Arginine coordination in enzymatic phosphoryl transfer: Evaluation of the effect of Arg166 mutations in Escherichia coli alkaline phosphatase

Biochemistry

Volumn 47, Issue 29, 2008, Pages 7663-7672

  O'Brien, Patrick J ^a,b   Lassila, Jonathan Kyle ^a   Fenn, Timothy D ^a   Zalatan, Jesse G ^a   Herschlag, Daniel ^a  

^a STANFORD UNIVERSITY   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CATALYSIS; COORDINATION REACTIONS; ENZYMES; ESCHERICHIA COLI; FOOD ADDITIVES; HYDROLYSIS; STABILIZATION;

ACTIVE SITES; ALKALINE PHOSPHATASE (ALPASE); AMERICAN CHEMICAL SOCIETY (ACS); ARGININE (ARG); ARGININE RESIDUES; BINDING INTERACTIONS; CHEMICAL TRANSITION; COVALENT ENZYMES; ENERGY PROFILING; ENZYME STRUCTURES; INDIVIDUAL (PSS 544-7); PHOSPHORYL TRANSFER; PHOSPHORYL TRANSFER REACTIONS; POSITIVE CHARGES; REACTION STEPS; SIDE CHAINS; SITE DIRECTED MUTAGENESIS; SUBSTITUTION EFFECTS; TRANSITION STATE (TS); TRANSITION STATE STABILIZATION; WILD-TYPE (WT) ENZYME; X-RAY DIFFRACTION STRUCTURE;

AMINO ACIDS;

ALKALINE PHOSPHATASE; ARGININE; GUANIDINE; PHOSPHATE;

ARTICLE; CATALYSIS; ENZYME PHOSPHORYLATION; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE MUTATION; HYDROLYSIS; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS; WILD TYPE; X RAY DIFFRACTION;

ALKALINE PHOSPHATASE; ARGININE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOSPHATES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; X-RAY DIFFRACTION;

ESCHERICHIA COLI;

EID: 47649087237     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi800545n     Document Type: Article

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