nucleic Acid Res. Mol. Biol. 38:1-35.
19. Sergeeva, A., M.G. Kolonin, J.J. Molldrem, R. Pasqualini, and W. Arap. 2006. Display technologies: application for the discovery of drug and gene delivery agents. Adv. Drug Deliv. Rev. 58:1622-1654.
20. Amstutz, P., P. Forrer, C. Zahnd, and A. Plückthun. 2001. In vitro display technologies: novel developments and applications. Curr. Opin. Biotechnol. 12:400-405.