Mycobacterium tuberculosis isocitrate lyase [MtbIcl]: Role of divalent cations in modulation of functional and structural properties

Proteins: Structure, Function and Genetics

Volumn 72, Issue 3, 2008, Pages 892-900

  Kumar, Ranjeet ^a   Bhakuni, Vinod ^a  

^a CENTRAL DRUG RESEARCH INSTITUTE   (India)

Author keywords

Divalent cations; Enzymatic activity; Secondary structure; Stability

Indexed keywords

CADMIUM; DIVALENT CATION; ISOCITRATE LYASE; MAGNESIUM; MANGANESE; SOLVENT; ZINC;

ARTICLE; CATALYSIS; DENATURATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME REGULATION; ENZYME STABILITY; ENZYME STRUCTURE; MOLECULAR DYNAMICS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN INTERACTION; ULTRAVIOLET RADIATION;

CATALYTIC DOMAIN; CATIONS, DIVALENT; CIRCULAR DICHROISM; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; ISOCITRATE LYASE; MODELS, MOLECULAR; MYCOBACTERIUM TUBERCULOSIS; PROTEIN FOLDING; SALTS; STATIC ELECTRICITY; TEMPERATURE;

MYCOBACTERIUM TUBERCULOSIS;

EID: 47349113938     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.21984     Document Type: Article

References (18)

1. Kochi A. The global tuberculosis situation and the new control strategy of the World Health Organization. 1991. Bull World Health Organ 2001;79:71-75.
2. Smith CV, Sharma V, Sacchettini JC. TB drug discovery: addressing issues of persistence and resistance. Tuberculosis 2004;84:45-55.
3. Hoener Zu, Bentrup K, Miczak A, Swanson DL, Russell DG. Characterization of activity and expression of isocitrate lyase in Mycobacterium avium and Mycobacterium tuberculosis. J Bacteriol 1999;181:7161-7167.
4. Graham JE, Clark-curtiss JE. Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS). Proc Natl Acad Sci USA 1996;20:11554-11559.
5. Sharma V, Sharma S, Hoener Zu Bentrup K, McKinney JD, Russell DG, Jacobs WR Jr, Sacchettini JC. Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis. Nat Struct Biol 2000;7:663-668.
6. Hoyt JC, Robertson EF, Berlyn KA, Reeves HC. Escherichia coli isocitrate lyase: properties and comparisons. Biochim Biophys Acta 1988;966:30-35.
7. Serrano JA, Camacho M, Bonete MJ. Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii. FEBS Lett 1998;434:13-16.
8. Lopez-Boado YS, Herrero P, Fernandez MT, Fernandez R, Moreno R. Purification of isocitrate lyase from Saccharomyces cerevisiae. Yeast 1988;4:41-46.
9. Lorenz MC, Fink GR. Life and death in a macrophage: role of the glyoxylate cycle in virulence. Eukaryot Cell 2002;5:57-62.
10. 2+ in the structure and activity of maize (Zea mays L.) isocitrate lyase: indications for hysteretic behavior. Biochem J 1997;327:171-176.
11. Nakazawa M, Minami T, Teramura K, Kumamoto S, Hanato S, Takenaka S, Ueda M, Inui H, Nakano Y, Miyakake K. Molecular characterization of a bifunctional glyoxylate cycle enzyme, maiate synthase/isocitrate lyase, in Euglena gracilis. Comp Biochem Physiol B Biochem Mol Biol 2005;141:445-452.
12. 2+ on isocitrate lyase, a non-essentially metal-ion-activated enzyme. A graphical approach for the discrimination of the model for activation. Biochem J 1991;276:223-230.
13. +-binding domain. Protein Sci 2005;14:980-992.
14. Singh K, Bhakuni V. Cation induced differential effect on structural and functional properties of Mycobacterium tuberculosis alpha-isopropylmalate synthase. BMC Struct Biol 2007;19:7-39.
15. Akhtar MS, Bhakuni V. Streptococcus pneumoniae hyaluronate lyase contains two non-cooperative independent folding/unfolding structural domains: characterization of functional domain and inhibitors of enzyme. J Biol Chem 2003;278:25509-255016.
16. Campanero-Rhodes M, Menendez M, Saiz JL, Sanz L, Calvet JJ, Solis D. Zinc ions induce the unfolding and self-association of boar spermadhesin PSP-I, a protein with a single CUB domain architecture, and promote its binding to heparin. Biochemistry 2006;45:8227-8235.
17. Dixon GH, Kornberg HL. Assay methods for the key enzyme of the Glyoxalate Cycle. Biochem J 1959;72:3.
18. Bradford MM. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976;72:248-254.