Microanalysis of an antimicrobial peptide, β-defensin-2, in the stratum corneum from patients with atopic dermatitis

British Journal of Dermatology

Volumn 159, Issue 1, 2008, Pages 97-104

  Asano, S ^a   Ichikawa, Y ^b   Kumagai, T ^a   Kawashima, M ^a   Imokawa, G ^a,c  

^a TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

^b KAO CORPORATION   (Japan)

^c TOKYO UNIVERSITY OF TECHNOLOGY   (Japan)

Author keywords

defensin 2; Antimicrobial peptide; Atopic dermatitis; Staphylococcus aureus colonization

Indexed keywords

BETA DEFENSIN 2; PEPTIDE;

ADULT; ARTICLE; ATOPIC DERMATITIS; BACTERIUM COLONY; CONTROLLED STUDY; CORRELATION ANALYSIS; DISEASE SEVERITY; HUMAN; HUMAN TISSUE; IMMUNOPRECIPITATION; MAJOR CLINICAL STUDY; MICROANALYSIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEOMICS; STAPHYLOCOCCUS AUREUS; STRATUM CORNEUM; TISSUE LEVEL; WESTERN BLOTTING;

ADOLESCENT; ADULT; BETA-DEFENSINS; BLOTTING, WESTERN; DERMATITIS, ATOPIC; DOWN-REGULATION; EPIDERMIS; FEMALE; HUMANS; IMMUNOPRECIPITATION; MALE; MIDDLE AGED; STAPHYLOCOCCUS AUREUS; UP-REGULATION;

EID: 45749134172     PISSN: 00070963     EISSN: 13652133     Source Type: Journal    
DOI: 10.1111/j.1365-2133.2008.08613.x     Document Type: Article

References (19)

1. Akiyama H, Ueda M, Toi Y et al. Comparison of the severity of atopic dermatitis lesions and the density of Staphylococcus aureus on the lesions after antistaphylococcal treatment. J Infect Chemother 1996 2 : 70 4.
2. Imayama S, Shiimozono Y, Hoashi M et al. Reduced secretion of IgA to skin surface of patients with atopic dermatitis. J Allergy Clin Immunol 1994 94 : 165 200.
3. Imokawa G, Abe A, Jin K et al. Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin? J Invest Dermatol 1991 96 : 523 6.
4. Arikawa J, Ishibashi M, Kawashima M et al. Decreased levels of sphingosine, a natural anti-microbial agent, may be associated with vulnerability of the stratum corneum from patients with atopic dermatitis to colonization by Staphylococcus aureus. J Invest Dermatol 2002 119 : 433 9.
5. Huh WK, Oono T, Shirafuji Y et al. Dynamic alteration of human beta-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin. J Mol Med 2002 80 : 678 84.
6. Ong PY, Ohtake T, Brandt C et al. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N Engl J Med 2002 347 : 1151 60.
7. Ali RS, Falconer A, Ikram M et al. Expression of the peptide antibiotics human beta defensin-1 and human beta defensin-2 in normal human skin. J Invest Dermatol 2001 117 : 106 11.
8. Oren A, Ganz T, Liu L et al. In human epidermis, beta-defensin 2 is packed in lamellar bodies. Invest Ophthalmol Vis Sci 2003 44 : 1859 65.
9. Hanifin JM, Rajka G. Diagnostic features of atopic dermatitis. Acta Derm Venereol (Stockh) 1980 92 (Suppl. 44 7.
10. Rajka G, Langeland T. Grading of the severity of atopic dermatitis. Acta Derm Venereol (Stockh) 1989 144 (Suppl. 13 14.
11. Yang D, Chetrov O, Bykovskaia SN et al. Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science 1999 286 : 525 8.
12. Huttner KM, Bevins CL. Antimicrobial peptides as mediators of epithelial host defense. Pediatr Res 1999 45 : 785 94.
13. Frohm Nilsson M, Sandstedt B, Sørensen O et al. The human cationic antimicrobial protein (hCAP18), a peptide antibiotic, is widely expressed in human squamous epithelia and colocalizes with interleukin-6. Infect Immun 1999 67 : 2561 6.
14. Frohm M, Agerbath B, Ahangari G et al. The expression of the gene encoding for the antibacterial peptide LL-37 is induced in human keratinocytes during inflammatory disorders. J Biol Chem 1997 272 : 15258 63.
15. Harder J, Bartels J, Christophers E et al. A peptide antibiotic from human skin. Nature 1997 387 : 861 (Letter).
16. Dinulos JG, Mentele L, Fredericks LP et al. Keratinocyte expression of human beta-defensin 2 following bacterial infection: role in cutaneous host defense. Clin Diagn Lab Immunol 2003 10 : 161 6.
17. Lui AY, Destoumieux D, Wong AV et al. Human beta-defensin-2 production in keratinocytes is regulated by interleukin-1, bacteria, and the state of differentiation. J Invest Dermatol 2002 118 : 275 81.
18. Niyonsaba F, Ushio H, Nagaoka I et al. The human beta-defensins (-1, -2, -3, -4) and cathelicidin LL-37 induce IL-18 secretion through p38 and ERK MAPK activation in primary human keratinocytes. J Immunol 2005 175 : 1776 84.
19. Terai K, Sano Y, Kawasaki S et al. Effects of dexamethasone and cyclosporin A on human beta-defensin in corneal epithelial cells. Exp Eye Res 2004 79 : 175 80.