Genotypic diversity, antimicrobial resistance, and virulence factors of human isolates and probiotic cultures constituting two intraspecific groups of Enterococcus faecium isolates

Applied and Environmental Microbiology

Volumn 74, Issue 14, 2008, Pages 4247-4255

  Vankerckhoven, Vanessa ^a   Huys, Geert ^b   Vancanneyt, Marc ^c   Snauwaert, Cindy ^b   Swings, Jean ^b,c   Klare, Ingo ^d   Witte, Wolfgang ^d   Van Autgaerden, Tim ^a   Chapelle, Sabine ^a   Lammens, Christine ^a   Goossens, Herman ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

^c GHENT UNIVERSITY   (Belgium)

^d ROBERT KOCH INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COLLOIDS; COSMIC RAYS; ELECTROPHORESIS; GELATION; GENES; NUCLEIC ACIDS; ORGANIC ACIDS; SAFETY TESTING;

AMERICAN SOCIETY; AMPLIFIED FRAGMENT LENGTH POLYMORPHISM (AFLP); ANTIMICROBIAL AGENTS; ANTIMICROBIAL RESISTANCE (AMR); CLINICAL ISOLATES; ENTEROCOCCUS FAECIUM; FINGERPRINTING TECHNIQUES; GENOMIC GROUPS; GENOTYPIC DIVERSITY; HIGH RESOLUTIONS; HIGH VARIABILITY; MICRODILUTION; MULTIPLEX PCR; PROBIOTIC; PROBIOTIC CULTURES; PULSED-FIELD GEL ELECTROPHORESIS (PFGE); RESISTANCE GENES; VIRULENCE FACTORS; VIRULENCE GENES;

HEALTH;

AMPICILLIN; CHLORAMPHENICOL; DALFOPRISTIN PLUS QUINUPRISTIN; ERYTHROMYCIN; FUSIDIC ACID; GENTAMICIN; LINEZOLID; PENICILLIN G; PROBIOTIC AGENT; STREPTOMYCIN; SULTAMICILLIN; TEICOPLANIN; TETRACYCLINE; VANCOMYCIN; VIRULENCE FACTOR;

ANTIBIOTIC RESISTANCE; ANTIMICROBIAL ACTIVITY; BACTERIUM; COMPARATIVE STUDY; DNA FINGERPRINTING; GENOTYPE; PROBIOTICS; SPECIES DIVERSITY; VIRULENCE;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM CULTURE; BACTERIUM ISOLATE; BROTH DILUTION; CONTROLLED STUDY; DNA FINGERPRINTING; ENTEROCOCCUS FAECIUM; GENETIC VARIABILITY; GENOTYPE; INTRASPECIFIC VARIATION; NONHUMAN; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS; ANTI-BACTERIAL AGENTS; BACTERIAL TYPING TECHNIQUES; CLUSTER ANALYSIS; DRUG RESISTANCE, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECIUM; FECES; FOOD MICROBIOLOGY; GENE FREQUENCY; GENOTYPE; HUMANS; MICROBIAL SENSITIVITY TESTS; PHENOTYPE; POLYMERASE CHAIN REACTION; PROBIOTICS; VARIATION (GENETICS); VIRULENCE FACTORS;

ENTEROCOCCUS FAECIUM;

EID: 47849101550     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02474-07     Document Type: Article

References (59)

1. Barbier, N., P. Saulnier, E. Chachaty, S. Dumontier, and A. Andremont. 1996. Random amplified polymorphic DNA typing versus pulsed-field gel electrophoresis for epidemiological typing of vancomycin-resistant enterococci. J. Clin. Microbiol. 34:1096-1099.
2. Barbosa, T. M., K. P. Scott, and H. J. Flint. 1999. Evidence for recent intergeneric transfer of a new tetracycline resistance gene, tet(W), isolated from Butyrivibrio fibrisolvens, and the occurrence of tet(O) in ruminal bacteria. Environ. Microbiol. 1:53-64.
3. Brisson-Noel, A., S. Dutka-Malen, C. Molinas, R. Leclercq, and P. Courvalin. 1990. Cloning and heterospecific expression of the resistance determinant vanA encoding high-level resistance to glycopeptides in Enterococcus faecium BM4147. Antimicrob. Agents Chemother. 34:924-927.
4. Clermont, D., O. Chesneau, C. G. De, and T. Horaud. 1997. New tetracycline resistance determinants coding for ribosomal protection in streptococci and nucleotide sequence of tet(T) isolated from Streptococcus pyogenes A498. Antimicrob. Agents Chemother. 41:112-116.
5. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing; 15th informational supplement, M100-S15, vol. 25, no. 1. Clinical and Laboratory Standards institute, Wayne, PA.
6. Collignon, P., and J. Turnidge. 1999. Fusidic acid in vitro activity. Int. J. Antimicrob. Agents 12(Suppl. 2):S45-S58.
7. Descheemaeker, P., C. Lammens, B. Pot, P. Vandamme, and H. Goossens. 1997. Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human medicine. Int. J. Syst. Bacteriol. 47:555-561.
8. Eaton, T. J., and M. J. Gasson. 2001. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl. Environ. Microbiol. 67:1628-1635.
9. European Food Safety Authority. 2005. Opinion of the Scientific Panel on Additives and Products or Substance Used in Animal Feed on the updating of the criteria used in the assessment of bacteria for resistance to antibiotics of human or veterinary importance. EFSA J. 223:1-12.
10. Fluit, A. C., M. R. Visser, and F. J. Schmitz. 2001. Molecular detection of antimicrobial resistance. Clin. Microbiol. Rev. 14:836-871.
11. Franz, C. M., W. H. Holzapfel, and M. E. Stiles. 1999. Enterococci at the crossroads of food safety? Int. J. Food Microbiol. 47:1-24.
12. Franz, C. M., A. B. Muscholl-Silberhorn, N. M. Yousif, M. Vancanneyt, J. Swings, and W. H. Holzapfel. 2001. Incidence of virulence factors and antibiotic resistance among enterococci isolated from food. Appl. Environ. Microbiol. 67:4385-4389.
13. Franz, C. M., M. E. Stiles, K. H. Schleifer, and W. H. Holzapfel. 2003. Enterococci in foods - a conundrum for food safety. Int. J. Food Microbiol. 88:105-122.
14. Galli, D., F. Lottspeich, and R. Wirth. 1990. Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1. Mol. Microbiol. 4:895-904.
15. Gevers, D., G. Huys, and J. Swings. 2001. Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol. Lett. 205:31-36.
16. Giraffa, G., D. Carminati, and E. Neviani. 1997. Enterococci isolated from dairy products: a review of risks and potential technological use. J. Food Prot. 60:732-738.
17. Homan, W. L., D. Tribe, S. Poznanski, M. Li, G. Hogg, E. Spalburg, J. D. van Embden, and R. J. Willems. 2002. Multilocus sequence typing scheme for Enterococcus faecium. J. Clin. Microbiol. 40:1963-1971.
18. Ike, Y., H. Hashimoto, and D. B. Clewell. 1987. High incidence of hemolysin production by Enterococcus (Streptococcus) faecalis strains associated with human parenteral infections. J. Clin. Microbiol. 25:1524-1528.
19. Janssen, P., R. Coopman, G. Huys, J. Swings, M. Bleeker, P. Vos, M. Zabeau, and K. Kersters. 1996. Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. Microbiology 142:1881-1893.
20. Johnson, A. P. 1994. The pathogenicity of enterococci. J. Antimicrob. Chemother. 33:1083-1089.
21. Klare, I., C. Konstabel, S. Müller-Bertling, R. Reissbrodt, G. Huys, M. Vancanneyt, J. Swings, H. Goossens, and W. Witte. 2005. Evaluation of new broth media for microdilution antibiotic susceptibility testing of lactobacilli, pediococci, lactococci, and bifidobacteria. Appl. Environ. Microbiol. 71:8982-8986.
22. Klare, I., C. Konstabel, G. Werner, G. Huys, V. Vankerckhoven, G. Kahlmeter, B. Hildebrandt, S. Müller-Bertling, W. Witte, and H. Goossens. 2007. Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use. J. Antimicrob. Chemother. 59:900-912.
23. Kuhn, I., L. G. Burman, S. Haeggman, K. Tullus, and B. E. Murray. 1995. Biochemical fingerprinting compared with ribotyping and pulsed-field gel electrophoresis of DNA for epidemiological typing of enterococci. J. Clin. Microbiol. 33:2812-2817.
24. Lyras, D., and J. I. Rood. 1996. Genetic organization and distribution of tetracycline resistance determinants in Clostridium perfringens. Antimicrob. Agents Chemother. 40:2500-2504.
25. Mannu, L., A. Paba, E. Daga, R. Comunian, S. Zanetti, I. Dupre, and L. A. Sechi. 2003. Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int. J. Food Microbiol. 88:291-304.
26. Moellering, R. C., Jr. 1992. Emergence of Enterococcus as a significant pathogen. Clin. Infect. Dis. 14:1173-1176.
27. Murray, B. E. 1990. The life and times of the Enterococcus. Clin. Microbiol. Rev. 3:46-65.
28. Nikolich, M. P., N. B. Shoemaker, and A. A. Salyers. 1992. A Bacteroides tetracycline resistance gene represents a new class of ribosome protection tetracycline resistance. Antimicrob. Agents Chemother. 36:1005-1012.
29. Patel, R. 2003. Clinical impact of vancomycin-resistant enterococci. J. Antimicrob. Chemother. 51(Suppl. 3):iii13-iii21.
30. Patel, R., J. R. Uhl, P. Kohner, M. K. Hopkins, J. M. Steckelberg, B. Kline, and F. R. Cockerill III. 1998. DNA sequence variation within vanA, vanB, vanC-1, and vanC-2/3 genes of clinical Enterococcus isolates. Antimicrob. Agents Chemother. 42:202-205.
31. Perreten, V., F. Schwarz, L. Cresta, M. Boeglin, G. Dasen, and M. Teuber. 1997. Antibiotic resistance spread in food. Nature 389:801-802.
32. Pitcher, D. G., N. A. Saunders, and R. J. Owen. 1989. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Bacteriol. 8:151-156.
33. Portillo, A., F. Ruiz-Larrea, M. Zarazaga, A. Alonso, J. L. Martinez, and C. Torres. 2000. Macrolide resistance genes in Enterococcus spp. Antimicrob. Agents Chemother. 44:967-971.
34. Pot, B., P. Vandamme, and K. Kersters. 1994. Analysis of electrophoretic whole-organism protein fingerprints, p. 493-521. In M. Goodfellow and A. G. O'Donnell (ed.), Modern microbial methods. Chemical methods in prokaryotic systematics. J. Wiley and Sons Ltd., Chichester, United Kingdom.
35. Quednau, M., S. Ahrné, and G. Molin. 1999. Genomic relationships between Enterococcus faecium strains from different sources and with different antibiotic resistance profiles evaluated by restriction endonuclease analysis of total chromosomal DNA using EcoRI and PvuII. Appl. Environ. Microbiol. 65:1777-1780.
36. Quintiliani, R., Jr., S. Evers, and P. Courvalin. 1993. The vanB gene confers various levels of self-transferable resistance to vancomycin in enterococci. J. Infect. Dis. 167:1220-1223.
37. Efm, predominates in Enterococcus faecium of clinical origin. J. Infect. Dis. 187:508-512.
38. Semedo, T., M. A. Santos, M. F. Lopes, J. J. Figueiredo Marques, M. T. Barreto Crespo, and R. Tenreiro. 2003. Virulence factors in food, clinical and reference enterococci: a common trait in the genus? Syst. Appl. Microbiol. 26:13-22.
39. Shankar, V., A. S. Baghdayan, M. M. Huycke, G. Lindahl, and M. S. Gilmore. 1999. Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein. Infect. Immun. 67:193-200.
40. Strommenger, B., C. Kettlitz, G. Werner, and W. Witte. 2003. Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J. Clin. Microbiol. 41:4089-4094.
41. Su, Y. A., M. C. Sulavik, P. He, K. K. Makinen, P. L. Makinen, S. Fiedler, R. Wirth, and D. B. Clewell. 1991. Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens. Infect. Immun. 59:415-420.
42. Temmerman, R., B. Pot, G. Huys, and J. Swings. 2003. Identification and antibiotic susceptibility of bacterial isolates from probiotic products. Int. J. Food Microbiol. 81:1-10.
43. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.
44. Toma, E., and D. Barriault. 1995. Antimicrobial activity of fusidic acid and disk diffusion susceptibility testing criteria for gram-positive cocci. J. Clin. Microbiol. 33:1712-1715.
45. Tomayko, J. F., and B. E. Murray. 1995. Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis. J. Clin. Microbiol. 33:2903-2907.
46. Top, J., L. M. Schouls, M. J. Bonten, and R. J. Willems. 2004. Multiple-locus variable-number tandem repeat analysis, a novel typing scheme to study the genetic relatedness and epidemiology of Enterococcus faecium isolates. J. Clin. Microbiol. 42:4503-4511.
47. Vancanneyt, M., G. Huys, K. Lefebvre, V. Vankerckhoven, H. Goossens, and J. Swings. 2006. Intraspecific genotypic characterization of Lactobacillus rhamnosus strains intended for probiotic use and isolates of human origin. Appl. Environ. Microbiol. 72:5376-5383.
48. Vancanneyt, M., A. Lombardi, C. Andrighetto, E. Knijff, S. Torriani, K. J. Bjorkroth, C. M. Franz, M. R. Foulquie Moreno, H. Revets, L. De Vuyst, J. Swings, K. Kersters, F. Dellaglio, and W. H. Holzapfel. 2002. Intraspecies genomic groups in Enterococcus faecium and their correlation with origin and pathogenicity. Appl. Environ. Microbiol. 68:1381-1391.
49. Vankerckhoven, V., T. Van Autgaerden, G. Huys, M. Vancanneyt, J. Swings, and H. Goossens. 2004. Establishment of the PROSAFE collection of probiotic and human lactic acid bacteria. Microb. Ecol. Health Dis. 16:131-136.
50. Vankerckhoven, V., T. Van Autgaerden, C. Vael, C. Lammens, S. Chapelle, R. Rossi, D. Jabes, and H. Goossens. 2004. Development of a multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium. J. Clin. Microbiol. 42:4473-4479.
51. Werner, G., B. Hildebrandt, I. Klare, and W. Witte. 2000. Linkage of determinants for streptogramin A, macrolide-lincosamide-streptogramin B, and chloramphenicol resistance on a conjugative plasmid in Enterococcus faecium and dissemination of this cluster among streptogramin-resistant enterococci. Int. J. Med. Microbiol. 290:543-548.
52. Werner, G., B. Hildebrandt, and W. Witte. 2001. Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium. Antimicrob. Agents Chemother. 45:3267-3269.
53. Werner, G., I. Klare, and W. Witte. 1998. Association between quinupristin/dalfopristin resistance in glycopeptide-resistant Enterococcus faecium and the use of additives in animal feed. Eur. J. Clin. Microbiol. Infect. Dis. 17:401-402.
54. Werner, G., I. Klare, and W. Witte. 2002. Molecular analysis of streptogramin resistance in enterococci. Int. J. Med. Microbiol. 292:81-94.
55. Werner, G., R. J. Willems, B. Hildebrandt, I. Klare, and W. Witte. 2003. Influence of transferable genetic determinants on the outcome of typing methods commonly used for Enterococcus faecium. J. Clin. Microbiol. 41:1499-1506.
56. Werner, G., and W. Witte. 1999. Characterization of a new enterococcal gene, satG, encoding a putative acetyltransferase conferring resistance to streptogramin A compounds. Antimicrob. Agents Chemother. 43:1813-1814.
57. Willems, R. J., J. Top, N. van Den Braak, A. van Belkum, H. Endtz, D. Mevius, E. Stobberingh, A. van Den Bogaard, and J. D. van Embden. 2000. Host specificity of vancomycin-resistant Enterococcus faecium. J. Infect. Dis. 182:816-823.
58. World Health Organization and Food and Agricultural Organization of the United Nations. 2001. Report of a joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. London, Ontario, Canada.
59. World Health Organization and Food and Agricultural Organization of the United Nations. 2002. Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada.