A simple one-step real-time RT-PCR for diagnosis of dengue virus infection

Journal of Medical Virology

Volumn 80, Issue 8, 2008, Pages 1426-1433

  Dos Santos, Harryson Wings Godoy ^a   Poloni, Telma Regina Ramos Silva ^a   Souza, Kelly Paula ^a   Muller, Vanessa Danielle Menjon ^a   Tremeschin, Flávia ^a   Nali, Lívia Christensen ^a   Fantinatti, Leandro Ricardo ^a   Amarilla, Alberto Anastacio ^a   Castro, Helda Liz Alfonso ^a   Nunes, Marcio Roberto ^b   Casseb, Samir Mansour ^b   Vasconcelos, Pedro Fernando ^b   Badra, Soraya Jabur ^a   Figueiredo, Luiz Tadeu Moraes ^a   Aquino, Victor Hugo ^a,c  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b INSTITUTO EVANDRO CHAGAS   (Brazil)

^c NONE   (Brazil)

Author keywords

Dengue; Diagnosis; Real time RT PCR

Indexed keywords

ARTICLE; BLOOD SAMPLING; DENGUE; DENGUE VIRUS; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS GENOME;

ADOLESCENT; ADULT; AGED; CHILD; DENGUE; DENGUE HEMORRHAGIC FEVER; DENGUE VIRUS; FEMALE; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; MALE; MIDDLE AGED; ORGANIC CHEMICALS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

ARBOVIRUS; DENGUE VIRUS;

EID: 46049088687     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.21203     Document Type: Article

References (40)

1. Alcon A, Talarmin M, Debruyne A, Falconar V, Deubel M. 2002. Flamand, enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40:376-381.
2. Aquino VH, Anatriello E, Gonçalves PF, Silva EV, Vasconcelos PFC, Vieira DS, Batista WC, Bobadilla ML, Vazquez C, Moran M, Figueiredo LTM. 2006. Molecular epidemiology of dengue type 3 virus in Brazil and Paraguay, 2002-2004. Am J Trop Med Hyg 75:710-715.
3. Bronzoni RVM, Baleotti FG, Nogueira RMR, Nunes M, Figueiredo LTM. 2005. Duplex reverse transcription-PCR followed by nested PCR assays for detection and identification of Brazilian alpha-viruses and flaviviruses. J Clin Microbiol 43:696-702.
4. Callahan JD, Wu SL, Dion-Schultz A, Mangold BE, Peruski LF, Watts DM, Porter KR, Murphy GR, Suharyona W, King CC, Hayes CR, Temeenak JJ. 2001. Development and evaluation of serotype- and group-specific fluorogenic reverse-transcriptase PCR (TaqMan) assays for dengue virus. J Clin Microbiol 39:4119-4124.
5. Chao DY, Davis BS, Chang GJ. 2007. Development of multiplex real-time reverse transcriptase PCR assays for detecting eight medically important flaviviruses in mosquitoes. J Clin Microbiol 45:584-589.
6. Chutinimitkul S, Payungporn S, Theamboonlers A, Poovorawan Y. 2005. Dengue typing assay based on real-time PCR using SYBR Green I. J Virol Methods 129:8-15.
7. Deubel V, Laille M, Hugnot JP, Chungue E, Guesdom JL, Drouet MT, Bassot S, Chevrier D. 1990. Identification of dengue sequences by genomic amplification: Rapid diagnosis of dengue virus serotypes in peripheral blood. J Virol Methods 30:41-54.
8. Deubel V, Huerre M, Cathomas G, Drouet MT, Wuscher N, LeGuenno B, Widmer AF. 1997. Molecular detection and characterization of yellow fever virus in blood and liver specimens of a non-vaccinated fatal human case. J Med Virol 53:212-217.
9. Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, Gunther S. 2002. Rapid detection and quantitation of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol 40:2323-2330.
10. Dyer J, Chisenhall DM, Mores CN. 2007. A multiplexed TaqMan assay for the detection of arthropod-borne flaviviruses. J Virol Methods 145:9-13.
11. Figueiredo LTM, Owa A, Carlucci RH, de Oliveira L. 1992. Laboratory diagnosis and symptoms of dengue, during an outbreak in the Ribeirao Preto region, SP, Brazil. Rev Inst Med Trop Sao Paulo March-April 34:121-130.
12. Figueiredo LTM, Batista WC, Igarashi A. 1997a. A simple reverse transcription-polymerase chain reaction for dengue type 2 virus identification. Membr Inst Oswaldo Cruz 92:395-398.
13. Figueiredo LTM, Batista WC, Igarashi A. 1997b. Detection and identification of dengue virus isolates from Brazil by a simplified reverse transcription-polymerase chain reaction (RT-PCR) method. Rev Inst Med Trop (São Paulo) 39:95-99.
14. Gibbons RV, Vaughn DW. 2002. Dengue: An escalating problem. Br Med J 324:1563-1566.
15. Gubler DJ. 2002. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol 10:100-103.
16. Gubler DJ, Kuno G, Sather GE, Velez M, Oliver A. 1984. Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses. Am J Trop Med Hyg 33:158-165.
17. Guzman MG, Kouri G. 2002. Dengue: An update. Lancet Infect Dis 2:33-42.
18. Halstead SB. 1988. Pathogenesis of dengue: Challenges to molecular biology. Science 239:476-481.
19. Henchal EA, Putnak JR. 1990. The dengue viruses. Clin Microbiol Rev 3:376-396.
20. Houng HH, Chen RCM, Vaughn DW, Kanesa-Thesan N. 2001. Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes1-4 using conserved and serotype-specific 3′ noncoding sequences. J Virol Methods 95:19-32.
21. Johnson BW, Russell BJ, Lanciotti RS. 2005. Serotype-specific detection of dengue viruses in a four plex real-time reverse transcriptase PCR assay. J Clin Microbiol 43:4977-4983.
22. Kong YY, Thay CH, Tin TC, Devi. S. 2006. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. J Virol Methods 138:123-130.
23. Kumarasamy V, Chua SK, Hassan Z, Wahab AH, Chem YK, Mohamad M, Chua KB. 2007a. Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection. Singapore Med J 48:669-673.
24. Kumarasamy V, Wahab AH, Chua SK, Hassan Z, Chem YK, Mohamad M, Chua KB. 2007b. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J Virol Methods 140:75-79.
25. Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE, Ng. LC. 2007. Cost effective real-time reverse transcriptase PCR (RT-PCR) to screen for Dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J Clin Microbiol 45:935-941.
26. Lanciotti RS, Calisher CH, Gubler DJ, Chang GL, Vorndam V. 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30:545-551.
27. Laue T, Emmerich P, Schmitz H. 1999. Detection of dengue virus RNA in patients after primary or secondary dengue infection by using the TaqMan automated amplification system. J Clin Microbiol 37:2543-2547.
28. Libraty DH, Endy TP, Houng HSH, Green S, Kalayanaroqj S, Suntayakorn S, Chansiriwongs W, Vaughn DW, Nisalak A, Ennis FA, Rothman AL. 2002. Differing influence of virus burden and immune activation on disease severity in secondary dengue-3 virus infection. J Infect Dis 185:1213-1221.
29. Monath TP. 1994. Dengue, the risk to developed and developing countries. Proc Natl Acad Sci U S A 91:2395-2400.
30. Monath TP, Heinz FX. 1996. Flaviviruses. In: Fields BW, Knipe DM, Knipe PM, Howley PM, editors. Fields Virology Vol. 1 3rd ed. Philadelphia: Lippincott-Raven Press. 961-1034.
31. Murgue B, Roche C, Chungue E, Deparis X. 2000. Prospective study of the duration of magnitude of viremia in children hospitalized during the 1996-1997 dengue-2 outbreak in French Polynesia. J Med Virol 60:432-438.
32. Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Lin TH, Huang JH. 2003a. Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections. Clin Diagn Lab Immunol 10:622-630.
33. Shu PY, Chang SF, Kuo YC, Yueh YY, Chien LJ, Sue CL, Lin TH, Huang JH. 2003b. Development of group- and serotype-specific one-step SYBR Green I-based real-time reverse transcription-PCR assay for dengue virus. J Clin Microbiol 41:2408-2416.
34. Vaughn DW, Green S, Kalayanaroqj S, Innis BL, Nimmannitya S, Suntayakorn S, Endy TP, Raengsakulrach B, Rothman AL, Ennis FA, Nisalak A. 2000. Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity. J Infect Dis 181:2-9.
35. Vorndam AV, Kuno G. 1997. Laboratory diagnosis of dengue virus infections. In: Gubler DJ, Kuno G, editors. Dengue and Dengue Hemorrhagic Fever. New York: CAB International. 313-333.
36. Wang WK, Chao DY, Kao CL, Wu HC, Liu YC, Li CM, Lin SC, Ho ST, Huang JH, Kingb CC. 2003. High levels of plasma dengue viral load during defervescence in patients with dengue hemorrhagic fever: Implications for pathogenesis. Virology 305:330-338.
37. Warrilow D, Northill JA, Pyke A, Smith GA. 2002. Single rapid TaqMan fluorogenic probe-based PCR assay that detects all four dengue serotypes. J Med Virol 66:524-528.
38. World Health Organization. 1986. Technical Advisory Committee on dengue haemorrhagic fever for the Southeast Asian and Western Pacific regions. Guide for diagnosis, treatment and control of dengue haemorrhagic fever, Geneva.
39. World Health Organization (WHO). 1997. Dengue hemorrhagic fever, diagnosis, treatment and control. 2nd ed. Geneva: WHO, 1997.
40. Yong YK, Thayan R, Chong HT, Tan CT, Sekaran SD. 2007. Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR. Singapore Med J 48:662-668.