Purification and subcellular localization of a secreted 75 kDa Trypanosoma cruzi serine oligopeptidase

Acta Tropica

Volumn 107, Issue 2, 2008, Pages 159-167

  Silva Lopez, Raquel Elisa da ^a   Morgado Díaz, José Andrés ^b   Santos, Priscila Tavares dos ^a   Giovanni De Simone, Salvatore ^a,c  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b INSTITUTO NACIONAL DE CÂNCER   (Brazil)

^c FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

Author keywords

Extracellular serine peptidase; Purification; Reservosomes; Trypanosoma cruzi

Indexed keywords

1,10 PHENANTHROLINE; AGAROSE; ALPHA N 4 TOSYLARGININE METHYL ESTER; ANTISERUM; APROTININ; ARGININE; ARGININE DERIVATIVE; CALCIUM ION; DIMETHYL SULFOXIDE(N ALPHA TOSYLLYSYLCHLOROMETHYLKETONE); KETONE DERIVATIVE; NITROGEN DERIVATIVE; PEPTIDASE; POLYPEPTIDE; PROTEINASE INHIBITOR; SERINE OLIGOPEPTIDASE; TOSYLPHENYLALANYL CHLOROMETHYL KETONE; ZINC ION;

ELECTROKINESIS; ENZYME ACTIVITY; INHIBITION; PRECIPITATION (CHEMISTRY); PROTOZOAN; SECRETION;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL MEMBRANE; CELLULAR DISTRIBUTION; CHEMICAL BOND; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM VESICLE; ELUTION; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME STRUCTURE; EPIMASTIGOTE; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PROTEIN DEGRADATION; SUPERNATANT; TEMPERATURE DEPENDENCE; TRYPANOSOMA CRUZI;

ANIMALS; CULTURE MEDIA; CYTOPLASMIC VESICLES; HYDROGEN-ION CONCENTRATION; KINETICS; MICROSCOPY, FLUORESCENCE; PEPTIDE HYDROLASES; PROTEASE INHIBITORS; PROTOZOAN PROTEINS; RABBITS; SERINE; SERINE ENDOPEPTIDASES; SUBCELLULAR FRACTIONS; SUBSTRATE SPECIFICITY; TEMPERATURE; TRYPANOSOMA CRUZI;

ORYCTOLAGUS CUNICULUS; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 47549105099     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actatropica.2008.05.016     Document Type: Article

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