Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR

Singapore Medical Journal

Volumn 48, Issue 7, 2007, Pages 662-668

  Yong, Y K ^a   Thayan, R ^a   Chong, H T ^a   Tan, C T ^a   Sekaran, S D ^a  

^a UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

Conventional multiplex RT PCR; Dengue virus; Melt curve analysis; Real time SYBR Green RT PCR

Indexed keywords

IMMUNOGLOBULIN M; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; DENGUE VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PUBLIC HEALTH SERVICE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; BLOOD; DENGUE; EVALUATION; GENETICS; HUMAN; METHODOLOGY; MOLECULAR GENETICS;

DENGUE; DENGUE VIRUS; HUMANS; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

EID: 34547411895     PISSN: 00375675     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Gubler DJ. Dengue and dengue hemorrhagic fever: its history and resurgence as a global public health problem. In: Gubler DJ, Kuno G, eds. Dengue and Dengue Hemorrhagic Fever. New York: CAB International, 1997: 1-22.
2. Halstead SB, O'Rourke EJ, Allison AC. Dengue viruses and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supporting in vitro infection. J Exp Med 1977; 146:218-29.
3. Deubel V, Kinney RM, Trent DW. Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype: comparative analysis of the full-length genome. Virology 1988; 165:234-44.
4. Rigau-Pérez JG, Gubler DJ, Vorndam AV, Clark GG. Dengue surveillance - United States 1986-1992. MMWR CDC Surveill Summ 1994; 43:7-19.
5. Gubler DJ. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 1998; 11:480-96.
6. Halstead SB. Pathogenesis of dengue: challenges to molecular biology. Science 1988; 239:476-81.
7. Monath TP. Dengue, the risk to developed and developing countries. Proc Natl Acad Sci U S A 1994; 91:2395-400.
8. Innis BL. Dengue and dengue hemorrhagic fever. In: Porterfield JS, ed. Kass Handbook of Infectious Diseases: Exotic Viral Infections. London: Chapman & Hall Medical, 1995: 103-46.
9. Harris E, Roberts TG, Smith L, et al. Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCR. J Clin Microbiol 1998; 36:2634-9.
10. Alcon S, Talarmin A, Debruyne M, et al. Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 2002; 40:376-81.
11. World Health Organization. Dengue hemorrhagic fever: diagnosis, treatment, prevention and control. 2nd ed. Geneva: World Health Organization, 1997.
12. Vorndam AV, Kuno G. Laboratory diagnosis of dengue virus infections. In: Gubler DJ, Kuno G, eds. Dengue and Dengue Hemorrhagic Fever. New York: CAB International, 1997: 313-33.
13. Henchal EA, Gentry MK, McCown JM, Brandt WE. Dengue virusspecific and flavivirus group determinants idendified with monoclonal antibodies by indirect immunofluorescence. Am J Trop Med Hyg 1982; 31:830-6.
14. Gubler DJ, Kuno G, Sather GE, Velez M, Oliver A. Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses. Am J Trop Med Hyg 1984; 33:158-65.
15. Rossi CA, Drabick JJ, Gambel JM, et al. Laboratory diagnosis of acute dengue fever during the United Nations Mission in Haiti, 1995-1996. Am J Trop Med Hyg 1998; 59:275-8.
16. Morita K, Tanaka M, Igarashi A. Rapid identification of dengue virus serotypes by using polymerase chain reaction. J Clin Microbiol 1991; 29:2107-10.
17. Laue T, Emmerich P, Schmitz H. Detection of dengue virus RNA in patients after primary or secondary dengue infection using the TaqMan automated amplification system. J Clin Microbiol 1999; 2543-7.
18. Chang GJ, Trent DW, Vorndam AV, et al. An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses. J Clin Microbiol 1994; 32:477-83.
19. Chow VT. Molecular diagnosis and epidemiology of dengue virus infection. Ann Acad Med Singapore 1997; 26:820-6.
20. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 1992; 30:545-51.
21. Yong YK, Chong HT, Tan CT, Sekaran SD. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RTPCR. J Virol Methods 2006; 138:123-30.