The rdxA gene plays a more major role than frxA gene mutation in high-level metronidazole resistance of Helicobacter pylori in Taiwan

Helicobacter

Volumn 9, Issue 5, 2004, Pages 400-407

  Yang, Yao Jong ^a,b   Wu, Jiunn Jong ^b   Sheu, Bor Shyang ^a,b   Kao, Ai Wen ^b   Huang, Ay Huey ^b  

^a NATIONAL CHENG KUNG UNIVERSITY HOSPITAL   (Taiwan)

^b NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

Antibiotic resistance; Gene mutation; Nitroreductase

Indexed keywords

METRONIDAZOLE;

ANTIBIOTIC RESISTANCE; ARTICLE; FRXA GENE; GENE; GENE MUTATION; GENE SEQUENCE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RDXA GENE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; STOMACH BIOPSY; TAIWAN;

AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; BACTERIAL PROTEINS; BIOPSY; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; HELICOBACTER PYLORI; HUMANS; METRONIDAZOLE; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MUTATION; NITROREDUCTASES; OXIDOREDUCTASES; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ALIGNMENT; TAIWAN;

HELICOBACTER; HELICOBACTER PYLORI;

EID: 4644221245     PISSN: 10834389     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1083-4389.2004.00270.x     Document Type: Article

References (22)

1. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984;1:1311-5.
2. Uemura N, Okamoto S, Yamamoto S, et al. Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 2001;345:784-9.
3. Forbes GM, Glaser ME, Cullen DJ, et al. Duodenal ulcer treated with Helicobacter pylori eradication: seven-year follow-up. Lancet 1994;343:258-60.
4. Huang GC, Sheu BS, Tsao CJ, Lin XZ, Su IJ. Eradiation of H. pylori results in regression of B-cell low grade gastric MALToma with evident B-symptoms. Hepatogastroenterology 1998;45:2464-7.
5. Bazzoli F, Zagari RM, Pozzato P, et al. Low-dose lansoprazole and clarithromycin plus metronidazole vs. full-dose lansoprazole and clarithromycin plus amoxicillin for eradication of Helicobacter pylori infection. Aliment Pharmacol Ther 2002; 16:153-8.
6. Dore MP, Leandro G, Realdi G, Sepulveda AR, Graham DY. Effect of pretreatment antibiotic resistance to metronidazole and clarithromycin on outcome of Helicobacter pylori therapy: a meta-analytical approach. Dig Dis Sci 2000;45:68-76.
7. Huang AH, Sheu BS, Yang HB, Huang CC, Wu JJ, Lin XZ. Impact of Helicobacter pylori antimicrobial resistance on the outcome of 1-week lansoprazole-based triple therapy. J Formos Med Assoc 2000;99:704-9.
8. Kim JJ, Reddy R, Lee M, et al. Analysis of metronidazole, clarithromycin and tetracycline resistance of Helicobacter pylori isolates from Korea. J Antimicrob Chemother 2001;47:459-61.
9. Meyer JM, Silliman NP, Wang W, et al. Risk factors for Helicobacter pylori resistance in the United States: the surveillance of H. pylori antimicrobial resistance partnership (SHARP) study, 1993-99. Ann Intern Med 2002;136:13-24.
10. Goodwin A, Kersulyte D, Sisson G, Veldhuyzen Van Zanten SJO, Berg DE, Hoffman PS. Metronidazole resistance in Helicobacter pylori is due to null mutations in a gene (rdxA) that encodes an oxygen-insensitive NADPH nitroreductase. Mol Microbiol 1998;28:383-93.
11. Kwon DH, El-Zaatari FAK, Kato M, et al. Analysis of rdxA and involvement of additional genes encoding NAD (P) H flavin oxidoreductase (FrxA) and ferredoxin-like protein (FdxB) in metronidazole resistance of Helicobacter pylori. Antimicrob Agents Chemother 2000;44:2133-42.
12. Kwon DH, Hulten K, Kato M, et al. DNA sequence analysis of rdxA and frxA from 12 pairs of metronidazole-sensitive and - resistant clinical Helicobacter pylori isolates. Antimicrob Agents Chemother 2001;45:2609-15.
13. Jeong JY, Mukhopadhyay AK, Dailidiene D, et al. Sequential inactivation of rdxA (HP0954) and frxA (HP0642) nitroreductase genes causes moderate and high-level metronidazole resistance in Helicobacter pylori. J Bacteriol 2000;182:5082-90.
14. Kato S, Fujimura S, Udagawa H, et al. Antibiotic resistance of Helicobacter pylori strains in Japanese children. J Clin Microbiol 2002;40:649-53.
15. Arents NLA, Smeets LC, van Zwet AA, et al. Implications of the simultaneous presence of metronidazole-susceptible and - resistant Helicobacter pylori colonies within a single biopsy specimen. Eur J Clin Microbiol Infect Dis 2001;20:418-20.
16. Wikler MA, Cockerill FR, Craig WA, et al. National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Growth Aerobically-Twelfth Informational (Suppl.): Approved standard M7-A5. NCCLS, Villanova, PA, USA, 2002.
17. Fujimoto S, Marshall B, Blaser MJ. PCR-based restriction fragment length polymorphism typing of Helicobacter pylori. J Clin Microbiol 1994;32:331-4.
18. Kwon DH, Kato M, El-Zaatari FAK, Osato MS, Graham DY. Frame-shift mutations in NAD (P) H flavin oxidoreductase encoding gene (frxA) from metronidazole resistant Helicobacter pylori ATCC43504 and its involvement in metronidazole resistance. FEMS Microbiol Lett 2000;188:197-202.
19. Marais A, Bilardi C, Cantet F, Mendz GL, Megraud F. Characterization of the genes rdxA and frxA involved in metronidazole resistance in Helicobacter pylori. Res Microbiol 2003;154:137-44.
20. Weel JF, van der Hulst RW, Gerrits Y, Tytgat GN, van der Ende A, Dankert J. Heterogeniety in susceptibility to metronidazole among Helicobacter pylori isolates from patients with gastritis or peptic ulcer disease. J Clin Microbiol 1996;34:2158-62.
21. Bereswill S, Krainick C, Stähler F, Herrmann L, Kist M. Analysis of the rdxA gene in high-level metronidazole-resistant clinical isolates confirms a limited use of rdxA mutations as a marker for prediction of metronidazole resistance in Helicobacter pylori. FEMS Immunol Med Microbiol 2003;36:193-8.
22. Tankovic J, Lamarque D, Delchier JC, Soussy CJ, Labigne A, Jenks PJ. Frequent association between alteration of the rdxA gene and metronidazole resistance in French and North African isolates of Helicobacter pylori. Antimicrob Agents Chemother 2000;44:608-13.