Cell sorting analysis of cell cycle-dependent X-ray sensitivity in end joining-deficient human cells

Biochemical and Biophysical Research Communications

Volumn 372, Issue 4, 2008, Pages 662-667

  Iwabuchi, Kuniyoshi ^a   Hashimoto, Mitsumasa ^a   Matsui, Tadashi ^a   Kurosawa, Aya ^b   Adachi, Noritaka ^b   Date, Takayasu ^a  

^a KANAZAWA MEDICAL UNIVERSITY   (Japan)

^b YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Cell cycle; Cell sorter; DNA double strand breaks; DT40; Flow cytometry; Nalm 6; Nonhomologous end joining; X ray sensitivity

Indexed keywords

CHEMICAL AGENT; DOUBLE STRANDED DNA; FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; CELL POPULATION; CELL SELECTION; COLONY FORMING CELL; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; DOUBLE STRANDED DNA BREAK; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IONIZING RADIATION; NONHUMAN; PRIORITY JOURNAL; RADIOSENSITIVITY; STANDARD;

CELL PROLIFERATION; CELL SEPARATION; DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; DNA REPAIR; FLOW CYTOMETRY; G1 PHASE; HELA CELLS; HISTONES; HUMANS; RADIATION TOLERANCE; RECOMBINATION, GENETIC; X-RAYS;

EID: 45449090406     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.05.147     Document Type: Article

References (18)

1. Jackson S.P. Sensing and repairing DNA double-strand breaks. Carcinogenesis 23 (2002) 687-696
2. Rothkamm K., Kruger I., Thompson L.H., and Lobrich M. Pathways of DNA double-strand break repair during the mammalian cell cycle. Mol. Cell. Biol. 23 (2003) 5706-5715
3. Johnson R.D., and Jasin M. Sister chromatid gene conversion is a prominent double-strand break repair pathway in mammalian cells. EMBO J. 19 (2000) 3398-3407
4. Shrivastav M., Haro L.P.D., and Nickoloff J.A. Regulation of DNA double-strand break repair pathway choice. Cell Res. 18 (2008) 134-147
5. Takata M., Sasaki M.S., Sonoda E., Morrison C., Hashimoto M., Utsumi H., Iwai Y.Y., Shinohara A., and Takeda S. Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. EMBO J. 17 (1998) 5497-5508
6. Adachi N., So S., Iiizumi S., Nomura Y., Murai K., Yamakawa C., Miyagawa K., and Koyama H. The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination. DNA Cell Biol. 25 (2006) 19-24
7. Filippini G., Griffin S., Uhr M., Eppenberger H., Bonilla J., Cavalli F., and Soldati G. A novel flow cytometric method for the quantification of p53 gene expression. Cytometry 31 (1998) 180-186
8. Grawunder U., Zimmer D., Fugmann S., Schwarz K., and Lieber M.R. DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes. Mol. Cell 2 (1998) 477-484
9. Kurosawa A., Koyama H., Takayama S., Miki K., Ayusawa D., Fujii M., Iiizumi S., and Adachi N. The requirement of Artemis in double-strand break repair depends on the type of DNA damage. DNA Cell Biol. 26 (2007) 1-7
10. Schildkraut C.L., and Galgano P.J. Cell synchronization. In: Spector D.L., Goldman R.D., and Leinwand L.A. (Eds). Cells: A Laboratory Manual (1998), Cold Spring Harbor Laboratory Press, New York 14.1-14.13
11. Brown E.H., Iqbal M.A., Stuart S., Hatton K.S., Valinsky J., and Schildkraut C.L. Rate of replication of the murine immunoglobulin heavy-chain locus: evidence that the region is part of a single replicon. Mol. Cell. Biol. 7 (1987) 450-457
12. Iwabuchi K., Hashimoto M., Matsui T., Kurihara T., Shimizu H., Adachi N., Ishiai M., Yamamoto K., Tauchi H., Takata M., Koyama H., and Date T. 53BP1 contributes to survival of cells irradiated with X-ray during G1 without Ku70 or Artemis. Genes Cells 11 (2006) 935-948
13. Adachi N., Kurosawa A., and Koyama H. Highly proficient gene targeting by homologous recombination in the human pre-B cell line Nalm-6. Methods Mol. Biol. 435 (2008) 17-29
14. Buerstedde J.M., and Takeda S. Increased ratio of targeted to random integration after transfection of chicken B cell lines. Cell 67 (1991) 179-188
15. Nojima K., Hochegger H., Saberi A., Fukushima T., Kikuchi K., Yoshimura M., Orelli B.J., Bishop D.K., Hirano S., Ohzeki M., Ishiai M., Yamamoto K., Takata M., Arakawa H., Buerstedde J.M., Yamazoe M., Kawamoto T., Araki K., Takahashi J.A., Hashimoto N., Takeda S., and Sonoda E. Multiple repair pathways mediate tolerance to chemotherapeutic cross-linking agents in vertebrate cells. Cancer Res. 65 (2005) 11704-11711
16. Stucki M., and Jackson S.P. gammaH2AX and MDC1: anchoring the DNA-damage-response machinery to broken chromosomes. DNA Repair (Amst) 5 (2006) 534-543
17. Coquelle A., Mouhamad S., Pequignot M.O., Braun T., Carvalho G., Vivet S., Metivier D., Castedo M., and Kroemer G. Enrichment of non-synchronized cells in the G1, S and G2 phases of the cell cycle for the study of apoptosis. Biochem. Pharmacol. 72 (2006) 1396-1404
18. Ohnuma K., Yomo T., Asashima M., and Kaneko K. Sorting of cells of the same size, shape, and cell cycle stage for a single cell level assay without staining. BMC Cell Biol. 7 (2006) 25