Crystal structures of unbound and aminooxyacetate-bound Escherichia coli γ-aminobutyrate aminotransferase

Biochemistry

Volumn 43, Issue 34, 2004, Pages 10896-10905

  Liu, Wenshe ^a   Peterson, Peter E ^a   Carter, Richard J ^a   Zhou, Xianzhi ^a   Langston, James A ^a   Fisher, Andrew J ^a   Toney, Michael D ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CARBOXYLATION; CRYSTAL STRUCTURE; CRYSTALLIZATION; ESCHERICHIA COLI; REACTION KINETICS; X RAY ANALYSIS;

AMINOTRANSFERASE; BINDING SITES; HOMOLOGOUS ENZYMES; IRON-SULFUR CLUSTERS;

BIOCHEMISTRY;

4 AMINOBUTYRATE AMINOTRANSFERASE; 4 AMINOBUTYRIC ACID; ALPHA AMINO ACID; AMINOOXYACETIC ACID; ARGININE; BACTERIAL ENZYME; CARBOXYL GROUP; CARBOXYLYASE; GLUTAMIC ACID; IRON SULFUR PROTEIN; TETRAMER; 2,2 DIALKYLGLYCINE DECARBOXYLASE; 2,2-DIALKYLGLYCINE DECARBOXYLASE; ENZYME INHIBITOR; ESCHERICHIA COLI PROTEIN; LIGAND; PYRIDOXAL 5 PHOSPHATE; VIGABATRIN;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SWINE; X RAY DIFFRACTION; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; DRUG ANTAGONISM; METABOLISM; PROTEIN BINDING; STRUCTURAL HOMOLOGY; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SUS SCROFA;

4-AMINOBUTYRATE TRANSAMINASE; AMINOOXYACETIC ACID; ANIMALS; BINDING SITES; CARBOXY-LYASES; CRYSTALLOGRAPHY, X-RAY; ENZYME INHIBITORS; ESCHERICHIA COLI PROTEINS; LIGANDS; MODELS, MOLECULAR; PROTEIN BINDING; PYRIDOXAL PHOSPHATE; STRUCTURAL HOMOLOGY, PROTEIN; SUBSTRATE SPECIFICITY; SWINE; VIGABATRIN;

EID: 4644248835     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049218e     Document Type: Article

References (33)

1. Schwartz, J. H. (1991) Principles of Neural Science, Prentice Hall International, Norwalk.
2. Lloyd, K. G. (1986) GABAergic theory of epilepsy, Rev. Prat. 36, 243-54.
3. Lloyd, K. G., Morselli, P. L., and Bartholini, G. (1987) GABA and affective disorders, Med. Biol. 65, 159-65.
4. Wong, C. G., Bottiglieri, T., and Snead, O. C., 3rd. (2003) GABA, gamma-hydroxybutyric acid, and neurological disease, Ann. Neurol. 54 Suppl. 6, S3-12.
5. Sarup, A., Larsson, O. M., and Schousboe, A. (2003) GABA transporters and GABA-transaminase as drug targets Curr Drug Target, CNS Neurol. Disord. 2, 269-77.
6. Mehta, P. K., Hale, T. I., and Christen, P. (1993) Aminotransferases: demonstration of homology and division into evolutionary subgroups, Eur. J. Biochem. 214, 549-61.
7. Iadarola, M. J., and Gale, K. (1981) Cellular compartments of GABA in brain and their relationship to anticonvulsant activity, Mol. Cell Biochem. 39, 305-29.
8. Sherif, F. M. (1994) GABA-transaminase in brain and blood platelets: basic and clinical aspects, Prog. Neuropsychopharmacol. Biol. Psychiatry 18, 1219-33.
9. Sherif, F. M., and Ahmed, S. S. (1995) Basic aspects of GABA-transaminase in neuropsychiatric disorders, Clin. Biochem. 28, 145-54.
10. Sherif, F. M., Tawati, A. M., Ahmed, S. S., and Sharif, S. I. (1997) Basic aspects of GABA-transmission in alcoholism, with particular reference to GABA-transaminase, Eur. Neuropsychopharmacol. 7, 1-7.
11. Wallach, D. P. (1961) Studies on the GABA pathway. I. The inhibition of gamma-aminobutyric acid-alpha-ketoglutaric acid transaminase in vitro and in vivo by U-7524 (amino-oxyacetic acid), Biochem. Pharmacol. J, 323-31.
12. Loscher, W., and Frey, H. H. (1978) Aminooxyacetic acid: correlation between biochemical effects, anticonvulsant action and toxicity in mice, Biochem. Pharmacol. 27, 103-8.
13. Kuriyama, K., Roberts, E., and Rubinstein, M. K. (1966) Elevation of gamma-aminobutyric acid in brain with amino-oxyacetic acid and susceptibility to convulsive seizures in mice: a quantitative reevaluation, Biochem. Pharmacol. 15, 221-36.
14. Bartsch, K., von Johnn-Marteville, A., and Schulz, A. (1990) Molecular analysis of two genes of the Escherichia coli gab cluster: Nucleotide sequence of the glutamate: succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD), J. Bacteriol. 172, 7035-42.
15. Bruker (1998), Bruker AXS Inc., Madison, Wisconsin, United States.
16. Collaborative Computational Project, N. (1994) The CCP4 suite: programs for protein crystallography, Acta Crystallogr. D50, 760-763.
17. Navaza, J. (2001) Implementation of molecular replacement in AMoRe Acta Crystallogr., Sect. D 57, 1367-72.
18. Cowtan, K. D., and Zhang, K. Y. (1999) Density modification for macromolecular phase improvement, Prog. Biophys. Mol. Biol. 72, 245-70.
19. Brunger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Crystallography & NMR system: A new software suite for macromolecular structure determination, Acta Crystallogr., Sect. D 54 (Pt 5), 905-21.
20. Storici, P., De Biase, D., Bossa, F., Bruno, S., Mozzarelli, A., Peneff, C., Silverman, R. B., and Schirmer, T. (2004) Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5′-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin, J. Biol. Chem. 279, 363-73.
21. Storici, P., Capitani, G., De Biase, D., Moser, M., John, R. A., Jansonius, J. N., and Schirmer, T. (1999) Crystal structure of GABA-aminotransferase, a target for antiepileptic drug therapy, Biochemistry 38, 8628-34.
22. Toney, M. D., Hohenester, E., Keller, J. W., and Jansonius, J. N. (1995) Structural and mechanistic analysis of two refined crystal structures of the pyridoxal phosphate-dependent enzyme dialkylglycine decarboxylase, J. Mol. Biol. 245, 151-79.
23. Shen, B. W., Hennig, M., Hohenester, E., Jansonius, J. N., and Schirmer, T. (1998) Crystal structure of human recombinant ornithine aminotransferase, J. Mol. Biol. 277, 81-102.
24. Hennig, M., Grimm, B., Contestabile, R., John, R. A., and Jansonius, J. N. (1997) Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity, Proc. Natl. Acad. Sci. U.S.A. 94, 4866-71.
25. Malashkevich, V. N., Strop, P., Keller, J. W., Jansonius, J. N., and Toney, M. D. (1999) Crystal structures of dialkylglycine decarboxylase inhibitor complexes, J. Mol. Biol. 294, 193-200.
26. Liu, W., Rogers, C. J., Fisher, A. J., and Toney, M. D. (2002) Aminophosphonate inhibitors of dialkylglycine decarboxylase: structural basis for slow binding inhibition, Biochemistry 41, 12320-8.
27. Shah, S. A., Shen, B. W., and Brunger, A. T. (1997) Human ornithine aminotransferase complexed with L-canaline and gabaculine: structural basis for substrate recognition, Structure 5. 1067-75.
28. Storici, P., Capitani, G., Muller, R., Schirmer, T., and Jansonius, J. N. (1999) Crystal structure of human ornithine aminotransferase complexed with the highly specific and potent inhibitor 5-fluoromethylornithine, J. Mol. Biol. 285, 297-309.
29. John, R. A. (1995) Pyridoxal phosphate-dependent enzymes, Biochem. Biophys. Acta 1248, 81-96.
30. Toney, M. D., and Kirsch, J. F. (1993) Lysine 258 in aspartate aminotransferase: enforcer of the Circe effect for amino acid substrates and general-base catalyst for the 1,3-prototropic shift, Biochemistry 32, 1471-9.
31. John, R. A., and Charteris, A. (1978) The reaction of aminooxyacetate with pyridoxal phosphate-dependent enzymes, Biochem. J. 171, 771-9.
32. Sun, S., Zabinski, R. F., and Toney, M. D. (1998) Reactions of alternate substrates demonstrate stereoelectronic control of reactivity in dialkylglycine decarboxylase, Biochemistry 37, 3865-75.
33. Sun, S., Bagdassarian, C. K., and Toney, M. D. (1998) Pre-steady-state kinetic analysis of the reactions of alternate substrates with dialkylglycine decarboxylase, Biochemistry 37, 3876-85.