Identification and purification of functional human β-cells by a new specific zinc-fluorescent probe

Journal of Histochemistry and Cytochemistry

Volumn 49, Issue 4, 2001, Pages 519-527

  Lukowiak, B ^a   Vandewalle, B ^a   Riachy, R ^a   Kerr Conte, J ^a   Gmyr, V ^a   Belaich, S ^a   Lefebvre, J ^a   Pattou, F ^a  

^a UNIV LILLE   (France)

Author keywords

Fluorescence activated cell; Human pancreatic islet; Human pancreatic cell; Identification; Purification; Sorting; Zinc sensitive fluorescent probe

Indexed keywords

DNA; FLUORESCENT DYE; INSULIN; NEWPORT GREEN; UNCLASSIFIED DRUG; ZINC;

ADULT; ARTICLE; CELL SELECTION; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DNA DETERMINATION; FLUORESCENCE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; REGRESSION ANALYSIS; SECRETORY GRANULE;

EID: 0035070655     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540104900412     Document Type: Article

References (19)

1. Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines
2. Three-dimensional imaging of intact isolated islets of Langerhans with confocal microscopy
3. Imaging free zinc in synaptic terminals in live hippocampal slices
4. Measurement of intracellular free zinc in living neurons
5. Beta-cell line derived from transgenic mice expressing a hybrid insulin gene-oncogene
6. Zinc and endogenous zinc content and distribution in islets in relationship to insulin content
7. Fluorescent indicators for Zn2+ and other metals
8. Zinc-specific N-(6-methoxy-8-quinolyl)-para-toluenesulfonamide as a selective nontoxic fluorescence stain for pancreatic islets
9. Simple dithizone-stained multilayer test for selection of density, gradient before human islet mass purification
10. Large-scale purification of human islets utilizing discontinuous albumin gradient on IBM 2991 cell separator
11. Variables in organ donors that affect the recovery of human islets of Langerhans
12. A simple method of staining fresh and cultured islets
13. Sorting human B-cells consequent to targeted expression of green fluorescent protein
14. Islet isolation assessment in man and large animals
15. Automated method for isolation of human pancreatic islets
16. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen
17. Fixation of ejaculated spermatozoa for electron microscopy
18. Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin [(2-methyl-8-p-toluenesulphoamido-6-quinolyloxy)acetic acid], a new specific fluorescent probe for Zn(II)
19. Video image analysis of labile zinc in viable pancreatic islet cells using a specific fluorescent probe for zinc