A novel method for the detection of viable human pancreatic beta cells by flow cytometry using fluorophores that selectively detect labile zinc, mitochondrial membrane potential and protein thiols

Cytometry Part A

Volumn 73, Issue 7, 2008, Pages 615-625

  Jayaraman, Sundararajan ^a  

^a UNIVERSITY OF ILLINOIS   (United States)

Author keywords

Beta cells; Caspases; Glutathione; Labile zinc; Mitochondrial membrane potential; Thiols; Vital dyes

Indexed keywords

CHELATING AGENT; DYE; FLUOZIN 3 ACETOXYMETHYL ESTER; MONOCHLOROBIMANE; RHODAMINE; TETRAMETHYLRHODAMINE ETHYL ESTER; THIOL; ZINC;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BINDING COMPETITION; CELL FUNCTION; CELL VIABILITY; CONTROLLED STUDY; FIBROBLAST; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INSULIN DEPENDENT DIABETES MELLITUS; LYMPHOID CELL; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; MOUSE; NONHUMAN; OXIDATIVE STRESS; PANCREAS ISLET BETA CELL; PANCREAS ISLET TRANSPLANTATION; PRIORITY JOURNAL; RAT; STAINING; VALIDATION PROCESS;

ANIMALS; CELL LINE; CELL SEPARATION; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; INSULIN-SECRETING CELLS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MICE; MITOCHONDRIAL MEMBRANES; PROTEINS; SULFHYDRYL COMPOUNDS; ZINC;

EID: 46449104381     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20560     Document Type: Article

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