Gene expression during fracture healing in rats comparing intramedullary fixation to plate fixation by DNA microarray

Journal of Orthopaedic Trauma

Volumn 20, Issue 1, 2006, Pages 27-38

  Heiner, Daniel E ^a   Meyer, Martha H ^a   Frick, Steven L ^a   Kellam, James F ^a   Fiechtl, James ^a   Meyer Jr , Ralph A ^a  

^a CAROLINAS MEDICAL CENTER   (United States)

Author keywords

Femur; Fracture; Microarray; Nail; Plate; Rat

Indexed keywords

ACID PHOSPHATASE TARTRATE RESISTANT ISOENZYME; AGGRECAN; BIGLYCAN; BONE MORPHOGENETIC PROTEIN; CARBOXYPEPTIDASE A; CARTILAGE OLIGOMERIC MATRIX PROTEIN; CHOLINERGIC RECEPTOR; CHYMASE; COLLAGEN TYPE 1; COLLAGEN TYPE 3; COLLAGEN TYPE 5; COMPLEMENTARY DNA; CYCLIN D2; CYCLIN DEPENDENT KINASE 2; CYCLOOXYGENASE 2; CYTOKINE; DEFENSIN; GALANIN; MACROPHAGE ELASTASE; MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA; MESSENGER RNA; PROTEIN C FOS; PROTEIN KINASE FYN; PROTEIN LYSINE 6 OXIDASE; RIBOSOME PROTEIN; SOMATOMEDIN BINDING PROTEIN 5; SOMATOMEDIN C; SUPPRESSOR OF CYTOKINE SIGNALING 3; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 2; TRANSFORMING GROWTH FACTOR BETA3;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BONE PLATE; BONE SCREW; CELL TYPE; CONTROLLED STUDY; FEMALE; FEMUR FRACTURE; FRACTURE FIXATION; FRACTURE HEALING; FRACTURE TREATMENT; GENE EXPRESSION REGULATION; INTERMETHOD COMPARISON; INTRAMEDULLARY NAILING; MAST CELL; MICROARRAY ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLATE FIXATION; PRIORITY JOURNAL; PROTO ONCOGENE; RAT; ANIMAL; CALLUS; COMPARATIVE STUDY; DNA MICROARRAY; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; PHYSIOLOGY; SPRAGUE DAWLEY RAT; UPREGULATION;

ANIMALS; BONE PLATES; BONY CALLUS; FEMALE; FEMORAL FRACTURES; FRACTURE FIXATION, INTRAMEDULLARY; FRACTURE HEALING; GENE EXPRESSION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; TRANSCRIPTION, GENETIC; UP-REGULATION;

EID: 33646843614     PISSN: 08905339     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.bot.0000184143.90448.aa     Document Type: Article

References (54)

1. Gerstenfeld LC, Einhorn TA. Developmental aspects of fracture healing and the use of pharmacological agents to alter healing. J Musculoskelet Neuronal Interact. 2003;3:297-303.
2. Cullinane DM, Salisbury KT, Alkhiary Y, et al. Effects of the local mechanical environment on vertebrate tissue differentiation during repair: does repair recapitulate development? J Exp Biol. 2003;206:2459-2471.
3. Le AX, Miclau T, Hu D, et al. Molecular aspects of healing in stabilized and non-stabilized fractures. J Orthop Res. 2001;19:78-84.
4. Hankemeier S, Grässel S, Plenz G, et al. Alteration of fracture stability influences chondrogenesis, osteogenesis and immigration of macrophages. J Orthop Res. 2001;19:531-538.
5. Lipshutz RJ, Fodor SPA, Gingeras TR, et al. High density synthetic oligonucleotide arrays. Nat Genet. 1999;21(Suppl.):20-24.
6. Freeman WM, Robertson DJ, Vrana KE. Fundamentals of DNA hybridization arrays for gene expression analysis. Biotechniques. 2000;29:1042-1055.
7. Meyer RA Jr, Tsahakis PJ, Martin DF, et al. Age and ovariectomy impair both the normalization of mechanical properties and the accretion of mineral by the fracture callus in rats. J Orthop Res. 2001;19:428-435.
8. Meyer RA Jr, Meyer MH, Tenholder M, et al. Gene expression in older rats with delayed union of femoral fractures. J Bone Joint Surg Am. 2003;85A:1243-1254.
9. Gardner TN, Hardy J, Evans M, et al. Temporal changes in dynamic inter fragmentary motion and callus formation in fractures. J Biomech. 1997;30:315-321.
10. Zar JH. Biostatistical Analysis. Englewood Cliffs: Prentice-Hall, Inc.; 1984.
11. Meyer MH, Etienne W, Meyer RA Jr. Altered mRNA expression of genes related to nerve cell activity in the fracture callus of older rats: a randomized, controlled, microarray study. BMC Musculoskelet Disord. 2004;5:24.
12. Hadjiargyrou M, Lombardo F, Zhao S, et al. Transcriptional profiling of bone regeneration. Insight into the molecular complexity of wound repair. J Biol Chem. 2002;277:30177-30182.
13. Sims NA, Jenkins BJ, Quinn JM, et al. Glycoprotein 130 regulates bone turnover and bone size by distinct downstream signaling pathways. J Clin Invest. 2004;113:379-389.
14. Shin HI, Divieti P, Sims NA, et al. Gp130-mediated signaling is necessary for normal osteoblastic function in vivo and in vitro. Endocrinology. 2004;145:1376-1385.
15. Lang R, Pauleau AL, Parganas E, et al. SOCS3 regulates the plasticity of gp130 signaling. Nat Immunol. 2003;4:546-550.
16. Starr R, Willson TA, Viney EM, et al. A family of cytokine-inducible inhibitors of signaling. Nature. 1997;387:917-921.
17. Cuesta R, Laroia G, Schneider RJ. Chaperone hsp27 inhibits translation during heat shock by binding eIF4G and facilitating dissociation of capinitiation complexes. Genes Dev. 2000;14:1460-1470.
18. Billiard J, Moran RA, Whitley MZ, et al. Transcriptional profiling of human osteoblast differentiation. J Cell Biochem. 2003;89:389-400.
19. Einhorn TA. Cox-2: Where are we in 2003? The role of cyclooxygenase-2 in bone repair. Arthritis Res Ther. 2003;5:5-7.
20. Lian JB, Stein GS, Aubin JE. Bone formation: maturation and functional activities of osteoblast lineage cells. In: Favus MJ, ed. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. Washington, D.C.: American Society for Bone and Mineral Research; 2003:13-28.
21. Canalis E. Osteogenic growth factors. In: Favus MJ, ed. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. Washington, D.C.: American Society for Bone and Mineral Research; 2003:28-31.
22. Meyer RA Jr, Meyer MH, Phieffer LS, et al. Delayed union of femoral fractures in older rats: decreased gene expression. BMC Musculoskelet Disord. 2001;2:2.
23. Desai BJ, Meyer MH, Porter S, et al. The effect of age on gene expression in adult and juvenile rats following femoral fracture. J Orthop Trauma. 2003;17:689-698.
24. Robey PG, Boskey AL. Extracellular matrix and biomineralization of bone. In: Favus MJ, ed. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. Washington, D.C.: American Society for Bone and Mineral Research; 2003:38-46.
25. Bland YS, Critchlow MA, Ashhurst DE. The expression of the fibrillar collagen genes during fracture healing: Heterogeneity of the matrices and differentiation of the osteoprogenitor cells. Histochem J. 1999;31:797-809.
26. Yamazaki M, Majeska RJ, Yoshioka H, et al. Spatial and temporal expression of fibril-forming minor collagen genes (types Vand XI) during fracture healing. J Orthop Res. 1997;15:757-764.
27. Benjamin M, Ralphs JR. Biology of fibrocartilage cells. Int Rev Cytol. 2004;233:1-45.
28. Etienne W, Meyer MH, Peppers J, et al. Comparison of mRNA gene expression by RT-PCR and DNA microarray. Biotechniques. 2004;36:618-626.
29. Doty SB. Histochemistry and enzymology of osteoclasts. In: Hall BK, ed. Bone, Volume 2: The Osteoclast. Boca Raton: CRC Press; 1991:61-85.
30. Ashhurst DE. Macromolecular synthesis and mechanical stability during fracture repair. In: Hall BK, ed. Bone, Volume 5: Fracture Repair and Regeneration. Boca Raton: CRC Press; 1992:61-121.
31. Issack PS, Liu C, Prazak L, et al. A silencer element in the cartilage oligomeric matrix protein gene regulates chondrocyte-specific expression. J Orthop Res. 2004;22:751-758.
32. Di Cesare PE, Chen FS, Moergelin M, et al. Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin. Matrix Biol. 2002;21:461-470.
33. DiCesare PE, Morgelin M, Mann K, et al. Cartilage oligomeric matrix protein and thrombospondin 1. Purification from articular cartilage, electron microscopic structure, and chondrocyte binding. Eur J Biochem. 1994;223:927-937.
34. Mizuno M, Fujisawa R, Kuboki Y. Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and shows affinity to collagen. Calcif Tissue Int. 1996;59:163-167.
35. Wang ZQ, Ovitt C, Grigoriadis AE, et al. Bone and haematopoietic defects in mice lacking c-fos. Nature. 1992;360:741-745.
36. Chen Q, Pearlman RE, Moens PB. Isolation and characterization of a cDNA encoding a synaptonemal complex protein. Biochem Cell Biol. 1992;70:1030-1038.
37. Ochs RL, Stein TW Jr, Chan EK, et al. cDNA cloning and characterization of a novel nucleolar protein. Mol Biol Cell. 1996;7:1015-1024.
38. Bouchard C, Thieke K, Maier A, et al. Direct induction of cyclin D2 by Myc contributes to cell cycle progression and sequestration of p27. EMBO J. 1999;18:5321-5333.
39. Kotani S, Endo T, Kitagawa M, et al. A variant form of cyclin-dependent kinase 2 (Cdk2) in a malignantly transformed rat thyroid (FRTL-Tc) cell line. Oncogene. 1995;10:663-669.
40. Kaabeche K, Lemonnier J, Le Mee S, et al. Cbl-mediated degradation of Lyn and Fyn induced by constitutive fibroblast growth factor receptor-2 activation supports osteoblast differentiation. J Biol Chem. 2004;279:36259-36267.
41. Cabodi S, Calautti E, Talora C, et al. A PKC-eta/Fyn-dependent pathway leading to keratinocyte growth arrest and differentiation. Mol Cell. 2000;6:1121-1129.
42. Shields JM, Christy RJ, Yang VW. Identification and characterization of a gene encoding a gut-enriched Kruppel-like factor expressed during growth arrest. J Biol Chem. 1996;271:20009-20017.
43. Chen X, Whitney EM, Gao SY, et al. Transcriptional profiling of Kruppel-like factor 4 reveals a function in cell cycle regulation and epithelial differentiation. J Mol Biol. 2003;326:665-677.
44. Oni OO. The early stages of the repair of adult human diaphyseal fractures. Injury. 1997;28:521-525.
45. Santavirta S, Konttinen YT, Nordstrom D, et al. Immunologic studies of nonunited fractures. Acta Orthop Scand. 1992;63:579-586.
46. Banovac K, Renfree K, Makowski AL, et al. Fracture healing and mast cells. J Orthop Trauma. 1995;9:482-490.
47. Taniguchi H. Mast cells in fracture healing: An experimental study using rat model. Nippon Seikeigeka Gakkai Zasshi. 1990;64:949-957.
48. Romano SJ, Corriveau RA, Schwarz RI, et al. Expression of the nicotinic receptor alpha 7 gene in tendon and periosteum during early development. J Neurochem. 1997;68:640-648.
49. Meyer RA Jr, Desai BR, Meyer MH. Age alters mRNA gene expression in the fracture callus of rats by microarray analysis. Trans Orthop Res Soc. 2003;28:489. (Abstract)
50. Filimonov VI, Nedospasov VO, Stepanova NV. Erythropoiesis and osteogenesis (I. The interaction of the reparative processes of bone and hematopoietic tissues). Fiziol Zh. 1991;37:12-18.
51. Giuliani DC, Hall JC, Morse BS. Strategies of hemopoietic stress adaptation within the medullary cavity. Anat Rec. 1986;216:528-533.
52. Einhorn TA, Majeska RJ, Mohaideen A, et al. A single percutaneous injection of recombinant human bone morphogenetic protein-2 accelerates fracture repair. J Bone Joint Surg Am. 2003;85:1425-1435.
53. Komatsu DE, Hadjiargyrou M. Activation of the transcription factor HIF-1 and its target genes, VEGF, HO-1, iNOS, during fracture repair. Bone. 2004;34:680-688.
54. David SM, Gruber HE, Meyer RA Jr, et al. Lumbar spinal fusion using recombinant human bone morphogenetic protein in the canine. Spine. 1999;24:1973-1979.