Chapter 8 Immunogold Labeling of Thawed Cryosections

Methods in Cell Biology

Volumn 88, Issue , 2008, Pages 131-149

  Peters, Peter J ^a   Pierson, Jason ^a  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDE; BIOMATERIAL; CARBON; FIXATIVE; POLYVINYL ACETATE; POLYVINYL DERIVATIVE;

ANIMAL; CHEMISTRY; HISTOLOGY; HUMAN; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; METHODOLOGY; REVIEW; ULTRAMICROTOMY;

ALDEHYDES; ANIMALS; CARBON; COATED MATERIALS, BIOCOMPATIBLE; CRYOULTRAMICROTOMY; FIXATIVES; HUMANS; IMMUNOHISTOCHEMISTRY; POLYVINYLS; TISSUE EMBEDDING;

EID: 46449098867     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0091-679X(08)00408-1     Document Type: Review

References (26)

1. Al-Amoudi A., Chang J.J., Leforestier A., McDowall A., Salamin L.-A., Norlén L.P.O., Richter K., Sartori Blanc N., Studer D., and Dubochet J. Cryo-electron microscopy of vitreous sections. EMBO J. 23 (2004) 3583-3588
2. Al-Amoudi A., Studer D., and Dubochet J. Cutting artifacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150 (2005) 109-121
3. Aurion. Optimised immuno labeling using Aurion Blocking Solutions and Aurion BSA-c™. Technical Support: Newsletters & Newsflyers (2006) Newsletter 1
4. Bouchet-Marquis C., Zuber B., Glynn A.M., Eltsov M., Grabenbauer M., Goldie K.N., Thomas D., Frangakis A.S., Dubochet J., and Chrétien D. Visualization of cell microtubules in their native state. Biol. Cell 99 (2007)
5. Christensen A.K. Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver. J. Cell Biol. 51 (1971) 772-804
6. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., McDowall A.W., and Schultz P. Cryo-electron microscopy of vitrified specimens. Q Rev Biophys. 21 (1988) 129-228
7. Dubochet J., McDowall A.W., Menge B., Schmid E.N., and Lickfeld K.G. Electron microscopy of frozen-hydrated bacteria. J. Bacteriol. 155 (1983) 381-390
8. Faulk W.P., and Taylor G.M. An immunocolloid method for the electron microscope. Immunochemistry. 8 11 (1971) 1081-1083
9. Frederik P.M., Busing W.M., and Hax W.M. Frozen-hydrated and drying thin cryo-sections observed in STEM. J. Microsc. 126 Pt 1 (1982) RP1-RP2
10. Hsieh C.E., Leith A., Mannella C.A., Frank J., and Marko M. Towards high-resolution three-dimensional imaging of native mammalian tissue: Electron tomography of frozen-hydrated rat liver sections. J. Struct. Biol. 153 (2006) 1-13
11. Koster A.J., Chen H., Sedat J.W., and Agard D.A. Automated microscopy for electron tomography. Ultramicroscopy 46 (1992) 207-227
12. Kremer J.R., Mastronarde D.N., and McIntosh J.R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116 (1996) 71-76
13. Ladinsky M.S., and Howell K.E. Electron Tomography of Immunolabeled Cryosections. In: McIntosh J.R. (Ed). Cellular Electron Microscopy Vol. 79 (2007), Elsevier, California 543-558
14. Marko M., Hsieh C.H., and Mannella C.A. Electron Tomography of Frozen-hydrated Sections of Cells and Tissues. In: Frank J. (Ed). Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell. 2nd edn (2007), Springer, New York 49-81
15. McDowall A.W., Chang J.J., Freeman R., Lepault J., Walter C.A., and Dubochet J. Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples. J. Microscopy 131 (1983) 1-9.
16. Mironov A., Latawiec D., Wille H., Bouzamondo-Bernstein E., Legname G., Williamson R.A., Burton D., DeArmond S.J., Prusiner S.B., and Peters P.J. Cytosolic Prion Proteins in Neurons. J. Neurosci. 23 (2003) 7183-7193
17. Nakane P.K., and Pierce G.B. Enzyme-labeled antibodies for light and electron microscopic localization of tissue antigens. J. Cell Biol. 33 (1967) 308-318
18. Nicastro D., Schwartz C., Pierson J., Gaudette R., Porter M.E., and McIntosh J.R. Cryo-tomography of axonemes: Insights into the regulation of dynein activity and microtubule stability. Science 313 (2006) 944-948
19. Raposo G., Kleijmeer M.J., Posthuma G., Slot J.W., and Geuze H.J. Immunogold labeling of ultrathin cryosections: Application in immunology. In: Cambridge M.A., Herzenberg L.A., Weir D., Herzenberg L.A., and Blackwell C. (Eds). Handbook of Experimental Immunology. 5th edn. Vol. 4 (1997), Blackwell, Oxford 208 1-11
20. Singer S.J. Preparation of an electron-dense antibody conjugate. Nature 183 (1959) 1523-1525
21. Sternberger L.A., Hardy P.H.Jr., Cuculis J.J., and Meyer H.G. The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antibody-antigen complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem. Cytochem 18 (1970) 315-333
22. Studer D., Michel M., and Muller M. High pressure freezing comes of age. Scanning Microsc. 53 (1989) 253-269
23. Tokuyasu K.T. A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57 (1973) 551-565
24. van der Wel N.N., Fluitsma D.M., Dascher C.C., Brenner M.B., and Peters P.J. Subcellular localization of mycobacteria in tissues and detection of lipid antigens in organelles using cryo-techniques for light and electron microscopy. Curr. Opin. Microsc. 8 (2005) 323-330
25. Zeuschner D., Geerts W.J.C., Donselaar E.V., Humbel B.M., Slot J.W., Koster A.J., and Klumperman J. Immuno-electron tomography of ER exit sites reveals the existence of free COPII-coated transport carriers. Nat. Cell Biol. 8 (2006) 377-383
26. Zhang P., Bos E., Heymann J., Gnaegi H., Kessel M., Peters P.J., and Subramaniam S. Direct visualization of receptor arrays in frozen-hydrated sections and plunge-frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr. J. Microsc. 216 (2004) 76-83