Use of primers based on the heat shock protein genes hsp70, hsp40, and hsp10, for the detection of bovine mastitis pathogens Streptococcus agalactiae, Streptococcus uberis and Streptococcus bovis

Molecular and Cellular Probes

Volumn 22, Issue 4, 2008, Pages 262-266

  Chiang, Yu Cheng ^a   Pai, Wan Yu ^a   Chen, Chih Yuan ^a   Tsen, Hau Yang ^a  

^a HUNGKUANG UNIVERSITY   (Taiwan)

Author keywords

Heat shock protein; Mastitis; PCR; Streptococcus agalactiae; Streptococcus bovis; Streptococcus uberis

Indexed keywords

HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 10; HEAT SHOCK PROTEIN 40; HEAT SHOCK PROTEIN 70;

ARTICLE; BACTERIUM DETECTION; COW; FALSE POSITIVE RESULT; MASTITIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS BOVIS; STREPTOCOCCUS UBERIS;

ANIMALS; CATTLE; CHAPERONIN 10; DNA PRIMERS; HEAT-SHOCK PROTEINS; HSP40 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; MASTITIS, BOVINE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; STREPTOCOCCUS;

BACTERIA (MICROORGANISMS); BOVINAE; STREPTOCOCCUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS BOVIS; STREPTOCOCCUS UBERIS;

EID: 46849108951     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2008.05.004     Document Type: Article

References (31)

1. Amann R.I., Ludwig W., and Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59 1 (1995) 143-169
2. Acinas S.G., Marcelino L.A., Klepac-Ceraj V., and Polz M.F. Divergence and Redundancy of 16S rRNA sequences in genomes with multiple rrn operons. J Bacteriol 186 (2004) 2629-2635
3. Barry T., Colleran G., Glennon M., Duncan L.K., and Gannon F. The 16S-23S ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Methods Appl 1 (1991) 51-56
4. Blaiotta G., Fusco V., Ercolini D., Aponte M., Pepe O., and Villani F. Diversity of Lactobacillus strains based on partial HSP60 gene sequences and design of PCR-RFLP assays for species identification and differentiation. Appl Environ Microbiol 74 1 (2008) 208-215
5. Chiang Y.C., Liao W.W., Fan C.M., Pai W.Y., Chiou C.S., and Tsen H.Y. PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. Int J Food Microbiol 121 1 (2008) 66-73
6. Chiang Y.C., Fan C.M., Liao W.W., Lin C.K., and Tsen H.Y. Real-Time PCR detection of Staphylococcus aureus in milk and meat using new primers designed from the heat shock protein gene htrA sequence. J. Food Prot 70 12 (2007) 2855-2859
7. Daly P., Collier T., and Doyle S. PCR-ELISA detection of Escherichia coli in milk. Lett Appl Microbiol 34 (2002) 222-226
8. Dobson C.M., Deneer H., Lee S., Hemmingsen S., Glaze S., and Ziola B. Phylogenetic analysis of the genus Pediococcus, including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. Int J Syst Evol Microbiol 52 (2002) 2003-2010
9. Food and Drug Administration. Bacteriological analytical manual. 8th ed. (1995), AOAC International, USA
10. Forsman P., Tilsal-Timisjarvi A., and Alatossava T. Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions. Microbiology 143 (1997) 3491-3500
11. Fuchs B.M., Syutsubo K., Ludwig W., and Amann R. In situ accessibility of Escherichia coli 23S rRNA to fluorescently labeled oligonucleotide probes. Appl Environ Microbiol 67 2 (2001) 961-968
12. Gillespie B.E., and Oliver S.P. Simultaneous detection of mastitis pathogens, Staphylococcus aureus, Streptococcus uberis, and Streptococcus agalactiae by multiplex real-time polymerase chain reaction. J Dairy Sci 88 10 (2005) 3510-3518
13. Goh S.H., Potter S., Wood J.O., Hemmingsen S.M., Reynolds R.P., and Chow A.W. HSP60 gene sequences as universal target for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol 34 4 (1996) 818-823
14. Goh S.H., Driedger D., Gillett S., Low D.E., Hemmingsen S.M., Amos M., et al. Streptococcus iniae, a human and animal pathogen: specific identification by the chaperonin 60 gene identification method. J Clin Microbiol 36 7 (1998) 2164-2166
15. Goh S.H., Facklam R.R., Chang M., Hill J.E., Tyrrell G.J., Burns E.C., et al. Identification of Enterococcus species and phenotypically similar Lactococcus and Vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. J Clin Microbiol 38 11 (2000) 3953-3959
16. Goh S.H., Santucci Z., Kloos W.E., Faltyn M., George C.G., Driedger D., et al. Identification of Staphylococcus species and subspecies by the chaperonin 60 gene identification method and reverse checkerboard hybridization. J Clin Microbiol 35 12 (1997) 3116-3121
17. Gurtler V., and Stanisich V.A. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142 (1996) 3-16
18. Jayarao B.M., Gillespie B.E., and Oliver S.P. Application of randomly amplified polymorphic DNA fingerprinting for species identification of bacteria isolated from bovine milk. J. Food Prot 59 (1996) 615-620
19. Jensen M.A., Webster J.A., and Straus N. Rapid identification of bacteria on the basis of polymerase chain reaction amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol 3 (1993) 186-194
20. Kwok A.Y., and Chow A.W. Phylogenetic study of Staphylococcus and Macrococcus species based on partial hsp60 gene sequence. Int J Syst Evol Microbiol 53 (2003) 87-92
21. Kwok A.Y., Su S.C., Reynolds R.P., Bay S.J., Av-Gay Y., Dovichi N.J., et al. Species identification and phylogenetic relationships based on partial HSP60 gene sequences within the genus Staphylococcus. Int. J. Syst. Bacteriol 49 (1999) 1181-1192
22. Lin C.K., Hung C.L., Chiang Y.C., and Tsen H.Y. The sequence heterogenicities among 16S rRNA genes of Salmonella serovars and the effects on the specificity of the primers designed. Int J Food Microbiol 96 (2004) 205-214
23. Maidak B.L., Cole J.R., Lilburn T.G., Parker Jr. C.T., Saxman P.R., Stredwick J.M., et al. The RDP (Ribosomal Database Project) continues. Nucleic Acids Res 28 1 (2000) 173-174
24. McDonald W.L., Fry B.N., and Deighton M.A. Identification of Streptococcus spp. causing bovine mastitis by PCR-RFLP of 16S-23S ribosomal DNA. Vet Microbiol. 111 3-4 (2005) 241-246
25. Myllys V., Honkanen-Buzalski T., Virtanen H., Pyörälä S., and Müller H.P. Effect of abrasion of teat orifice epithelium on development of bovine staphylococcal mastitis. J Dairy Sci 77 2 (1994) 446-452
26. Phuektes P., Browning G.R., Anderson G., and Mansell P.D. Multiplex polymerase chain reaction as a mastitis screening test for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis. J Dairy Res 70 (2003) 149-155
27. Phuektes P., Mansell P.D., and Browning G.F. Multiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and Streptococcal causes of bovine mastitis. J Dairy Sci 84 (2001) 1140-1148
28. Riffon R., Sayasith K., Khalil H., Dubreuil P., Drolet M., and Lagace J. Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J Clin Microbiol 39 (2001) 2584-2589
29. Teng L.J., Hsueh P.R., Wang Y.H., Lin H.M., Luh K.T., and Ho H.M. Determination of Enterococcus faecalis groESL full length sequence and application for species identification. J Clin Microbiol 39 (2001) 3326-3331
30. Wong R.S., and Chow A.W. Identification of enteric pathogens by heat shock protein 60 kDa (HSP60) gene sequences. FEMS Microbiol. Lett. 206 (2002) 107-113
31. Zhu L., Li W., and Dong X. Species identification of genus Bifidobacterium based on partial HSP60 gene sequences and proposal of Bifidobacterium thermacidophilum subsp. porcinum subsp. nov. Int J Syst Evol Microbiol 53 (2003) 1619-1623