Molecular characterization of a bifunctional glyoxylate cycle enzyme, malate synthase/isocitrate lyase, in Euglena gracilis

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 141, Issue 4, 2005, Pages 445-452

  Nakazawa, Masami ^a   Minami, Tomomi ^a   Teramura, Koji ^a   Kumamoto, Shohei ^a   Hanato, Sayaka ^a   Takenaka, Shigeo ^a   Ueda, Mitsuhiro ^a   Inui, Hiroshi ^a   Nakano, Yoshihisa ^a   Miyatake, Kazutaka ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Ace operon; Acetyl CoA; Bifunctional enzyme; Euglena gracilis; Gene fusion; Glyoxylate cycle; Inverted order; Isocitrate lyase; Malate synthase; Mitochondrial localization

Indexed keywords

ACETYL COENZYME A; ALCOHOL; ISOCITRATE LYASE; MALATE SYNTHASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CAENORHABDITIS ELEGANS; CARBON SOURCE; CATALYSIS; CONTROLLED STUDY; EUGLENA GRACILIS; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN;

CAENORHABDITIS ELEGANS; EUGLENA GRACILIS;

EID: 22144476123     PISSN: 10964959     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpc.2005.05.006     Document Type: Article

References (32)

1. D.M. Anstrom, K. Kallio, and S.J. Remington Structure of the Escherichia coli malate synthase G: pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 Å resolution Protein Sci. 12 2003 1822 1832
2. T. Blumenthal Trans-splicing and polycistronic transcription in Caenorhabditis elegans Trends Genet. 11 1995 132 136
3. T. Blumenthal, D. Evans, C.D. Link, A. Guffanti, D. Lawson, J. Thierry Mieg, D. Thierry Mieg, W.L. Chiu, K. Duke, M. Kiraly, and S.K. Kim A global analysis of Caenorhabditis elegans operons Nature 417 2002 851 854
4. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
5. K. Britton, S. Langridge, P.J. Baker, K. Weeradechapon, S.E. Sedelnikova, J.R. De Lucas, D.W. Rice, and G. Turner The crystal structure and active site location of isocitrate lyase from the fungus Aspergillus nidulans Struct. Fold Des. 8 2000 349 362
6. N. Collins, and M.J. Merrett Microbody-marker enzymes during transition from phototrophic to organotrophic growth in Euglena Plant Physiol. 55 1975 1018 1022
7. J.R. Cook Properties of partially purified malate synthase for Euglena gracilis J. Protozool. 17 1970 232 235
8. M. Cramer, and J. Myers Growth and photosynthetic characteristics of Euglena gracilis Arch. Mikrobiol. 17 1952 384 402
9. P.J. Eastmond, and I.A. Graham Re-examining the role of the glyoxylate cycle in oilseeds Trends Plant Sci. 6 2001 72 78
10. E. Giachetti, G. Pinzauti, R. Bonaccorsi, and P. Vanni Isocitrate lyase from Pinus pinea. Characterization of its true substrate and the action of magnesium ions Eur. J. Biochem. 172 1988 85 91
11. L.B.J. Graves, R.N. Trelease, A. Grill, and W.M. Becker Localization of glyoxylate cycle enzymes in glyoxysomes in Euglena J. Protozool. 19 1972 527 532
12. A. Hartig, M.M. Simon, T. Schuster, J.R. Daugherty, H.S. Yoo, and T.G. Cooper Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae Nucleic Acids Res. 20 1992 5677 5686
13. B.R. Howard, J.A. Endrizzi, and S.J. Remington Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2.0 Å resolution: mechanistic implications Biochemistry 39 2000 3156 3168
14. H. Inui, K. Miyatake, Y. Nakano, and S. Kitaoka Synthesis of reserved polysaccharide from wax esters accumulated as the result of anaerobic energy generation in Euglena gracilis returned from anaerobic to aerobic conditions Int. J. Biochem. 24 1992 799 803
15. H.L. Kornberg, and H.A. Krebs Synthesis of cell constituents from C2-units by a modified tricarboxylic acid cycle Nature 179 1957 988 991
16. H.L. Kornberg The role and control of the glyoxylate cycle in Escherichia coli Biochem. J. 99 1966 1 11
17. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
18. F. Liu, J.D. Thatcher, J.M. Barral, and H.F. Epstein Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle Dev. Biol. 169 1995 399 414
19. F. Liu, J.D. Thatcher, and H.F. Epstein Induction of glyoxylate cycle expression in Caenorhabditis elegans: a fasting response throughout larval development Biochemistry 36 1997 255 260
20. M.C. Lorenz, and G.R. Fink The glyoxylate cycle is required for fungal virulence Nature 412 2001 83 86
21. O.P. Malhotra, and P.K. Srivastava Isolation and characterization of isocitrate lyase of castor endosperm Arch. Biochem. Biophys. 214 1982 164 171
22. I. Molina, M.T. Pellicer, J. Badia, J. Aguilar, and L. Baldoma Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme Eur. J. Biochem. 224 1994 541 548
23. Y. Oda, Y. Nakano, and S. Kitaoka Utilization and toxicity of exogenous amino acid in Euglena gracilis J. Gen. Microbiol. 128 1982 853 858
24. K. Ono, M. Kondo, T. Osafune, K. Miyatake, H. Inui, S. Kitaoka, M. Nishimura, and Y. Nakano Presence of glyoxylate cycle enzymes in the mitochondria of Euglena gracilis J. Eukaryot. Microbiol. 50 2003 92 96
25. J.A. Serrano, M. Camacho, and M.J. Bonete Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii FEBS Lett. 434 1998 13 16
26. V. Sharma, S. Sharma, K. Hoener zu Bentrup, J.D. McKinney, D.G. Russell, W.R.J. Jacobs, and J.C. Sacchettini Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis Nat. Struct. Biol. 7 2000 663 668
27. M. Shirzadegan, P. Christie, and J.R. Seemann An efficient method for isolation of RNA from tissue cultured plant cells Nucleic Acids Res. 19 1991 6055
28. A. Stechmann, and T. Cavalier-Smith Rooting the eukaryote tree by using a derived gene fusion Science 297 2002 89 91
29. R.L. Tatusov, E.V. Koonin, and D.J. Lipman A genomic perspective on protein families Science 278 1997 631 637
30. K.M. Taylor, C.P. Kaplan, X. Gao, and A. Baker Localization and targeting of isocitrate lyases in Saccharomyces cerevisiae Biochem. J. 319 1996 255 262
31. L.H. Tessier, M. Keller, R.L. Chan, R. Fournier, J.H. Weil, and P. Imbault Short leader sequences may be transferred from small RNAs to pre-mature mRNAs by trans-splicing in Euglena EMBO J. 10 1991 2621 2625
32. E. Woodcock, and M.J. Merrett Purification and immunochemical characterization of malate synthase from Euglena gracilis Biochem. J. 173 1978 95 101