Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid

Applied Microbiology and Biotechnology

Volumn 80, Issue 1, 2008, Pages 87-97

  Rustler, Sven ^a   Motejadded, Hassan ^a   Altenbuchner, Josef ^a   Stolz, Andreas ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

(S) mandelic acid; Acidotolerance; Biotransformation; Manihot esculenta; Nitrilase; Oxynitrilase; Pichia pastoris; Pseudomonas fluorescens

Indexed keywords

ALDEHYDES; CATALYST ACTIVITY; CYANIDES; ESCHERICHIA COLI; GENE ENCODING; PH; PH EFFECTS;

(S)-MANDELIC ACID; ACIDIC CONDITIONS; ACIDIC PH VALUES; ACIDOTOLERANCE; BIOTRANSFORMATION; CHIRAL ANALYSIS; ENANTIOMERIC EXCESS; EXPRESSION SYSTEM; MANDELIC ACID; MANIHOT ESCULENTA; METHYLOTROPHIC YEASTS; NEUTRAL CONDITIONS; NITRILASE; OXYNITRILASE; P H VALUES; P. PASTORIS; PICHIA PASTORIS; PSEUDOMONAS FLUORESCENS; RECOMBINANT STRAINS; RESTING CELLS; WHOLE CELLS;

CATALYSIS;

BENZALDEHYDE; CYANIDE; MANDELIC ACID; NITRILASE;

BACTERIUM; BIOTRANSFORMATION; CATALYSIS; CYANIDE; DICOTYLEDON; ENZYME ACTIVITY; GENE EXPRESSION; HYDROLYSIS; ORGANIC ACID; PH; RECOMBINATION; TOLERANCE; YEAST;

ACID TOLERANCE; ACIDITY; ARTICLE; BIOTRANSFORMATION; CASSAVA; CATALYST; CHIRALITY; ENZYME ACTIVITY; ESCHERICHIA COLI; FUNGAL STRAIN; METHYLOTROPHIC YEAST; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PSEUDOMONAS FLUORESCENS; SYNTHESIS;

ALDEHYDE-LYASES; AMINOHYDROLASES; BACTERIAL PROTEINS; BENZALDEHYDES; CYANATES; GENE EXPRESSION; GENETIC ENGINEERING; HYDROGEN-ION CONCENTRATION; MANDELIC ACIDS; MANIHOT; PICHIA; PLANT PROTEINS; PSEUDOMONAS FLUORESCENS;

ESCHERICHIA COLI; MANIHOT ESCULENTA; PICHIA PASTORIS; PSEUDOMONAS FLUORESCENS;

EID: 47149110651     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1531-1     Document Type: Article

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