Activation by Cdc42 and PIP2 of Wiskott-Aldrich Syndrome protein (WASp) stimulates actin nucleation by Arp2/3 complex

Journal of Cell Biology

Volumn 150, Issue 6, 2000, Pages 1311-1320

  Higgs, Henry N ^a   Pollard, Thomas D ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

GBD; Membrane; Neutrophil; Prenylation; Thymus

Indexed keywords

ACTIN; ARP 2 3 COMPLEX; CELL PROTEIN; CYCLIN DEPENDENT KINASE; GUANOSINE TRIPHOSPHATE; LIPID; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PROTEIN CDC42; RHO FACTOR; UNCLASSIFIED DRUG; WISKOTT ALDRICH SYNDROME PROTEIN;

ACTIN FILAMENT; ARTICLE; CATTLE; ENZYME ACTIVATION; MEMBRANE BINDING; NEUTROPHIL; NONHUMAN; PRENYLATION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; THYMUS;

ACTIN-RELATED PROTEIN 2; ACTIN-RELATED PROTEIN 3; ACTINS; ANIMALS; ANTIBODIES; CATTLE; CDC42 GTP-BINDING PROTEIN; CYTOSKELETAL PROTEINS; GTP-BINDING PROTEINS; HUMANS; LIPID METABOLISM; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MUSCLE, SKELETAL; NERVE TISSUE PROTEINS; NEUTROPHILS; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; PROTEIN ISOPRENYLATION; RABBITS; THYMUS GLAND; WISKOTT-ALDRICH SYNDROME; WISKOTT-ALDRICH SYNDROME PROTEIN, NEURONAL;

BOS TAURUS; BOVINAE; VESPIDAE;

EID: 0034683671     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.6.1311     Document Type: Article

References (53)

1. Structure of Cdc42 in complex with the GTPase-binding domain of the 'Wiskott-Aldrich Syndrome' protein
2. Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation
3. Fission yeast Sop2p: A novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin function
4. Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin
5. Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASp/Scar proteins
6. Motility of ActA protein-coated microspheres driven by actin polymerization
7. Grb2 links signalling to actin assembly by enhancing interaction of neural Wiskott-Aldrich Syndrome protein (N-WASP) with actin-related-protein (Arp2/3) complex
8. Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility
9. Structure of EVH1, a novel proline-rich ligand-binding module involved in cytoskeletal dynamics and neural function
10. Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling
11. Requirement for posttranslational processing of Rac GTP-binding proteins for activation of human neutrophil NADPH oxidase
12. Regulation of actin polymerization by Arp2/3 complex and WASp/Scar proteins
13. Influence of the Wiskott Aldrich syndrome protein (WASp) C terminus and Arp2/3 complex on actin polymerization
14. Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques
15. Autoinhibition and activation mechanisms of the Wiskott-Aldrich Syndrome protein
16. Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts
17. The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42
18. Scar1 and the related Wiskott-Aldrich syndrome protein WASP regulate the actin cytoskeleton through the Arp2/3 complex
19. Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin agarose
20. Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins
21. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex
22. Identification of a factor in conventional muscle actin preparation which inhibits actin filament self-association
23. Interaction of WASp/Scar proteins with actin and vertebrate Arp2/3 complex
24. The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin
25. Endocytic vesicles move at the tips of actin tails in cultured mast cells
26. 2-dependent manner downstream of tyrosine kinases
27. Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP
28. Cdc42 is required for membrane dependent actin polymerization in vitro
29. A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast
30. Rho-family GTPases require the Arp2/3 complex to stimulate actin polymerization in Acanthamoeba extracts
31. The interaction of Arp2/3 complex with actin: Nucleation, high-affinity pointed end capping, formation of branching networks of filaments
32. Rho, rac, cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, filopodia
33. Kinetics of Cdc42 membrane extraction by Rho-GDI monitored by real-time fluorescence resonance energy transfer
34. The Wiskott-Aldrich Syndrome
35. Quantitative analysis of the effect of Acanthamoeba profilin on actin filament nucleation and elongation
36. Structure of the Enabled/VASP Homology 1 domain-peptide complex: A key component in the spatial control of actin assembly
37. The small GTP-binding proteins in the cytosol of insulin-secreting cells are complexed to GDP dissociation inhibitor proteins
38. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
39. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin
40. Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex
41. Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia
42. Actin-dependent propulsion of endosomes and lysosomes by recruitment of N-WASP
43. Volume and osmotic properties of human neutrophils
44. Interactions of Acanthamoeba profilin with actin and nucleotides bound to actin
45. Spatial control of actin polymerization during neutrophil chemotaxis
46. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly
47. Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes
48. Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation
49. Activation of the yeast Arp2/3 complex by Bee1p, a WASP-family protein
50. Genetic dissection of the budding yeast Arp2/3 complex: A comparison of the in vivo and structural roles of individual subunits
51. The Wiskott-Aldrich syndrome protein directs actin-based motility by stimulating actin nucleation with the Arp2/3 complex
52. Wiskott-Aldrich syndrome/X-linked thrombocytopenia: WASp gene mutations, protein expression, phenotype
53. Regulation of actin polymerization in cell-free systems by GTPγS and Cdc42