Reduced expression of PNUTS leads to activation of Rb-phosphatase and caspase-mediated apoptosis

Cancer Biology and Therapy

Volumn 7, Issue 6, 2008, Pages 833-841

  De Leon, Gabriel ^a   Sherry, Tara C ^a   Krucher, Nancy A ^a,b  

^a PACE UNIVERSITY   (United States)

^b NONE   (United States)

Author keywords

Apoptosis; p53; PNUTS; PP1; Rb

Indexed keywords

CASPASE 8; CASPASE 8 INHIBITOR; CASPASE 9; CASPASE 9 INHIBITOR; PHOSPHOPROTEIN PHOSPHATASE 1; PROTEIN P53; PROTEIN PNUTS; RETINOBLASTOMA PROTEIN; CASPASE; DNA BINDING PROTEIN; NUCLEAR PROTEIN; PPP1R10 PROTEIN, HUMAN; RNA BINDING PROTEIN;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL LYSATE; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; ANOXIA; BIOSYNTHESIS; CELL SURVIVAL; ENZYME ACTIVATION; GENE EXPRESSION REGULATION; METABOLISM; PHOSPHORYLATION; RNA INTERFERENCE; TUMOR CELL LINE;

ANOXIA; APOPTOSIS; CASPASE 8; CASPASES; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; DNA-BINDING PROTEINS; ENZYME ACTIVATION; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; NUCLEAR PROTEINS; PHOSPHORYLATION; RETINOBLASTOMA PROTEIN; RNA INTERFERENCE; RNA-BINDING PROTEINS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 46749089975     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.7.6.5839     Document Type: Article

References (57)

1. Classon M and Harlow E. The retinoblastoma tumor suppressor in development and cancer. Nat Rev Cancer 2002; 2:910-7.
2. Sherr CJ and McCormick F. The RB and p53 pathways in cancer. Cancer Cell 2002; 2:103-12.
3. Knudsen ES and Wang JYJ. Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase mediated RB phosphorylation. Mol Cell Bio 1997; 17:5771-83.
4. 1 cyclin/cyclin dependent kiase coordinated phosphorylation of endogenous pocket proteins differentially regulates their interaction with E2F4 and E2F1 and gene expression. J Biol Chem 2002; 277:50263-74.
5. Ludlow JW, Glendening CL, Livingston DM and DeCaprario JA. Specific Enzymatic dephosphorylation of the retinoblastoma protein. Mol Cell Biol 1993; 13:367-72.
6. Nelson DA, Krucher NA and Ludlow JW. High molecular weight protein phosphatase type 1 dephosphorylates the retinoblastoma protein. J Biol Chem 1997; 272:4528-35.
7. Berndt N. Roles and regulation of serine/threonine-specific protein phosphatases in the cell cycle. Prog Cell Cycle Res 2003; 5:497-510.
8. Cohen PT. Protein Phosphatase 1- targeted in many directions. J Cell Sci 2002; 115:241-56.
9. Kreivi JP, Trinkle Mulcahy L, Lyon CE, Morrice NA, Cohen P and Lamond AI. Purification and characterization of p99, a nuclear modulator of protein phosphatase 1 activity. FEBS Lett 1997; 420:57-62.
10. Allen PB, Kwon YG, Nairn AC and Greengard P. Isolation and characterization of PNUTS, a putative protein phosphatase 1 nuclear targeting subunit. J Biol Chem 1998; 273:4089-95.
11. Kim YM, Watanabe T, Allen PB, Kim YM, Lee SJ, Greengard P, Nairn AC and Kwon YG. PNUTS, a protein phosphatase 1 (PP1) Targeting Subunit. Characterization of its PP1 and RNA binding domains and regulation by phosphorylation. J Biol Chem 2003; 278:13819-28.
12. Krucher NA, Rubin E, Tedesco VC, Roberts MH, Sherry TC, De Leon G. Dephosphorylation of Rb (Thr-821) in response to cell stress. Exp Cell Res 2006; 312:2757-63.
13. Udho E, Tedesco VC, Zygmunt A, Krucher NA. PNUTS (Phosphatase Nuclear Targeting Subunit) inhibits retinoblastoma-directed PP1 activity. Biochem Biophys Res Comm 2002; 297:463-7.
14. Clarke AR, Maandag ER, Van Roon M, Van De Lugt NMT, Van Der Valk M, Hooper ML, Berns A, Rielef HT. Requirement for a functional Rb-1 gene in murine development. Nature 1992; 359:328-30.
15. Jacks T, Fazeli A, Schmitt EM, Bronson RT, Goodell MA, Weinberg RA. Effects of an Rb mutation in the mouse. Nature 1992; 359:295-300.
16. Lee EYHP, Chang CY, Hu N, Wang YCJ, Lai CC, Herrup K, Lee WH, Bradley A. Mice deficient for Rb are nonviable and show defects in neurogenesis and haematopoiesis. Nature 1992; 359:288-94.
17. Haas Kogan D, Kogan SC, Levi D, Dazin P, T'Ang A, Fung YKT, Isreal MA. Inhibition of apoptosis by retinoblastoma gene product. EMBO J 1995; 14:461-72.
18. Tsai KY, Hu Y, Macleod KF, Crowley D, Yamasaki L, Jacks T. Mutation of E2F1 suppresses apoptosis and inappropriate S phase entry and extends survival of Rb-deficient mouse embryos. Mol Cell 1998; 2:293-304.
19. Hsieh JK, Fredersdorf S, Kouzarides T, Martin K, Lu X. E2F1-induced apoptosis requires DNA binding but not transactivation and is inhibited by the retinoblastoma protein through direct interaction. Genes Dev 1997; 11:1840-52.
20. Phillips AC, Ernst MK, Bates S, Rice NR, Vousden KH. E2F1 potentiates cell death by blocking antiapoptotic signaling pathways. Mol Cell 1999; 4:771-81.
21. Wells J, Boyd KE, Fry CJ, Bartley SM, Farnham PJ. Target gene specificity of E2F and pocket protein family members in living cells. Mol Cell Bio 2000; 20:5797-807.
22. Nahle Z, Polakoff J, Davuluri RV, McCurrach ME, Jacobson MD, Narita M, Zhang MQ, Lazebnik Y, Bar-Sagi D. Lowe SW. Direct coupling of the cell cycle and cell death machinery by E2F. Nat Cell Bio 2002; 4:859-64.
23. Rofstad EK, Sutherland RM. Radiation sensitivity of human ovarian carcinoma cell lines in vitro:effects of growth factors, basement membrane and intercellular contact. Int J Radiation Oncology Biol Phys 1988; 15:921-9.
24. 1 restraint and to tumorigenesis by haploid insufficiency. Mol Cell Bio 2005; 25:3535-42.
25. Krucher NA, Zygmunt A, Mazloum N, Tamrakar S, Ludlow JW, Lee MYWT. Interaction of the retinoblastoma protein (pRb) with the catalytic subunit of DNA polymerase delta (p125). Oncogene 2000; 19:5464-70.
26. Martin DA, Siegel RM, Zheng L, Lenardo MJ. Membrane oligomerization and cleavage activates the caspase-8 (FLICE/MACHa1) death signal. J Biol Chem 1998; 273:4345-9.
27. Fadeel B, Hassan Z, Hellstrom Lindberg E, Henter JI, Orrenius S, Zhivotovsky. Cleavage of bcl-2 is an early event in chemotherapy-induced apoptosis of human myeloid leukemia cells. Leukemia 1999; 13:719-28.
28. Roach P, Roach PJ, DePaoli Roach AA. Phosphoprotein phosphatase inhibitor-2 Identification as a species of molecular weight 31,000 in rabbit muscle, liver and other tissues. J Biol Chem 1985; 260:6314-7.
29. Ingebritsen TS and Cohen P. The protein phosphatases involved in cellular regulation. 1 Classification of substrate specificities. Eur J Biochem 1983; 132:255-61.
30. Cohen P. Classification of protein serine/threonine phosphatases: identification and quantification in methods in cell extracts. Methods in Enzymology 1991; 201:389-98.
31. Ludlow JW, Nelson DA and Krucher NA. Use of immunocomplexed substrate for detecting PP1 activity. Methods Mol Biol 1998; 93:137-43.
32. Lee SJ, Lim CJ, Min JK, Lee YM, Lee JY, Won MH, Kwon YG. Protein phosphatase 1 nuclear targeting subunit is a hypoxia inducible gene: its role in post-translational modification of p53 and MDM2. Cell Death and Diff 2007; 14:1106-16.
33. Soule HD, Maloney TM, Wolman SR, Peterson Jr WD, Brenz R, McGrath CM, Russo J, Pauley RJ, Jones RF and Brooks SC. Isolation and characterization of a spontaneously immortalized human breast epithelial cell MCF10. Cancer Res 1990; 50:6075-60.
34. Wolf BB and Green DR. Suicidal Tendencies: Apoptotic Cell Death by caspase family proteinases. J Biol Chem 1999; 274:20049-52.
35. Shew JY, Lin B, Chen PL, Tseng BY, Yang Feng TL, Lee WH. C-terminal truncation of the retinoblastoma gene product leads to functional inactivation. Proc Natl Acad Sci USA 1990; 87:6-10.
36. 1 phase of the cell cycle. Cell 1991; 67:293-302.
37. 2 arrest after DNA damage. Science 1998; 282:1497-501.
38. Wu GS and Ding Z. Caspase 9 is required for p53-dependent apoptosis and chemosensitivity in a human ovarian cancer cell line. Oncogene 2002; 21:1-8.
39. Brown V, Phillips RA, Gallie BL. Cumulative effect of phosphorylation of pRB on regulation of E2F activity. Mol Cell Bio 1999; 19:3246-56.
40. Afshar G, Jelluma N, Yang X, Basila D, Arvold ND, Karlsson A, Yount GL, Dansen TB, Koller E, Haas Kogan DA. Radiation-induced caspase-8 mediates p53 independent apoptosis in glioma cells. Cancer Res 2006; 66:4223-32.
41. Putzer BM, Stiewe T, Parssanedjad K, Rega S, Esche H. E1A is sufficient by itself to induce apoptosis independent of p53 and other adenoviral gene products. Cell Death and Diff 2000; 7:177-88.
42. 1 arrest in human cancer cells. Current Biology 1997; 7:375-86.
43. 1 arrest and apoptosis. Proc Natl Acad Sci USA 1995; 92:9019-23.
44. Morana SJ, Wolf CM, Li J, Reynolds JE, Brown MK, Eastman A. The involvement of protein phosphatases in the activation of ICE/CED-3 protease, intracellular acidification, DNA digestion, and apoptosis. J Biol Chem 1996; 271:18263-71.
45. Garriga J, Jayaraman AL, Limon A, Jayadeva G, Sotillo E, Truongcao M, Patsialou A, Wadzinski BE, Grana X. A Dynamic equilibrium between cdks and PP2A modulates phosphorylation of pRb, p107 and p130. Cell Cycle 2003; 3:1320-30.
46. Cicchillitti L, Fasanaro P, Biglioli P, Capogrossi MC, Martelli F. Oxidative stress induces protein phosphatase 2A-dependent dephosphorylation of the procket proteins pRb, p107 and p130. J Biol Chem 2003; 278:18517-9509.
47. Magenta A, Fasanaro P, Romani S, Di Stefano V, Capogrossi MC, Martelli F. Protein phosphatase 2A subunit PR70 interacts with pRb and mediates its dephosphorylation. Mol Cell Biol 2008; 28:873-82.
48. Muller H, Bracken AP, Vernell R, Moroni MC, Christians F, Grassilli E, Prosperini E, Vigo E, Oliner JD, Helin K. E2Fs regulate the expression of genes involved in differentiation, development, proliferation and apoptosis. Genes Dev 2001; 15:267-85.
49. Degregori J, Leone G, Miron A, Jakoi L, Nevins JR. Distinct roles for E2F proteins in cell growth control and apoptosis. Proc. Natl. Acad. Sci USA 1997; 94:7245-50.
50. Denchi EL, Helin K. E2F1 is crucial for E2F-dependent apoptosis. EMBO Reports 2005; 6:661-7.
51. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science 1998; 281:1312-6.
52. Shu HKG, Julin CM, Furman F, Yount GL, Haas Kogan D, Isreal MA. Overexpression of E2F1 in glioma derived cell lines induces a p53-independent apoptosis that is furthered enhanced by ionizing radiation. Neuro-Oncology 2000; 2:16-21.
53. Lieman JH, Worley LA, Harbour JW. Loss of Rb-E2F repression results in caspase-8 mediated apoptosis through inactivation of focal adhesion kinase. J Biol Chem 2005; 280:10484-90.
54. Whitlock JA, Krailo M, Reid JM, Ruben SL, Ames MM, Owen W, Reaman G. Phase 1 clinical and pharmacokinetic study of flavopiridol in children with refractory solid tumors: a childrens oncology group study. J Clin Oncology 2005; 23:9179-86.
55. El-Rayes BF, Gadgeel S, Parchment R, Lorusso P, Philip PA. A phase 1 study of flavopiridol and docetaxel. Invest. New Drugs 2006; 24:305-10.
56. Benson C, White J, De Bono J, O'Donnell A, Raynaud F, Cruickshank C, McGrath H, Walton M, Workman P, Kaye S, Cassidy J, Gianella Borradori A, Judson I, Twleves C. A phase 1 trail of the selective oral cyclin-dependent kinase inhibitor seliciclib (cyc 201, Rroscovitine) administered twice daily for 7 days every 21 days. Br J Cancer 2007; 96:29-37.
57. Vogelstein B, Fearon ER, Kern SE, Hamilton SR, Preisinger AC, Nakamura Y, White R. Allelotype of colorectal carcinomas. Science 1989; 244:207-11.