Nucleofection of muscle-derived stem cells and myoblasts with φC31 integrase: Stable expression of a full-length-dystrophin fusion gene by human myoblasts

Molecular Therapy

Volumn 10, Issue 4, 2004, Pages 679-687

  Quenneville, Simon P ^a   Chapdelaine, Pierre ^a   Rousseau, Joél ^a   Beaulieu, Jean ^a   Caron, Nicolas J ^a   Skuk, Daniel ^a   Mills, Philippe ^a   Olivares, Eric C ^b   Calos, Michele P ^b   Tremblay, Jacques P ^a  

^a LAVAL UNIVERSITY   (Canada)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

C31 integrase; attB sequence; Duchenne muscular dystrophy (DMD); eGFP full length dystrophin; Ex vivo gene therapy; Nucleofection; Stem cells; Transplantation of nucleofected myogenic cells

Indexed keywords

DYSTROPHIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INTEGRASE; PLASMID DNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACTERIOPHAGE; CELL CULTURE; CELL TRANSPLANTATION; CONTROLLED STUDY; DNA INTEGRATION; DUCHENNE MUSCULAR DYSTROPHY; EX VIVO STUDY; FLUORESCENCE; FUSION GENE; GENE EXPRESSION; GENE THERAPY; GENETIC TRANSFECTION; GENOME; HUMAN; HUMAN CELL; MALE; MOUSE; MYOBLAST; MYOTUBE; NONHUMAN; NONVIRAL GENE THERAPY; PLASMID; PROTEIN EXPRESSION; STEM CELL; TRANSGENE;

ANIMALS; ARTIFICIAL GENE FUSION; ATTACHMENT SITES, MICROBIOLOGICAL; BACTERIOPHAGES; CELL LINE; CELL NUCLEUS; DYSTROPHIN; ELECTROPORATION; GENE THERAPY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; INTEGRASES; MICE; MUSCULAR DYSTROPHY, DUCHENNE; MYOBLASTS; PLASMIDS; TRANSFECTION;

EID: 4644292834     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymthe.2004.05.034     Document Type: Article

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