Nonlinear fitting of bisubstrate enzyme kinetic models using SAS computer software: Application to R67 dihydrofolate reductase

Analytical Biochemistry

Volumn 334, Issue 1, 2004, Pages 204-206

  Smiley, R D ^a   Saxton, A M ^a   Jackson, M J ^a   Hicks, S N ^a   Stinnett, L G ^a   Howell, E E ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

NONLINEAR FITTING; R67 DIHYDROFOLATE REDUCTASE;

APPLICATION PROGRAMS;

DIHYDROFOLATE REDUCTASE;

ARTICLE; BINDING AFFINITY; COMPUTER PROGRAM; ENZYME KINETICS; ENZYME SUBSTRATE; MICHAELIS CONSTANT; MOLECULAR MECHANICS; MOLECULAR MODEL; PRIORITY JOURNAL;

EID: 4644269136     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.06.040     Document Type: Article

References (9)

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3. SAS Institute, Inc, Cary, NC., SAS/STAT Software, Version 9.1, (2003) see http://www.sas.com/technologies/analytics/statistics/stat/
4. I.H. Segel Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady State Enzyme Systems 1975 Wiley New York
5. N. Narayana, D.A. Matthews, E.E. Howell, and N.H. Xuong A plasmid-encoded dihydrofolate reductase from trimethoprim-resistant bacteria has a novel D2-symmetric active site Nat. Struct. Biol. 2 1995 1018 1025
6. T.D. Bradrick, J.M. Beechem, and E.E. Howell Unusual binding stoichiometries and cooperativity are observed during binary and ternary complex formation in the single active pore of R67 dihydrofolate reductase, a D2 symmetric protein Biochemistry 35 1996 11414 11424
7. H. Park, T.D. Bradrick, and E.E. Howell A glutamine 67 → histidine mutation in homotetrameric R67 dihydrofolate reductase results in four mutations per single active site pore and causes substantial substrate and cofactor inhibition Protein Eng. 10 1997 1415 1424
8. M.B. Strader, S. Chopra, M. Jackson, R.D. Smiley, L.G. Stinnett, J. Wu, and E.E. Howell Defining the binding site of homotetrameric R67 dihydrofolate reductase and correlating binding enthalpy with catalysis Biochemistry 43 2004 7403 7412
9. R.D. Smiley, L.G. Stinnett, A.M. Saxton, and E.E. Howell Breaking symmetry: mutations engineered into R67 dihydrofolate reductase, a D2 symmetric homotetramer possessing a single active site pore Biochemistry 41 2002 15664 15675